14-3-3η Protein inhibitor 1

Name: 14-3-3η Protein inhibitor 1
Brand: MedChemExpress
SKU: HY-146302
Rating: 5.0 (2 reviews)

Art. -Nr.: HY-146302 Reinheit: 99.00%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

14-3-3η Protein inhibitor 1 (Compound C11) is a 14-3-3η protein inhibitor with a K_D of 35 µM. 14-3-3η Protein inhibitor 1 shows inhibitory activities against several typical human liver cancer cell lines. 14-3-3η Protein inhibitor 1 induces cell apoptosis and G1-S cell cycle arrest with good metabolic stability.

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14-3-3η Protein inhibitor 1 Chemische Struktur

CAS. Nr. : 2770842-10-7

Größe	Verfügbarkeit	Menge

Kostenlose Probe (0.1 - 0.2 mg)	Jetzt bewerben
Kostenlose Probe (0.1 - 0.2 mg)	Jetzt bewerben
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	Auf Lager	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Auf Lager	Estimated Time of Arrival: December 31
Solid
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
25 mg	Auf Lager	Estimated Time of Arrival: December 31
50 mg	Auf Lager	Estimated Time of Arrival: December 31
100 mg	Angebot einholen
200 mg	Angebot einholen

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Kundenbewertung

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 14-3-3η Protein inhibitor 1

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität
Reinheit & Dokumentation
Verweise
Kundenbewertung

Beschreibung

IC₅₀ & Target

K_D: 35 µM (14-3-3η)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	5.92 μM Compound: C11	Antiproliferative activity against human Bel-7402 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human Bel-7402 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
Bel7402/5-FU	IC₅₀	4.55 μM Compound: C11	Antiproliferative activity against human BEL-7402/5-FU cells overexpressing 14-3-3 eta assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human BEL-7402/5-FU cells overexpressing 14-3-3 eta assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
Hep 3B2	IC₅₀	4.07 μM Compound: C11	Antiproliferative activity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
HepG2	IC₅₀	3.43 μM Compound: C11	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
SMMC-7721	IC₅₀	2.92 μM Compound: C11	Antiproliferative activity against human SMMC-7721 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human SMMC-7721 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
SNU-387	IC₅₀	5.47 μM Compound: C11	Antiproliferative activity against human SNU-387 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human SNU-387 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]

Molekulargewicht

456.58

Formel

C₂₉H₃₂N₂O₃

CAS. Nr.

2770842-10-7

Appearance

Solid

Color

White to off-white

SMILES

COC1=CC=CC(CNCC2=C(N(C3=C2C=CC(C(C)C)=C3)CC4=CC=C(C=C4)C)C(O)=O)=C1

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 16.67 mg/mL (36.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1902 mL	10.9510 mL	21.9020 mL
5 mM	0.4380 mL	2.1902 mL	4.3804 mL
10 mM	0.2190 mL	1.0951 mL	2.1902 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 1.67 mg/mL (3.66 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

This protocol yields a clear solution of 1.67 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: 99.00%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (257 KB) HNMR (114 KB) RP-HPLC (170 KB) LCMS (233 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Gao Z, et al. Design, synthesis and antitumor evaluation of novel 1H-indole-2-carboxylic acid derivatives targeting 14-3-3η protein. Eur J Med Chem. 2022 Apr 29;238:114402. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1902 mL	10.9510 mL	21.9020 mL	54.7549 mL
	5 mM	0.4380 mL	2.1902 mL	4.3804 mL	10.9510 mL
	10 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4755 mL
	15 mM	0.1460 mL	0.7301 mL	1.4601 mL	3.6503 mL
	20 mM	0.1095 mL	0.5475 mL	1.0951 mL	2.7377 mL
	25 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	30 mM	0.0730 mL	0.3650 mL	0.7301 mL	1.8252 mL