1. Cell Cycle/DNA Damage Apoptosis
  2. Topoisomerase Apoptosis
  3. Topoisomerase IIα-IN-4

Topoisomerase IIα-IN-4 (F2) is a non-intercalative ATP-competitive human DNA topoisomerase II inhibitor with an IC50 value of 3.8 and 10.1 μM for TopoIIα and TopoIIβ, respectively. Topoisomerase IIα-IN-4 shows potent potency in apoptosis induction and cell cycle arrest in HepG2 cells. Topoisomerase IIα-IN-4 exhibits strong antitumor activities against human cancer cell lines, it can be used for the research of cancer.

For research use only. We do not sell to patients.

Topoisomerase IIα-IN-4 Chemical Structure

Topoisomerase IIα-IN-4 Chemical Structure

CAS No. : 2860554-26-1

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Top Publications Citing Use of Products

View All Topoisomerase Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Topoisomerase IIα-IN-4 (F2) is a non-intercalative ATP-competitive human DNA topoisomerase II inhibitor with an IC50 value of 3.8 and 10.1 μM for TopoIIα and TopoIIβ, respectively. Topoisomerase IIα-IN-4 shows potent potency in apoptosis induction and cell cycle arrest in HepG2 cells. Topoisomerase IIα-IN-4 exhibits strong antitumor activities against human cancer cell lines, it can be used for the research of cancer[1].

IC50 & Target

topoisomerase II alpha

3.8 μM (IC50)

topoisomerase II beta

10.1 μM (IC50)

In Vitro

Topoisomerase IIα-IN-4 (0-50 μM; 72 h) shows antiproliferative activities against cancer cells[1].
Topoisomerase IIα-IN-4 shows high inhibitory activity and subtypeselectivity against TopoIIα and β with IC50s of 3.8 and 10.1 μM, respectively[1].
Topoisomerase IIα-IN-4 (0.3 μM; 4 h) is a non-intercalative TopoIIα catalytic inhibitor[1].
Topoisomerase IIα-IN-4 (0.5-1 μM; 48 h) induces cell apoptosis[1].
Topoisomerase IIα-IN-4 (0.5-1 μM; 24 h) induces cell-cycle arrest[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: HeLa, HCT-116, MDA-MB231, HepG2, A549, CCL-226, BEAS-2B and HL-7702 cell lines
Concentration: 0-50 μM
Incubation Time: 72 hours
Result: Inhibited HeLa, HCT-116, MDA-MB 231, HepG2, A549, CCL-226, BEAS-2B and HL-7702 cells with IC50s of 0.1, 0.2, 0.3, 0.2, 0.3, 0.3, >50, 31.9 and 16.7 μM, respectively.

Western Blot Analysis[1]

Cell Line: HepG-2 cell line
Concentration: 0.3 μM
Incubation Time: 4 hours
Result: Showed no effect on the level of phospho-histone H2AX.

Apoptosis Analysis[1]

Cell Line: 0.5-1 μM
Concentration: 0.3 μM
Incubation Time: 48 hours
Result: Increased the total numbers of early and late apoptotic cells from 6.0% to 70.6% at the concentration of 1 μM.
In Vivo

Topoisomerase IIα-IN-4 (500 mg/kg; p.o. twice at the first day) shows no acute toxicity in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice[1]
Dosage: 500 mg/kg
Administration: Oral gavage; 500 mg/kg twice at the first day
Result: Exerted lower toxicity in this established test and caused no significant difference of body weight of mice.
Molecular Weight

367.44

Formula

C25H21NO2

CAS No.
SMILES

O=C(NC1=CC=C(C2=CC=C3C=C(O)C=CC3=C2)C=C1)CCC4=CC=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Topoisomerase IIα-IN-4 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Topoisomerase IIα-IN-4
Cat. No.:
HY-151453
Quantity:
MCE Japan Authorized Agent: