6-Diazo-5-oxo-L-nor-Leucine
Based on 18 publication(s) in Google Scholar
6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) is a glutamine antagonist that irreversibly inhibits the catabolic effect of glutamine. 6-Diazo-5-oxo-L-nor-Leucine shows good anticancer activity (especially in pancreatic cancer) and reduces the self-renewal potential and metastatic capacity of tumour cells. 6-Diazo-5-oxo-L-nor-Leucine also possesses antibacterial and antiviral activity.
For research use only. We do not sell to patients.
- Purity : 99.98%
- CAS No.: 157-03-9
- Formula: C6H9N3O3
- Molecular Weight:171.15
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 6-Diazo-5-oxo-L-nor-Leucine
More- Signal Transduct Target Ther. 2026 Feb 19;11(1):64. [Abstract]
- Nature. 2026 Apr;652(8112):1339-1348. [Abstract]
- Cancer Discov. 2026 Apr 7. [Abstract]
- Redox Biol. 2021 Jul:43:101994. [Abstract]
- Adv Sci (Weinh). 2025 Jul;12(26):e2501815. [Abstract]
- Adv Sci (Weinh). 2025 Feb;12(6):e2411479. [Abstract]
- Adv Sci (Weinh). 2022 Oct;9(30):e2202993. [Abstract]
- Int J Biol Sci. 2022 Jun 21;18(10):4135-4150. [Abstract]
- Free Radic Biol Med. 2026 Oct:254:857-870.
- Clin Sci. 2025 Oct 22;139(20):1163-1185. [Abstract]
- Front Immunol. 2022 May 19:13:880262. [Abstract]
- J Agric Food Chem. 2026 Apr 15;74(14):11814-11831. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]
- J Biol Chem. 2026 May 28;302(7):113213. [Abstract]
- Cytokine. 2025 Jul:191:156943. [Abstract]
- Biochem Biophys Res Commun. 2022 Dec 17:634:1-9. [Abstract]
- bioRxiv. 2024 July 05.
- Sci Total Environ. 2023 Nov 1:897:165348. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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WB
All Antibiotic Isoforms
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Biological Activity
Description
IC50 & Target
Bacterial[3].
In Vitro
6-Diazo-5-oxo-L-nor-Leucine (DON) (0.3 mM; 1 h) shows inhibition of glutamine catabolism in WI-L2 cells[1].
6-Diazo-5-oxo-L-nor-Leucine (DON) decreases the selfrenewal potential and metastatic ability of tumor cell[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:WI-L2 cells
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Concentration:0.3 mM
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Incubation Time:1 h
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Result:Inhibited the rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 greater than 95%.
In Vivo
6-Diazo-5-oxo-L-nor-Leucine decreases hyaluronan and collagen in the tumor microenvironment, leading to an extensive remodeling of the ECM (extensive extracellular matrix), and an increases infiltration CD8+ T-cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 157-03-9
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Appearance Solid
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Molecular Weight 171.15
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Formula C6H9N3O3
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Color Off-white to light brown
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SMILES
N[C@@H](CCC(C=[N+]=[N-])=O)C(O)=O
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Synonyms
L-6-Diazo-5-oxonorleucine; DON
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (18)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
2026 Feb 19;11(1):64. PMID: 41708609 -
Nature
2026 Apr;652(8112):1339-1348. PMID: 41813904 -
Cancer Discov
Correcting Mitochondrial Complex I Defect in Tumor-Associated Natural Killer Cells Potentiates Immunotherapy for Glioblastoma. [Abstract]2026 Apr 7. PMID: 41944586 -
Redox Biol
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. [Abstract]2021 Jul:43:101994. PMID: 33964586 -
Adv Sci (Weinh)
2025 Jul;12(26):e2501815. PMID: 40270442 -
Adv Sci (Weinh)
Addressing Clinical Limitations of Glutaminase Inhibitors: Novel Strategies for Osimertinib-Resistant Lung Cancer by Exploiting Glutamine Metabolic Dependency. [Abstract]2025 Feb;12(6):e2411479. PMID: 39680480 -
Adv Sci (Weinh)
Bladder Cancer-Derived Small Extracellular Vesicles Promote Tumor Angiogenesis by Inducing HBP-Related Metabolic Reprogramming and SerRS O-GlcNAcylation in Endothelial Cells. [Abstract]2022 Oct;9(30):e2202993. PMID: 36045101 -
Int J Biol Sci
2022 Jun 21;18(10):4135-4150. PMID: 35844792 -
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Clin Sci
Glutamine alleviates immunosuppression in polymicrobial sepsis by augmenting bacterial phagocytosis through sustaining the GFAT-DRP1 dependent mitochondrial calcium dynamics. [Abstract]2025 Oct 22;139(20):1163-1185. PMID: 41082631 -
Front Immunol
Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation. [Abstract]2022 May 19:13:880262. PMID: 35663990
6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19:13:880262. [Abstract]
Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD4(+) T cells.
6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19:13:880262. [Abstract]
Splenocytes treated with 6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) has lower expressions of CD25 and CD69 in CD8(+) T cells.
6-Diazo-5-oxo-L-nor-Leucine purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19:13:880262. [Abstract]
6-Diazo-5-oxo-L-nor-Leucine (DON; 2.5 µM; 24 h) inhibits the the levels of phosphorylated mTOR and P70S6K (ConA-induced) in splenocytes.
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J Agric Food Chem
XBP1s-Regulated GFPT1 via Facilitating O-GlcNAcylation of FASN S509 Is Involved in Arsenic-Induced MASLD through Promoting Fatty Acid Synthesis in Hepatocytes. [Abstract]2026 Apr 15;74(14):11814-11831. PMID: 41920731 -
Acta Biochim Biophys Sin (Shanghai)
2023 Mar 20;55(8):1288-1300. PMID: 36942991 -
J Biol Chem
Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp. [Abstract]2026 May 28;302(7):113213. PMID: 42214671 -
Cytokine
Downregulation of GFPT2 enhances cisplatin chemotherapy sensitivity in STK11/KRAS mutant non-small cell lung cancer by regulating the hexosamine biosynthesis pathway, resisting tumor growth. [Abstract]2025 Jul:191:156943. PMID: 40253947 -
Biochem Biophys Res Commun
Glutaminase 1 blockade alleviates nonalcoholic steatohepatitis via promoting proline metabolism. [Abstract]2022 Dec 17:634:1-9. PMID: 36223657 -
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Sci Total Environ
Environmental cadmium exposure facilitates mammary tumorigenesis via reprogramming gut microbiota-mediated glutamine metabolism in MMTV-Erbb2 mice. [Abstract]2023 Nov 1:897:165348. PMID: 37429473
Solvent & Solubility
In Vitro:
H2O : 25 mg/mL (146.07 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Willis RC, et al. The inhibition by 6-diazo-5-oxo-l-norleucine of glutamine catabolism of the cultured human lymphoblast. J Cell Physiol. 1977 Dec;93(3):375-82. [Content Brief]
[2]. Sharma NS, et al. Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy. J Clin Invest. 2019 Oct 15. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.8428 mL | 29.2141 mL | 58.4283 mL | 146.0707 mL |
| 5 mM | 1.1686 mL | 5.8428 mL | 11.6857 mL | 29.2141 mL | |
| 10 mM | 0.5843 mL | 2.9214 mL | 5.8428 mL | 14.6071 mL | |
| 15 mM | 0.3895 mL | 1.9476 mL | 3.8952 mL | 9.7380 mL | |
| 20 mM | 0.2921 mL | 1.4607 mL | 2.9214 mL | 7.3035 mL | |
| 25 mM | 0.2337 mL | 1.1686 mL | 2.3371 mL | 5.8428 mL | |
| 30 mM | 0.1948 mL | 0.9738 mL | 1.9476 mL | 4.8690 mL | |
| 40 mM | 0.1461 mL | 0.7304 mL | 1.4607 mL | 3.6518 mL | |
| 50 mM | 0.1169 mL | 0.5843 mL | 1.1686 mL | 2.9214 mL | |
| 60 mM | 0.0974 mL | 0.4869 mL | 0.9738 mL | 2.4345 mL | |
| 80 mM | 0.0730 mL | 0.3652 mL | 0.7304 mL | 1.8259 mL | |
| 100 mM | 0.0584 mL | 0.2921 mL | 0.5843 mL | 1.4607 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
6-Diazo-5-oxo-L-nor-Leucine Related Classifications
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