Thalidomide-O-amido-PEG3-C2-NH2 hydrochloride

Name: Thalidomide-O-amido-PEG3-C2-NH2 hydrochloride
Brand: MedChemExpress
SKU: HY-107440A
Rating: 4.5 (1 reviews)

Cat. No.: HY-107440A Purity: 98.29%: FAQs
Data Sheet SDS COA Handling Instructions

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Thalidomide-O-amido-PEG3-C2-NH2 hydrochloride is a synthesized E3 ligase ligand-linker conjugate that incorporates the Thalidomide based cereblon ligand and 3-unit PEG linker used in PROTAC technology.

For research use only. We do not sell to patients.

Thalidomide-O-amido-PEG3-C2-NH2 hydrochloride Chemical Structure

CAS No. : 2245697-84-9

Size	Price	Stock	Quantity
25 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Thalidomide-O-amido-PEG3-C2-NH2 hydrochloride:

Thalidomide-O-amido-PEG3-C2-NH2 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Cereblon

In Vitro

Thalidomide-O-amido-PEG3-C2-NH2 hydrochloride is composed of Degron (E3 ubiquitin ligase) and a linker, and they are used in PROTAC technology. Thalidomide-O-amido-PEG3-C2-NH2 binds to the targeting ligand to induce the target protein (including BRD4, BRD2, and BRD3) degradation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

542.97

Formula

C₂₃H₃₁ClN₄O₉

CAS No.

2245697-84-9

Appearance

Solid

Color

Off-white to yellow

SMILES

[H]Cl.NCCOCCOCCOCCNC(COC1=C(C2=CC=C1)C(N(C(CCC3=O)C(N3)=O)C2=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (230.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (184.17 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8417 mL	9.2086 mL	18.4172 mL
5 mM	0.3683 mL	1.8417 mL	3.6834 mL
10 mM	0.1842 mL	0.9209 mL	1.8417 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.29%

Select Batch:

Data Sheet (269 KB) SDS (393 KB)

COA (245 KB) HNMR (309 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Methods to induce targeted protein degradation through bifunctional molecules. WO2017007612A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.8417 mL	9.2086 mL	18.4172 mL	46.0431 mL
	5 mM	0.3683 mL	1.8417 mL	3.6834 mL	9.2086 mL
	10 mM	0.1842 mL	0.9209 mL	1.8417 mL	4.6043 mL
	15 mM	0.1228 mL	0.6139 mL	1.2278 mL	3.0695 mL
	20 mM	0.0921 mL	0.4604 mL	0.9209 mL	2.3022 mL
	25 mM	0.0737 mL	0.3683 mL	0.7367 mL	1.8417 mL
	30 mM	0.0614 mL	0.3070 mL	0.6139 mL	1.5348 mL
	40 mM	0.0460 mL	0.2302 mL	0.4604 mL	1.1511 mL
	50 mM	0.0368 mL	0.1842 mL	0.3683 mL	0.9209 mL
	60 mM	0.0307 mL	0.1535 mL	0.3070 mL	0.7674 mL
	80 mM	0.0230 mL	0.1151 mL	0.2302 mL	0.5755 mL
	100 mM	0.0184 mL	0.0921 mL	0.1842 mL	0.4604 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.