Cat. No.: HY-146302

14-3-3η Protein inhibitor 1

Name: 14-3-3η Protein inhibitor 1
Brand: MedChemExpress
SKU: HY-146302
Price: 300.00 USD
Availability: InStock
Rating: 5 (2 reviews)

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

14-3-3η Protein inhibitor 1 (Compound C11) is a 14-3-3η protein inhibitor with a K_D of 35 µM. 14-3-3η Protein inhibitor 1 shows inhibitory activities against several typical human liver cancer cell lines. 14-3-3η Protein inhibitor 1 induces cell apoptosis and G1-S cell cycle arrest with good metabolic stability.

For research use only. We do not sell to patients.

Purity : 99.00%
CAS No.: 2770842-10-7
Formula: C₂₉H₃₂N₂O₃
Molecular Weight:456.58

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

14-3-3η Protein inhibitor 1 Chemical Structure

CAS No.: 2770842-10-7

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

14-3-3η

All 14-3-3 Protein Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	5.92 μM Compound: C11	Antiproliferative activity against human Bel-7402 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human Bel-7402 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
Bel7402/5-FU	IC₅₀	4.55 μM Compound: C11	Antiproliferative activity against human BEL-7402/5-FU cells overexpressing 14-3-3 eta assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human BEL-7402/5-FU cells overexpressing 14-3-3 eta assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
Hep 3B2	IC₅₀	4.07 μM Compound: C11	Antiproliferative activity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
HepG2	IC₅₀	3.43 μM Compound: C11	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
SMMC-7721	IC₅₀	2.92 μM Compound: C11	Antiproliferative activity against human SMMC-7721 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human SMMC-7721 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]
SNU-387	IC₅₀	5.47 μM Compound: C11	Antiproliferative activity against human SNU-387 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human SNU-387 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35525080]

Chemical Information

CAS No. 2770842-10-7
Appearance Solid
Molecular Weight 456.58
Formula C₂₉H₃₂N₂O₃
Color White to off-white
SMILES

COC1=CC=CC(CNCC2=C(N(C3=C2C=CC(C(C)C)=C3)CC4=CC=C(C=C4)C)C(O)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Sci Rep

Activation of PPAR-α attenuates myocardial ischemia/reperfusion injury by inhibiting ferroptosis and mitochondrial injury via upregulating 14-3-3η. [Abstract]

2024 Jul 2;14(1):15246. PMID: 38956068
Biomed Pharmacother

Epigallocatechin-3-gallate confers protection against myocardial ischemia/reperfusion injury by inhibiting ferroptosis, apoptosis, and autophagy via modulation of 14-3-3η. [Abstract]

2024 May:174:116542. PMID: 38574620

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (36.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1902 mL

10.9510 mL

21.9020 mL

5 mM

0.4380 mL

2.1902 mL

4.3804 mL

10 mM

0.2190 mL

1.0951 mL

2.1902 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 1.67 mg/mL (3.66 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

This protocol yields a clear solution of 1.67 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Gao Z, et al. Design, synthesis and antitumor evaluation of novel 1H-indole-2-carboxylic acid derivatives targeting 14-3-3η protein. Eur J Med Chem. 2022 Apr 29;238:114402. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1902 mL	10.9510 mL	21.9020 mL	54.7549 mL
	5 mM	0.4380 mL	2.1902 mL	4.3804 mL	10.9510 mL
	10 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4755 mL
	15 mM	0.1460 mL	0.7301 mL	1.4601 mL	3.6503 mL
	20 mM	0.1095 mL	0.5475 mL	1.0951 mL	2.7377 mL
	25 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	30 mM	0.0730 mL	0.3650 mL	0.7301 mL	1.8252 mL

Bioz

Keywords

14-3-3η Protein inhibitor 1

2 Cited Publications

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14-3-3η Protein inhibitor 1

Publications Citing Use of MedChemExpress (MCE) 14-3-3η Protein inhibitor 1

All 14-3-3 Protein Isoforms