Tris(1-chloro-2-propyl) phosphate
Based on 1 Customer Validation
Tris (1-chloro-2-propyl) phosphate (Tris (1-chloropropan-2-yl) phosphate) is a chlorinated organophosphate flame retardant. Tris (1-chloro-2-propyl) phosphate induces DNA damage, elevates intracellular ROS levels, and triggers oxidative stress. Tris (1-chloro-2-propyl) phosphate disrupts mitochondrial membrane potential, leading to mitochondrial dysfunction. Tris (1-chloro-2-propyl) phosphate can trigger cell Apoptosis. Tris (1-chloro-2-propyl) phosphate reduces the survival rate of umbilical vein endothelial cells at relatively high concentrations.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 13674-84-5
- Formula: C9H18Cl3O4P
- Molecular Weight:327.57
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Tris (1-chloro-2-propyl) phosphate (200-400 μM; 24 h) reduces the viability of human umbilical vein endothelial cells (HUVEC), as determined by the MTT assay[1].
Tris (1-chloro-2-propyl) phosphate (200-400 μM; 24 h) reduces the viability of human umbilical vein endothelial cells (HUVEC), as confirmed by the NRU assay[1].
Tris (1-chloro-2-propyl) phosphate (50-400 μM; 24 h) induces significant DNA damage in human umbilical vein endothelial cells (HUVECs), with olive tail moment (OTM) values of 1.03, 2.89, 20.24, and 35.59, respectively[1].
Tris (1-chloro-2-propyl) phosphate (25-400 μM; 24 h) significantly increases the level of reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs)[1].
Tris (1-chloro-2-propyl) phosphate (5-400 μM; 24 h) induces apoptosis in human umbilical vein endothelial cells (HUVECs)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human umbilical vein endothelial cells (HUVECs)
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Concentration:5, 10, 25, 50, 100, 200, 400 μM
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Incubation Time:24 h
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Result:Caused a 15.27% significant decline in cell survival relative to control HUVECs (MTT).
Caused a 20.56% significant decline in cell survival relative to control HUVECs (MTT).\nCaused a 21.67% significant decline in cell survival relative to control HUVECs (NRU).
Caused a 48.67% significant decline in cell survival relative to control HUVECs (NRU).
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Cell Line:human umbilical vein endothelial cells (HUVECs)
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Concentration:5, 10, 25, 50, 100, 200, 400 μM
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Incubation Time:24 h
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Result:Resulted in 5.1% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Resulted in 5.9% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Resulted in 6.3% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Resulted in 5.5% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Resulted in 6.8% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Resulted in 15.8% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Resulted in 58.8% of cells in the SubG1 apoptotic phase, significantly higher than control 3.1%.
Chemical Information
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CAS No. 13674-84-5
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Appearance Liquid
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Molecular Weight 327.57
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Formula C9H18Cl3O4P
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Color Colorless to light yellow
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SMILES
O=P(OC(C)CCl)(OC(C)CCl)OC(C)CCl
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Synonyms
Tris(1-chloropropan-2-yl) phosphate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 200 mg/mL (610.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (15.26 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (15.26 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0528 mL | 15.2639 mL | 30.5278 mL | 76.3196 mL |
| 5 mM | 0.6106 mL | 3.0528 mL | 6.1056 mL | 15.2639 mL | |
| 10 mM | 0.3053 mL | 1.5264 mL | 3.0528 mL | 7.6320 mL | |
| 15 mM | 0.2035 mL | 1.0176 mL | 2.0352 mL | 5.0880 mL | |
| 20 mM | 0.1526 mL | 0.7632 mL | 1.5264 mL | 3.8160 mL | |
| 25 mM | 0.1221 mL | 0.6106 mL | 1.2211 mL | 3.0528 mL | |
| 30 mM | 0.1018 mL | 0.5088 mL | 1.0176 mL | 2.5440 mL | |
| 40 mM | 0.0763 mL | 0.3816 mL | 0.7632 mL | 1.9080 mL | |
| 50 mM | 0.0611 mL | 0.3053 mL | 0.6106 mL | 1.5264 mL | |
| 60 mM | 0.0509 mL | 0.2544 mL | 0.5088 mL | 1.2720 mL | |
| 80 mM | 0.0382 mL | 0.1908 mL | 0.3816 mL | 0.9540 mL | |
| 100 mM | 0.0305 mL | 0.1526 mL | 0.3053 mL | 0.7632 mL |
Tris(1-chloro-2-propyl) phosphate Related Classifications
HY-W023144 Related Classifications
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- Tris(1-chloro-2-propyl)
- 13674-84-5
- Tris(1-chloropropan-2-yl)
- Environmental Pollutants
- Reactive Oxygen Species (ROS)
- DNA/RNA Synthesis
- Biochemical Assay Reagents
- Apoptosis
- Mitochondrial Metabolism
- reactive oxygen species
- human umbilical vein endothelial cells
- mitochondrial membrane potential
- apoptotic cell death
- mitochondrial dysfunction
- human vascular system
- oxidative stress
- DNA damage
- SubG1 phase
- Inhibitor
- inhibitor
- inhibit