α1-3,4,6 Galactosidase

Product Information
This product is a recombinant expression of E.coli, an N-terminal His Tag with a molecular weight of 40 kDa. The storage buffer consists of 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, and 10% glycerol. α1-3,4,6-galactosidase is a broadly specific exoglycosidase that catalyzes the hydrolysis of terminally non-reducing α1-3, α1-4, and α1-6-linked galactose residues in oligosaccharides.

Instructions for Use
1. Add the substrate, 50 mM sodium acetate, and 5 mM CaCl2 to the reaction system sequentially. Mix well, then add an appropriate amount of α1-3,4,6-galactosidase. 2. Incubate the sample at 37°C for 2-4 hours or overnight.

Precautions
When scaling up the system, the incubation time or enzyme amount needs to be adjusted according to the actual situation. Low-speed shaking can be used to mix the components and increase the conversion rate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.