α1-3,4,6 Galactosidase 

α1-3,4,6 Galactosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1-3, α1-4 and α1-6-linked galactose residues from oligosaccharides^[1].

others

Product Information
This product is a recombinant expression of E.coli, an N-terminal His Tag with a molecular weight of 40 kDa. The storage buffer consists of 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, and 10% glycerol. α1-3,4,6-galactosidase is a broadly specific exoglycosidase that catalyzes the hydrolysis of terminally non-reducing α1-3, α1-4, and α1-6-linked galactose residues in oligosaccharides.

Instructions for Use
1. Add the substrate, 50 mM sodium acetate, and 5 mM CaCl2 to the reaction system sequentially. Mix well, then add an appropriate amount of α1-3,4,6-galactosidase. 2. Incubate the sample at 37°C for 2-4 hours or overnight.

Precautions
When scaling up the system, the incubation time or enzyme amount needs to be adjusted according to the actual situation. Low-speed shaking can be used to mix the components and increase the conversion rate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9025-35-8

Liquid

Colorless to light yellow

[a1-3,4,6 Galactosidase]

≥20000U/mL

One unit is defined as the amount of enzyme that releases 1 nmol of reducing sugar per hour from Galα1-3Galβ1-4Gal at 37°C at pH 5.5.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lyudmila Velkova, et al. Glycan structures of the structural subunit (HtH1) of Haliotis tuberculata hemocyanin. Glycoconj J. 2011 Aug;28(6):385-95.  [Content Brief]