TMA chloride, for molecular biology, 99%(T)
Based on 1 Customer Validation
TMA chloride, for molecular biology, 99%(T) (Tetramethylammonium chloride, for molecular biology, 99%(T)) can increase the specificity of hybridization and raise the DNA denaturation temperature in PCR.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 75-57-0
- Formula: C4H12ClN
- Molecular Weight:109.60
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| MDCK | IC50 |
1138 μM
Compound: Tetramethylammonium
|
TP_TRANSPORTER: inhibition of TEA uptake (TEA: 50 uM) in OCT1-expressing MDCK cells
TP_TRANSPORTER: inhibition of TEA uptake (TEA: 50 uM) in OCT1-expressing MDCK cells
|
[PMID: 11758759] |
| MDCK | IC50 |
1674 μM
Compound: Tetramethylammonium
|
TP_TRANSPORTER: inhibition of TEA uptake (TEA: 50 uM) in OCT2-expressing MDCK cells
TP_TRANSPORTER: inhibition of TEA uptake (TEA: 50 uM) in OCT2-expressing MDCK cells
|
[PMID: 11758759] |
| Oocyte | IC50 |
150 μM
Compound: Tetramethylammonium
|
TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in Xenopus laevis oocytes
TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in Xenopus laevis oocytes
|
[PMID: 12240953] |
| Oocyte | IC50 |
630 μM
Compound: Tetramethylammonium
|
TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes
TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes
|
[PMID: 11502595] |
Chemical Information
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CAS No. 75-57-0
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Appearance Solid
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Molecular Weight 109.60
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Formula C4H12ClN
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Color White to off-white
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SMILES
C[N+](C)(C)C.[Cl-]
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Synonyms
Tetramethylammonium chloride, for molecular biology, 99%(T)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (912.41 mM)
DMSO : 5.83 mg/mL (53.19 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (240 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 9.1241 mL | 45.6204 mL | 91.2409 mL | 228.1022 mL |
| 5 mM | 1.8248 mL | 9.1241 mL | 18.2482 mL | 45.6204 mL | |
| 10 mM | 0.9124 mL | 4.5620 mL | 9.1241 mL | 22.8102 mL | |
| 15 mM | 0.6083 mL | 3.0414 mL | 6.0827 mL | 15.2068 mL | |
| 20 mM | 0.4562 mL | 2.2810 mL | 4.5620 mL | 11.4051 mL | |
| 25 mM | 0.3650 mL | 1.8248 mL | 3.6496 mL | 9.1241 mL | |
| 30 mM | 0.3041 mL | 1.5207 mL | 3.0414 mL | 7.6034 mL | |
| 40 mM | 0.2281 mL | 1.1405 mL | 2.2810 mL | 5.7026 mL | |
| 50 mM | 0.1825 mL | 0.9124 mL | 1.8248 mL | 4.5620 mL | |
| H2O | 60 mM | 0.1521 mL | 0.7603 mL | 1.5207 mL | 3.8017 mL |
| 80 mM | 0.1141 mL | 0.5703 mL | 1.1405 mL | 2.8513 mL | |
| 100 mM | 0.0912 mL | 0.4562 mL | 0.9124 mL | 2.2810 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.