8-BuS-AMP 

8-BuS-AMP is a NTPDase1 inhibitor and a CD73/CD39 inhibitor, with an IC₅₀ of 35 μM and a K_i value of 0.292 μM against human NTPDase1; its K_i values against human CD73 and CD39 are 1.19 μM and 0.847 μM, respectively. 8-BuS-AMP binds to the substrate-binding pockets of NTPDase1 and CD73 to effectively block the conversion of ATP and AMP to adenosine, thereby enhancing the activation and proliferation of human peripheral T lymphocytes. 8-BuS-AMP possesses excellent enzymatic hydrolysis resistance and metabolic stability, resists hydrolysis by multiple NTPDase subtypes, and shows no activity against P2Y1 and P2Y12 receptors. 8-BuS-AMP can be used in purinergic signaling pathway and cancer-related studies^[1][2][3].

8-BuS-AMP (100 μM; 10 min) impairs recombinant human NTPDase1's ability to inhibit ADP-induced platelet aggregation, but does not itself induce or inhibit platelet aggregation, indicating in vitro inactivity towards platelet P2Y₁ and P2Y₁₂ receptors^[2].
8-BuS-AMP (1 μM; 0, 20, 40, 60 min) is highly metabolically stable in human and mouse liver microsomes, with half-lives of 462 min and 182 min, respectively^[3].
8-BuS-AMP (1-100 μM; 1 h) concentration-dependently inhibits nucleotide hydrolysis in human synovial fluid, reducing adenosine formation from both ε-ATP and ε-AMP^[3].
8-BuS-AMP (10-100 μM; 3 days, added daily) concentration-dependently enhances activation (via increased CD25 expression) and proliferation of human primary CD4⁺ and CD8⁺ T lymphocytes without reducing cell viability^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8-BuS-AMP (100 μM; added to histochemical reaction buffer) specifically inhibits NTPDase1-mediated ATPase activity in mouse liver vascular tissues without affecting NTPDase activity in kidney tubules^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

435.39

C₁₄H₂₂N₅O₇PS

344402-39-7

Solid

White to off-white

O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2SCCCC)O[C@@H]1COP(O)(O)=O

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Baqi Y, et al. Development of Anthraquinone Derivatives as Ectonucleoside Triphosphate Diphosphohydrolase (NTPDase) Inhibitors With Selectivity for NTPDase2 and NTPDase3. Front Pharmacol. 2020;11:1282. Published 2020 Aug 27.  [Content Brief]

  [2]. Lecka J, et al. 8-BuS-ATP derivatives as specific NTPDase1 inhibitors. Br J Pharmacol. 2013;169(1):179-196.  [Content Brief]

  [3]. Bi C, et al. Synthesis, Characterization, Interactions, and Immunomodulatory Function of Ectonucleotidase CD39/CD73 Inhibitor 8-Butylthioadenosine 5'-Monophosphate. ACS Pharmacol Transl Sci. 2025;8(5):1401-1415. Published 2025 Apr 3.  [Content Brief]