Cat. No.: HY-W014901

Bisphenol F

(Synonyms: BPF; 4,4'-Dihydroxydiphenylmethane)

Customer Review

Based on 1 Customer Validation

Bisphenol F is an orally active endocrine disruptor. Bisphenol F promotes ROS generation, upregulates p-AKT/p-GSK3β, and induces Apoptosis. Bisphenol F interferes with glucose metabolism, affects neurodevelopment and reproductive function. Bisphenol F reduces social novelty preference in mouse offspring. Bisphenol F can be used in bone, blood, and fat-related studies. Bisphenol F is used as a substitute for Bisphenol A (HY-18260).

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 620-92-8
Formula: C₁₃H₁₂O₂
Molecular Weight:200.24

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Bisphenol F Chemical Structure

CAS No.: 620-92-8

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[7]

GSK-3β

All Akt Isoforms :

All GSK-3 Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	909 nM Compound: 2	Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	>10000 nM Compound: 2	Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay	[PMID: 30940565]
HEK293	IC₅₀	>10000 nM Compound: 2	Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	237 μM Compound: 1	Agonist activity at MT1 receptor (unknown origin) expressed in HEK293 cells by Fluo-8 dye-based calcium assay Agonist activity at MT1 receptor (unknown origin) expressed in HEK293 cells by Fluo-8 dye-based calcium assay	[PMID: 31204226]
HEK293	EC₅₀	244 μM Compound: 1	Agonist activity at MT2 receptor (unknown origin) expressed in HEK293 cells by Fluo-8 dye-based calcium assay Agonist activity at MT2 receptor (unknown origin) expressed in HEK293 cells by Fluo-8 dye-based calcium assay	[PMID: 31204226]
HeLa S3	IC₅₀	>10 μM Compound: 15	Cytotoxicity against human HeLa S3 cells assessed as cell viability incubated for 48 hrs by microscopic analysis Cytotoxicity against human HeLa S3 cells assessed as cell viability incubated for 48 hrs by microscopic analysis	[PMID: 35704432]

In Vitro

Bisphenol F (0-100 µM; 24 h) significantly reduces cell viability, induces oxidative stress (increased ROS, decreased antioxidant enzyme activity) in human granulosa KGN cells^[1].
Bisphenol F (0.1-10 µM; 24 h) significantly inhibits proliferation via apoptosis induction in human osteoblasts, reducing alkaline phosphatase activity and mineralization capacity^[2].
Bisphenol F (0.1-500 µg/mL; 1-24 h) induces ROS in human red blood cells^[3].
Bisphenol F (10 nM-1 µM; 12 d) inhibits late differentiation gene expression and reduces lipid accumulation in 3T3-L1 preadipocytes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[4]

Cell Line:

Murine 3T3-L1 preadipocytes
Concentration:

10 nM, 1 µM
Incubation Time:

12 d
Result:

Downregulated the mRNA levels of Cfd and Plin at day 12 of differentiation.

In Vivo

Bisphenol F (administered to pregnant female mice; 40-4000 μg/kg; p.o. via drinking water; from gestation to F1 generation 3 weeks old) induces testicular injury, decreases testosterone levels, and impairs sperm quality in F1 generation male Kunming mice^[6].
Bisphenol F (100 µg/kg; i.g.; every other day; 5 months) improves glucose tolerance, insulin sensitivity, inhibits hepatic gluconeogenesis, and increases TCA cycle metabolites in high-fat diet-fed mice^[7].
Bisphenol F (50 µg/kg; s.c.; daily) decreases social novelty preference in C57BL/6J mouse offspring without affecting anxiety/depression-like behaviors^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6J mice (8-10 weeks old, ~25 g); high-fat diet model^[7]
Dosage:

100 µg/kg
Administration:

Gavage (i.g.); every other day; 5 months
Result:

Reduced fasting blood glucose by ~20% in HFD mice.
Enhanced insulin signaling in skeletal muscle (p-AKT/p-GSK3β upregulation), and decreased hepatic G6pase/Pepck mRNA expression by ~40-50%.

Chemical Information

CAS No. 620-92-8
Appearance Solid
Molecular Weight 200.24
Formula C₁₃H₁₂O₂
Color White to off-white
SMILES

OC1=CC=C(CC2=CC=C(O)C=C2)C=C1
Synonyms

BPF; 4,4'-Dihydroxydiphenylmethane
Structure Classification
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (499.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.9940 mL

24.9700 mL

49.9401 mL

5 mM

0.9988 mL

4.9940 mL

9.9880 mL

10 mM

0.4994 mL

2.4970 mL

4.9940 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (762 KB)
- English - EN (762 KB)
- Français - FR (762 KB)
- Deutsch - DE (762 KB)
- Norwegian - NO (762 KB)
- Español - ES (762 KB)
- Swedish - SV (762 KB)
- Italian - IT (762 KB)
- Korean - KR (762 KB)
- Portuguese - PT (762 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Huang M, et al. Bisphenol A and its analogues bisphenol S, bisphenol F and bisphenol AF induce oxidative stress and biomacromolecular damage in human granulosa KGN cells. Chemosphere. 2020 Aug;253:126707. [Content Brief]

[2]. García-Recio E, et al. Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts. Arch Toxicol. 2023 Jul;97(7):1899-1905. [Content Brief]

[3]. Maćczak A, et al. Bisphenol A, bisphenol S, bisphenol F and bisphenol AF induce different oxidative stress and damage in human red blood cells (in vitro study). Toxicol In Vitro. 2017 Jun;41:143-149. [Content Brief]

[4]. Drobna Z, et al. Bisphenol F has different effects on preadipocytes differentiation and weight gain in adult mice as compared with Bisphenol A and S. Toxicology. 2019 May 15;420:66-72. [Content Brief]

[5]. Kim Y, et al. Mechanism of Bisphenol F Affecting Motor System and Motor Activity in Zebrafish. Toxics. 2023 May 24;11(6):477. [Content Brief]

[6]. Li B, et al. Effect of bisphenol F on reproductive function in F1 generation male mice and its potential mechanisms. Environ Pollut. 2025 Feb 15;367:125546. [Content Brief]

[7]. Lv Z, et al. In vivo hypoglycemic effects of bisphenol F exposure in high-fat diet mice. Chemosphere. 2023 Jan;311(Pt 1):137066. [Content Brief]

[8]. Moon HJ, et al. Effects of prenatal bisphenol S and bisphenol F exposure on behavior of offspring mice. Anim Cells Syst (Seoul). 2023 Oct 11;27(1):260-271. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.9940 mL	24.9700 mL	49.9401 mL	124.8502 mL
	5 mM	0.9988 mL	4.9940 mL	9.9880 mL	24.9700 mL
	10 mM	0.4994 mL	2.4970 mL	4.9940 mL	12.4850 mL
	15 mM	0.3329 mL	1.6647 mL	3.3293 mL	8.3233 mL
	20 mM	0.2497 mL	1.2485 mL	2.4970 mL	6.2425 mL
	25 mM	0.1998 mL	0.9988 mL	1.9976 mL	4.9940 mL
	30 mM	0.1665 mL	0.8323 mL	1.6647 mL	4.1617 mL
	40 mM	0.1249 mL	0.6243 mL	1.2485 mL	3.1213 mL
	50 mM	0.0999 mL	0.4994 mL	0.9988 mL	2.4970 mL
	60 mM	0.0832 mL	0.4162 mL	0.8323 mL	2.0808 mL
	80 mM	0.0624 mL	0.3121 mL	0.6243 mL	1.5606 mL
	100 mM	0.0499 mL	0.2497 mL	0.4994 mL	1.2485 mL

Keywords

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Bisphenol F

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

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Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

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Anti-inflammatory Traditional Chinese Medicine Active Compound Library

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Cell Death Library

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MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

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High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Lactylation Compound Library

Drug Fragment Library

All Akt Isoforms

All GSK-3 Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bisphenol F Related Classifications

HY-W014901 Related Classifications