Thioacetamide (Synonyms: Acetothioamide; TAA; Thiacetamide)

Cat. No.: HY-Y0698 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Thioacetamide (TAA) is an indirect hepatotoxin and causes parenchymal cell necrosis. Thioacetamide requires metabolic activation by microsomal CYP2E1 to thioacetamide-S-oxide initially and then to thioacetamide-S-dioxide, which is a highly reactive metabolite, and its reactive metabolites covalently bind to proteins and lipids thereby causing oxidative stress and centrilobular necrosis. Thioacetamide can induce chronic liver fibrosis, encephalopathy and other events model.

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Thioacetamide Chemical Structure

CAS No. : 62-55-5

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Thioacetamide (TAA; 0-10000 μM; 24 h; WB-F344 cells) has cytotoxicity in a concentration-dependent manner^[4].
Thioacetamide (TAA; 1000 and 10000 μM; 0-24 h; WB-F344 cells) has differentially-expressed genes in the early phases at low (1000 μM) and high (10000 μM) concentrations^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	WB-F344 cells
Concentration:	0-10000 μM
Incubation Time:	24 hours
Result:	Had 20% and 50% cell death at the 1000 and 10000 μM concentrations, respectively.

In Vivo

Thioacetamide (TAA; 100 mg/kg; i.p.; three times weekly) can induce chronic liver fibrosis in male ICR mice^[2].
Thioacetamide (200-1200 mg/kg; i.p.; once) induces hepatic encephalopathy model in C57BL/6 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ICR mice^[2]
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection; three times weekly for eight weeks
Result:	Induced chronic liver fibrosis in male ICR mice and resulted in lower body weight, serum cholesterol and triglycerides as well as increased liver size, ALT, AST and LDH values.

Animal Model:	Male C57BL/6 mice (20-25g, aged 8-12 weeks)^[3]
Dosage:	200, 600, and 1,200 mg/kg
Administration:	Intraperitoneal injection; once
Result:	Altered the neuropsychiatric state, motor behavior and reflex and sensory functions. Increased in the glutamate release in the cerebral cortex of Hepatic encephalopathy (HE) mice.

Molecular Weight

75.13

Formula

C₂H₅NS

CAS No.

62-55-5

Appearance

Solid

Color

White to off-white

SMILES

CC(N)=S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (1331.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (665.51 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	13.3103 mL	66.5513 mL	133.1026 mL
5 mM	2.6621 mL	13.3103 mL	26.6205 mL
10 mM	1.3310 mL	6.6551 mL	13.3103 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (33.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (33.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (33.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (297 KB) SDS (618 KB)

COA (246 KB) HNMR (112 KB)

Handling Instructions (2659 KB)

References

[1]. Wallace MC, et, al. Standard operating procedures in experimental liver research: thioacetamide model in mice and rats. Lab Anim. 2015 Apr;49(1 Suppl):21-9. [Content Brief]

[2]. Chen IS, et, al. Hepatoprotection of silymarin against thioacetamide-induced chronic liver fibrosis. J Sci Food Agric. 2012 May;92(7):1441-7. [Content Brief]

[3]. Miranda AS, et, al. A thioacetamide-induced hepatic encephalopathy model in C57BL/6 mice: a behavioral and neurochemical study. Arq Neuropsiquiatr. 2010 Aug;68(4):597-602. [Content Brief]

[4]. Yeom HJ, et, al. Expression analysis of early response-related genes in rat liver epithelial cells exposed to thioacetamide in vitro. J Vet Med Sci. 2009 Jun;71(6):719-27. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	13.3103 mL	66.5513 mL	133.1026 mL	332.7566 mL
	5 mM	2.6621 mL	13.3103 mL	26.6205 mL	66.5513 mL
	10 mM	1.3310 mL	6.6551 mL	13.3103 mL	33.2757 mL
	15 mM	0.8874 mL	4.4368 mL	8.8735 mL	22.1838 mL
	20 mM	0.6655 mL	3.3276 mL	6.6551 mL	16.6378 mL
	25 mM	0.5324 mL	2.6621 mL	5.3241 mL	13.3103 mL
	30 mM	0.4437 mL	2.2184 mL	4.4368 mL	11.0919 mL
	40 mM	0.3328 mL	1.6638 mL	3.3276 mL	8.3189 mL
	50 mM	0.2662 mL	1.3310 mL	2.6621 mL	6.6551 mL
	60 mM	0.2218 mL	1.1092 mL	2.2184 mL	5.5459 mL
	80 mM	0.1664 mL	0.8319 mL	1.6638 mL	4.1595 mL
	100 mM	0.1331 mL	0.6655 mL	1.3310 mL	3.3276 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Thioacetamide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thioacetamide62-55-5Acetothioamide TAA ThiacetamideNecroptosisindirect hepatotoxincell necrosisCYP2E1thioacetamide-S-oxide initiallythioacetamide-S-dioxideoxidative stresscentrilobular necrosischronic liver fibrosisInhibitorinhibitorinhibit

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