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OSMI-1 

Cat. No.: HY-119738
Handling Instructions

OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with an IC50 value of 2.7 μM. OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans.

For research use only. We do not sell to patients.

OSMI-1 Chemical Structure

OSMI-1 Chemical Structure

CAS No. : 1681056-61-0

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Description

OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with an IC50 value of 2.7 μM. OSMI-1 inhibits protein O-linked N-acetylglucosamine (O-GlcNAcylation) in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans[1][2].

IC50 & Target

IC50: 2.7 μM (O-GlcNAc transferase)[1]

In Vitro

OSMI-1 (50 μM; 24 hours; CHO cells) treatment decreases the viability by about 50% after 24 hours[1].
OSMI-1 (10-100 μM; 24 hours; CHO cells) treatment reduces global O-linked N-acetylglucosamine (O-GlcNAcylation) a dose-dependent manner. OSMI-1 inhibits OGT activity in cells[1].

Cell Viability Assay[1]

Cell Line: CHO cells
Concentration: 50 μM
Incubation Time: 24 hours
Result: Viability decreased by about 50% after 24 hours.

Western Blot Analysis[1]

Cell Line: CHO cells
Concentration: 10 μM, 25 μM, 50 μM, 100 μM
Incubation Time: 24 hours
Result: Reduced global OGlcNAcylation in a dose-dependent manner.
In Vivo

Mammalian and zebrafish toxicity profiles are strikingly similar, and zebrafish can usually serve as an intermediate step between cell-based evaluation and conventional animal testing. The zebrafish model is used to investigate OSM1-1 acute toxicity in vivo. The LC50 of OSM1-1 is 0.031 mg/mL (56 μM, 12 h) and 0.025 mg/mL (45 μM, 24 h) in zebrafish model[2].

Molecular Weight

563.64

Formula

C₂₈H₂₅N₃O₆S₂

CAS No.

1681056-61-0

SMILES

O=C(N(CC1=CC=CS1)CC2=CC=CO2)[[email protected]](NS(C3=CC(C=C4)=C(C=C3)NC4=O)(=O)=O)C5=CC=CC=C5OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

OSMI-1OSMI1OSMI 1OthersO-GlcNActransferaseOGTmammaliancell-permeableO-GlcNAcylationtoxicityzebrafishInhibitorinhibitorinhibit

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OSMI-1
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