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Cat. No.: HY-139821

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FTO-IN-5

FTO-IN-5 is a potent and selective FTO inhibitor, with an IC₅₀ of 0.087 μM for FTO demethylase activity and a K_d of 28.0 nM for direct binding to FTO. The IC₅₀ of FTO-IN-5 for ALKBH5 is 8.98 μM. FTO-IN-5 can be used in studies related to FTO demethylase activity and cancer.

For research use only. We do not sell to patients.

CAS No.: 2763577-75-7
Formula: C₂₃H₁₈Cl₂N₆O₆
Molecular Weight:545.33

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

FTO-IN-5 Chemical Structure

CAS No.: 2763577-75-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

FTO

0.087 μM (IC₅₀)

FTO

28 μM (Kd)

ALKBH5

8.98 μM (IC₅₀)

In Vitro

FTO-IN-5 (compound 11b) (50 μM) inhibits FTO demethylase activity by 94% in an in vitro HPLC enzymatic activity assay^[1].
FTO-IN-5 inhibits FTO demethylase activity in a dose-dependent manner, with an IC₅₀ of 0.087 μM^[1].
FTO-IN-5 directly binds to FTO in ITC assays, with a K_d of 28.0 nM^[1].
FTO-IN-5 inhibits the demethylase activity of ALKBH5 with an IC₅₀ of 8.98 μM; its selectivity index for FTO relative to ALKBH5 is 103^[1].
FTO-IN-5 (10 μM) does not inhibit KDM4C, whereas the control compound 2,4-PDCA (HY-W017132) (10 μM) exhibits an inhibition rate of 49% against KDM4C, which further supports the FTO selectivity of FTO-IN-5^[1].
FTO-IN-5 exerts a minor effect on cell growth in NOMO-1 acute monocytic leukemia cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2763577-75-7
Molecular Weight 545.33
Formula C₂₃H₁₈Cl₂N₆O₆
SMILES

OC(/C=C/C(NNC(C1=CN=CC=C1NC(C=C2Cl)=C(C)C(Cl)=C2NC3=CC=CC=C3[N+]([O-])=O)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Prakash M, et al. Identification of Potent and Selective Inhibitors of Fat Mass Obesity-Associated Protein Using a Fragment-Merging Approach. Journal of medicinal chemistry. 2021 Nov 11;64(21):15810-15824. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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