Cat. No.: HY-B0158

Cytidine

(Synonyms: Cytosine β-D-riboside; Cytosine-1-β-D-ribofuranoside)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Cytidine is a pyrimidine nucleoside and acts as a component of RNA. Cytidine is a precursor of uridine. Cytidine controls neuronal-glial glutamate cycling, affecting cerebral phospholipid metabolism, catecholamine synthesis, and mitochondrial function.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 65-46-3
Formula: C₉H₁₃N₃O₅
Molecular Weight:243.22

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Cytidine Chemical Structure

CAS No.: 65-46-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HOS-TE85	IC₅₀	2.6 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma MNNG-HOS cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma MNNG-HOS cell line	[PMID: 1895307]
KHOS-321H	IC₅₀	0.27 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma KHOS-321H cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma KHOS-321H cell line	[PMID: 1895307]
MKN-28	IC₅₀	4.5 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma MKN- 28 cell line Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma MKN- 28 cell line	[PMID: 1895307]
NUGC-4	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma NUGC-4 cell line Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma NUGC-4 cell line	[PMID: 1895307]
OST	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma OST cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma OST cell line	[PMID: 1895307]
PC-13	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-13 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-13 cell line	[PMID: 1895307]
PC-3	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-3 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-3 cell line	[PMID: 1895307]
PC-8	IC₅₀	0.28 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-8 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-8 cell line	[PMID: 1895307]
PC-9	IC₅₀	1.6 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-9 cell line Inhibitory effect tested in vitro for the growth of Lung adenocarcinoma PC-9 cell line	[PMID: 1895307]
QG-56	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Lung squamous-cell carcinoma QG-56 cell line Inhibitory effect tested in vitro for the growth of Lung squamous-cell carcinoma QG-56 cell line	[PMID: 1895307]
SK-ES1	IC₅₀	0.09 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Osteosarcoma SK-ES-1 cell line Inhibitory effect tested in vitro for the growth of Osteosarcoma SK-ES-1 cell line	[PMID: 1895307]
ST-KM-1	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma ST-KM cell line Inhibitory effect tested in vitro for the growth of Stomach adenocarcinoma ST-KM cell line	[PMID: 1895307]
SW480	IC₅₀	>100 μg/mL Compound: Cytidine	Inhibitory effect tested in vitro for the growth of colon adenocarcinoma SW-480 cell line Inhibitory effect tested in vitro for the growth of colon adenocarcinoma SW-480 cell line	[PMID: 1895307]

In Vivo

Cytidine decreases glutamate/glutamine levels and induces earlier improvement of depressive symptoms^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 65-46-3
Appearance Solid
Molecular Weight 243.22
Formula C₉H₁₃N₃O₅
Color White to off-white
SMILES

O[C@H]1[C@@H](O)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO
Synonyms

Cytosine β-D-riboside; Cytosine-1-β-D-ribofuranoside
Structure Classification
- Others
Initial Source
- Plants
- other families
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Animals
- Microorganisms
Widespread
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All RT-PCRCell Imaging/StainingCell Proliferation/Viability AssayCell Migration/Invasion Assay

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Journal Impact Factor

Imeta

2026 Feb 24.

Cytidine purchased from MedChemExpress. Usage Cited in: Imeta. 2026 Feb 24.

Cytidine (15 μM; 12 h) significantly increased the transcription of the viral gene 2A in EV-D68-infected RD cells.
Nat Mach Intell

2025 May.
Information Processing in Agriculture

2026 Mar 3.

Cytidine purchased from MedChemExpress. Usage Cited in: Information Processing in Agriculture. 2026 Mar 3.

Cytidine (200 μM; 24 h) significantly suppressed CYP19A1 expression in female gonadal cells.
Molecules

Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]

2023 Apr 11;28(8):3375. PMID: 37110608
J Mol Med (Berl)

Combination of PARP inhibitor and temozolomide to suppress chordoma progression. [Abstract]

2019 Aug;97(8):1183-1193. PMID: 31201471
Mol Med Rep

Causal association between metabolites and upper gastrointestinal tumors: A Mendelian randomization study. [Abstract]

2024 Dec;30(6):212. PMID: 39370813
J Pharm Biomed Anal

A novel UHPLC-MS/MS approach for simultaneous quantification of pyrimidine metabolites in human biofluids. [Abstract]

2025 Jun 17:265:117026. PMID: 40544701

Cytidine purchased from MedChemExpress. Usage Cited in: J Pharm Biomed Anal. 2025 Jun 17:265:117026. [Abstract]

Cytidine (50-100 µM; 72 h) inhibited the proliferation of both EC (KYSE150 cell) and GC (HGC27 cell) cell lines.

Cytidine purchased from MedChemExpress. Usage Cited in: J Pharm Biomed Anal. 2025 Jun 17:265:117026. [Abstract]

Cytidine (50-100 µM; 72 h) inhibited the migration of both EC (KYSE150 cell) and GC (HGC27 cell) cell lines.
University of Hawaii

2023 May.

Cytidine purchased from MedChemExpress. Usage Cited in: University of Hawaii. 2023 May.

Cytidine (205-410 µM; 4 d) significantly mitigated the negative effects of NHC on EBs.

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (205.58 mM)

DMSO : 50 mg/mL (205.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.1115 mL

20.5575 mL

41.1150 mL

5 mM

0.8223 mL

4.1115 mL

8.2230 mL

10 mM

0.4112 mL

2.0558 mL

4.1115 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (411.15 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jonas DA, et al. Safety considerations of DNA in food. Ann Nutr Metab. 2001;45(6):235-54. [Content Brief]

[2]. Wurtman RJ, et al. Effect of oral CDP-choline on plasma choline and uridine levels in humans. Biochem Pharmacol. 2000 Oct 1;60(7):989-92. [Content Brief]

[3]. Machado-Vieira R, et, al. New therapeutic targets for mood disorders. ScientificWorldJournal. 2010 Apr 13;10:713-26. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	4.1115 mL	20.5575 mL	41.1150 mL	102.7876 mL
	5 mM	0.8223 mL	4.1115 mL	8.2230 mL	20.5575 mL
	10 mM	0.4112 mL	2.0558 mL	4.1115 mL	10.2788 mL
	15 mM	0.2741 mL	1.3705 mL	2.7410 mL	6.8525 mL
	20 mM	0.2056 mL	1.0279 mL	2.0558 mL	5.1394 mL
	25 mM	0.1645 mL	0.8223 mL	1.6446 mL	4.1115 mL
	30 mM	0.1371 mL	0.6853 mL	1.3705 mL	3.4263 mL
	40 mM	0.1028 mL	0.5139 mL	1.0279 mL	2.5697 mL
	50 mM	0.0822 mL	0.4112 mL	0.8223 mL	2.0558 mL
	60 mM	0.0685 mL	0.3426 mL	0.6853 mL	1.7131 mL
	80 mM	0.0514 mL	0.2570 mL	0.5139 mL	1.2848 mL
	100 mM	0.0411 mL	0.2056 mL	0.4112 mL	1.0279 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cytidine

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Publications Citing Use of MedChemExpress (MCE) Cytidine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Cytidine Related Classifications

HY-B0158 Related Classifications