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Cat. No.: HY-187098

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TopoII/Tubulin-IN-2

TopoII/Tubulin-IN-2 is a Tubulin polymerization inhibitor and a Topoisomerase II activity inhibitor. TopoII/Tubulin-IN-2 induces G2 and M phase arrest of the cell cycle. TopoII/Tubulin-IN-2 promotes cell Apoptosis. TopoII/Tubulin-IN-2 can be used for the research of breast adenocarcinoma and liver cancer.

For research use only. We do not sell to patients.

Formula: C₂₄H₂₃ClN₂O₆
Molecular Weight:470.90

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

TopoII/Tubulin-IN-2 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

Topoisomerase II

All Topoisomerase Isoforms :

In Vitro

TopoII/Tubulin-IN-2 (Compound 20) potently inhibits the proliferation of MCF7 cells with an IC₅₀ of 2.4 μM, moderately inhibits HepG2 cells with an IC₅₀ of 6.1 μM, and inhibits HCT116 cells with an IC₅₀ of 34.4 μM. It also exhibits selectivity toward normal BJ1 cells^[1].
TopoII/Tubulin-IN-2 inhibits tubulin polymerization in MCF7 cells by 74.7%^[1].
TopoII/Tubulin-IN-2 moderately inhibits the activity of topoisomerase II in MCF7 cells, with an inhibition rate of 70.3%^[1].
TopoII/Tubulin-IN-2 (2.4 μM) induces G₂/M phase arrest in MCF7 cells, with 43.3% of cells arrested at the G₂/M phase^[1].
TopoII/Tubulin-IN-2 (2.4 μM; 48 h) induces significant apoptosis in MCF7 cells, with total apoptotic cells accounting for 38.41% and extremely low necrosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

MCF7 human breast adenocarcinoma
Concentration:

2.4 μM
Incubation Time:

48 h
Result:

Induced total apoptosis in 38.41% of cells, including 11.91% early apoptosis and 22.63% late apoptosis, with only 3.87% necrosis.
Untreated control cells showed only 3.04% total apoptosis.

Chemical Information

Molecular Weight 470.90
Formula C₂₄H₂₃ClN₂O₆
SMILES

N#CC1=C(Cl)N=C(C2=CC(OC)=C(OC)C(OC)=C2)C=C1C3=CC(OC)=C(OC)C(OC)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hassan ES, et al. Nicotinonitrile based dual inhibitors of tubulin and topoisomerase II exhibit potent anticancer activity. Scientific reports. 2026 Jun 25;16(1):19595. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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