1. Metabolic Enzyme/Protease
  2. Glutaminase
  3. Glutaminase C-IN-1

Glutaminase C-IN-1  (Synonyms: Compound 968)

Cat. No.: HY-12682 Purity: 99.88%
COA Handling Instructions

Glutaminase C-IN-1 (Compound 968) is an allosteric inhibitor of Glutaminase C that inhibits cancer cell growth without affecting their normal cellular counterparts.

For research use only. We do not sell to patients.

Glutaminase C-IN-1 Chemical Structure

Glutaminase C-IN-1 Chemical Structure

CAS No. : 311795-38-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 119 In-stock
Solution
10 mM * 1 mL in DMSO USD 119 In-stock
Solid
5 mg USD 70 In-stock
10 mg USD 110 In-stock
50 mg USD 350 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Glutaminase C-IN-1 purchased from MedChemExpress. Usage Cited in: J Hepatol. 2020 May;72(5):909-923.  [Abstract]

    Proliferation of HCC-LM3 cells treated with Ctrl, 5 μM 968 alone, 4 μM Sor alone, and combined 5 μM 968 and 4 μM Sor (968+Sor).
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Glutaminase C-IN-1 (Compound 968) is an allosteric inhibitor of Glutaminase C that inhibits cancer cell growth without affecting their normal cellular counterparts[1][2].

    IC50 & Target

    Glutaminase C[2]

    In Vitro

    Glutaminase C-IN-1 (Compound 968) (10 μM; 14 d) inhibits cellular transformation in NIH 3T3 cells[1].
    ? Glutaminase C-IN-1 (10 μM; 6 d) blocks the signaling activity of a specific target of Dbl[1].
    ? Glutaminase C-IN-1 blocks the transforming activity of human breast cancer cells[1].
    ? Glutaminase C-IN-1 (10 μM) blocks glutaminolysis in transformed cells[2].
    ? Glutaminase C-IN-1 preferentially binds to the monomeric state of Glutaminase C[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: MDA-MB231 cells, SKBR3 cells, and NIH 3T3 cells
    Concentration: 10 μM
    Incubation Time: 5 h
    Result: Inhibited cell growth.
    In Vivo

    Glutaminase C-IN-1 (Compound 968) (200 μg/mouse; i.p.; daily for 12 days) reduces tumor volume in mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: SCID mice with P493 B lymphoma cells[1]
    Dosage: 200 μg/mouse
    Administration: Intraperitoneal injection, daily for 12 days
    Result: Caused a ~50% reduction in the size of the tumors.
    Molecular Weight

    475.42

    Formula

    C27H27BrN2O

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1C(C(C2=CC=C(N(C)C)C(Br)=C2)NC3=C4C5=CC=CC=C5C=C3)=C4CC(C)(C)C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (52.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1034 mL 10.5170 mL 21.0340 mL
    5 mM 0.4207 mL 2.1034 mL 4.2068 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months. When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (5.26 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (5.26 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.88%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months. When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1034 mL 10.5170 mL 21.0340 mL 52.5851 mL
    5 mM 0.4207 mL 2.1034 mL 4.2068 mL 10.5170 mL
    10 mM 0.2103 mL 1.0517 mL 2.1034 mL 5.2585 mL
    15 mM 0.1402 mL 0.7011 mL 1.4023 mL 3.5057 mL
    20 mM 0.1052 mL 0.5259 mL 1.0517 mL 2.6293 mL
    25 mM 0.0841 mL 0.4207 mL 0.8414 mL 2.1034 mL
    30 mM 0.0701 mL 0.3506 mL 0.7011 mL 1.7528 mL
    40 mM 0.0526 mL 0.2629 mL 0.5259 mL 1.3146 mL
    50 mM 0.0421 mL 0.2103 mL 0.4207 mL 1.0517 mL
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    Glutaminase C-IN-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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