7,8-Dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one
Based on 1 publication(s) in Google Scholar
7,8-Dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one (7,8,4'-Trihydroxyisoflavone) is a product that can be isolated from .
For research use only. We do not sell to patients.
- Purity : 99.49%
- CAS No.: 75187-63-2
- Formula: C15H10O5
- Molecular Weight:270.24
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Storage:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 7,8-Dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Melan-a | IC50 |
12.23 μM
Compound: 1
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Inhibition of melanin formation in mouse Melan-a cells after 5 days by ELISA
Inhibition of melanin formation in mouse Melan-a cells after 5 days by ELISA
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[PMID: 20022495] |
Chemical Information
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CAS No. 75187-63-2
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Appearance Solid
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Molecular Weight 270.24
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Formula C15H10O5
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Color White to off-white
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SMILES
O=C1C(C2=CC=C(C=C2)O)=COC3=C1C=CC(O)=C3O
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Synonyms
7,8,4'-Trihydroxyisoflavone
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (185.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (246 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL | 92.5104 mL |
| 5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | 18.5021 mL | |
| 10 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL | 9.2510 mL | |
| 15 mM | 0.2467 mL | 1.2335 mL | 2.4669 mL | 6.1674 mL | |
| 20 mM | 0.1850 mL | 0.9251 mL | 1.8502 mL | 4.6255 mL | |
| 25 mM | 0.1480 mL | 0.7401 mL | 1.4802 mL | 3.7004 mL | |
| 30 mM | 0.1233 mL | 0.6167 mL | 1.2335 mL | 3.0837 mL | |
| 40 mM | 0.0925 mL | 0.4626 mL | 0.9251 mL | 2.3128 mL | |
| 50 mM | 0.0740 mL | 0.3700 mL | 0.7401 mL | 1.8502 mL | |
| 60 mM | 0.0617 mL | 0.3084 mL | 0.6167 mL | 1.5418 mL | |
| 80 mM | 0.0463 mL | 0.2313 mL | 0.4626 mL | 1.1564 mL | |
| 100 mM | 0.0370 mL | 0.1850 mL | 0.3700 mL | 0.9251 mL |
7,8-Dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one Related Classifications
HY-129409 Related Classifications
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