Azaserine (Synonyms: CI-337; O-Diazoacetyl-L-serine; P-165)

Name: Azaserine
Brand: MedChemExpress
SKU: HY-B0919
Rating: 5.0 (5 reviews)

Cat. No.: HY-B0919 Purity: 99.57%: Data Sheet
COA

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Azazerine (CI-337) is a competitive inhibitor of glutamine amidotransferase. Azaserine is an antibiotic, it shows antibacterial activities. Azazerine shows anti-tumor activities and it may also act as a tumor inducer. Azazerine can be used for the research of cancer and infection.

For research use only. We do not sell to patients.

CAS No. : 115-02-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Azaserine:

Azaserine (Standard) Get quote

5 Publications Citing Use of MCE Azaserine

Cell Imaging/Staining

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Flow Cytometry

: Azaserine purchased from MedChemExpress. Usage Cited in: Vet Med Sci. 2025 Mar;11(2):e70294. [Abstract]; To study the effect of glucose on the development of GCs as mediated by GLUT, the glucose inhibitor Azaserine (5 µM) was added to GC cultures, which eliminated the FSH-induced glucose uptake capacity of the GCs.

: Azaserine purchased from MedChemExpress. Usage Cited in: Vet Med Sci. 2025 Mar;11(2):e70294. [Abstract]; Azaserine (5 μM) significantly reduced the viability of the GCs.

: Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]; Fluorescence images of BODIPY-C11-stained SW1990 pancreatic cancer cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or medium containing 2 mM glutamine supplemented with 50 μM diazooxonorleucine (Don) or 10 μM Azaserine (Aza). Cells treated with 10 μM RSL3 or 20 μM erastin were used as positive controls. Blue, DAPI; green, BODIPY-C11 (oxidized).

: Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]; Fluorescence images of BODIPY-C11-stained SU.8686 pancreatic cancer cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or medium containing 2 mM glutamine supplemented with 50 μM diazooxonorleucine (Don) or 10 μM Azaserine (Aza). Cells treated with 10 μM RSL3 or 20 μM erastin were used as positive controls. Blue, DAPI; green, BODIPY-C11 (oxidized).

: Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]; Proportion of BODIPY-C11 evaluated by flow cytometry in SU.8686 cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or media containing 2 mM glutamine supplemented with 50 μM Don or 10 μM Azaserine.

: Azaserine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2023 Mar 20;55(8):1288-1300. [Abstract]; Proportion of BODIPY-C11 evaluated by flow cytometry in SW1990 cells cultured for 48 h in glutamine-free medium, medium containing 2 mM glutamine, or media containing 2 mM glutamine supplemented with 50 μM Don or 10 μM Azaserine.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Azaserine (100 μg/mL; 6 h) causes some specific types of morphological changes of the sensitive organisms such as the characteristic elongation of Candida albicans cells^[2].
Azaserine (0-10 μM) inhibits the growth of E. coli strains with MIC values of 12.11, 51.9, 69.2 and 69.2 μg/mL for UTH 4, UTH 7036, UTH 7048 and UTH 7049^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Azaserine (5 mg/kg; i.p. once or twice a week for 6 months) induces tumor in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wistar rats^[1]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 5 mg/kg once or twice a week for 6 month
Result:	After 1 year most pancreases from treated rats were diffusely abnormal and contained many hyperplastic nodules and adenomas and more than rats one-quarter showed pancreatic adenocarcinoma.

Molecular Weight

173.13

Formula

C₅H₇N₃O₄

CAS No.

115-02-6

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](COC(C=[N+]=[N-])=O)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (288.80 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7760 mL	28.8800 mL	57.7601 mL
5 mM	1.1552 mL	5.7760 mL	11.5520 mL
10 mM	0.5776 mL	2.8880 mL	5.7760 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (273 KB) SDS (644 KB)

COA (244 KB) HNMR (178 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Longnecker DS, Curphey TJ. Adenocarcinoma of the pancreas in azaserine-treated rats. Cancer Res. 1975 Aug;35(8):2249-58. [Content Brief]

[2]. Shigemichi GUNJI, et al. Screening of Antifungal Antibiotics According to Activities Inducing Morphological Abnormalities. Agric. Biol Chem., 47 (9), 2061-2069, 1983.

[3]. Williams MV, Tritz GJ. Studies on the modes of action of azaserine inhibition of Escherichia coli. Potentiation of phenylalanine reversal. J Antimicrob Chemother. 1977 Jan;3(1):65-77. [Content Brief]

[4]. Catane R, et al. Azaserine, DON, and azotomycin: three diazo analogs of L-glutamine with clinical antitumor activity. Cancer Treat Rep. 1979 Jun;63(6):1033-8. [Content Brief]

[5]. Lyons SD, et al. Cytotoxic mechanisms of glutamine antagonists in mouse L1210 leukemia. J Biol Chem. 1990 Jul 5;265(19):11377-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.7760 mL	28.8800 mL	57.7601 mL	144.4002 mL
	5 mM	1.1552 mL	5.7760 mL	11.5520 mL	28.8800 mL
	10 mM	0.5776 mL	2.8880 mL	5.7760 mL	14.4400 mL
	15 mM	0.3851 mL	1.9253 mL	3.8507 mL	9.6267 mL
	20 mM	0.2888 mL	1.4440 mL	2.8880 mL	7.2200 mL
	25 mM	0.2310 mL	1.1552 mL	2.3104 mL	5.7760 mL
	30 mM	0.1925 mL	0.9627 mL	1.9253 mL	4.8133 mL
	40 mM	0.1444 mL	0.7220 mL	1.4440 mL	3.6100 mL
	50 mM	0.1155 mL	0.5776 mL	1.1552 mL	2.8880 mL
	60 mM	0.0963 mL	0.4813 mL	0.9627 mL	2.4067 mL
	80 mM	0.0722 mL	0.3610 mL	0.7220 mL	1.8050 mL
	100 mM	0.0578 mL	0.2888 mL	0.5776 mL	1.4440 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Azaserine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Azaserine (Synonyms: CI-337; O-Diazoacetyl-L-serine; P-165)

Customer Review

Other Forms of Azaserine:

Azaserine Related Antibodies

5 Publications Citing Use of MCE Azaserine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Azaserine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Azaserine Related Classifications

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Your Recently Viewed Products:

Customer Review

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