Metamizole sodium hydrate
Based on 2 publication(s) in Google Scholar
Metamizole sodium hydrate (Dipyrone) is an orally active cyclooxygenase (COX) inhibitor.. Metamizole sodium hydrate can inhibit cell proliferation and promote cell apoptosis. Metamizole sodium hydrate has anti-inflammatory and antioxidant activities. Metamizole sodium hydrate is an antipyretic, analgesic and spasmolytic agent. Metamizole sodium hydrate can be used in research to relieve a variety of pain.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 5907-38-0
- Formula: C13H18N3NaO5S
- Molecular Weight:351.35
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Storage:
4°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Metamizole sodium hydrate
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Biological Activity
COX[1]
Metamizole sodium hydrate (1 and 10 μM, 24 h) can inhibit osteoblast differentiation and thus reduce its ability to generate new bone mineralized extracellular matrix[2].
Metamizole sodium hydrate (1-500 μM, 48 h) can inhibit cell proliferation and promote apoptosis in pancreatic cancer cell lines PaTu 8988 t and Panc-1[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PaTu 8988 t, Panc-1
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Concentration:1, 10, 100, 250, 500 μM
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Incubation Time:48 h
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Result:Inhibited proliferation in the PaTu 8988 t cell line with 1–500 μM and with concentrations of 1 μM, 10 μM, 100 μM, and 250 μM in the Panc-1 cell line. Increased the apoptosis rate after 9 h.
Metamizole sodium hydrate (250 or 500 mg/kg, orally) can effectively relieve pain and oxidative stress induced by scalpel incision and carrageenan in rats[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Neuropathic pain rat model [4]
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Dosage:500 mg/kg
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Administration:intraperitoneally preemptively at 16 and 1 h before Chronic constriction injury (CCI) and then twice a day for 7 days.
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Result:Decreased the expression of pronociceptive interleukins (IL-1beta, IL-6, and IL-18) and chemokines (CCL2, CCL4, and CCL7).
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Animal Model:Pain model in rats [5]
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Dosage:250 or 500 mg/kg
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Administration:p.o.
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Result:Decreased the COX-2 gene expression at 500 mg/kg and increased the MPO gene expression. Reduced MDA levels in the carrageenan paw test and increased tGSH levels.
Chemical Information
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CAS No. 5907-38-0
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Appearance Solid
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Molecular Weight 351.35
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Formula C13H18N3NaO5S
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Color White to off-white
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SMILES
O=S(CN(C1=C(C)N(C)N(C2=CC=CC=C2)C1=O)C)(O[Na])=O.O
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Synonyms
Dipyrone hydrate; Methamizole sodium hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Reticuline isomerase AKR1B1 with aldo-keto reductase activity and detoxification function from the insect Blaps rhynchopetera. [Abstract]2026 Mar:352:151224. PMID: 41791543 -
Animal Model Exp Med
Experimental study of a novel mouse model of tibial shaft fracture combined with blunt chest trauma. [Abstract]2025 Apr;8(4):696-706. PMID: 38225728
Solvent & Solubility
DMSO : 100 mg/mL (284.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (284.62 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (284.62 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Pierre SC, et al. Inhibition of cyclooxygenases by dipyrone. Br J Pharmacol. 2007 Jun;151(4):494-503. [Content Brief]
[2]. De Luna-Bertos E, et al. Therapeutic doses of nonsteroidal anti-inflammatory drugs inhibit osteosarcoma MG-63 osteoblast-like cells maturation, viability, and biomineralization potential. ScientificWorldJournal. 2013 Sep 19;2013:809891. [Content Brief]
[3]. Malsy M, et al. Effects of metamizole, MAA, and paracetamol on proliferation, apoptosis, and necrosis in the pancreatic cancer cell lines PaTu 8988 t and Panc-1. BMC Pharmacol Toxicol. 2017 Dec 6;18(1):77. [Content Brief]
[4]. Zajaczkowska R, et al. Metamizole relieves pain by influencing cytokine levels in dorsal root ganglia in a rat model of neuropathic pain. Pharmacol Rep. 2020 Oct;72(5):1310-1322. [Content Brief]
[5]. Ince I, Aksoy M, et al. A Comparative Investigation of the Analgesic Effects of Metamizole and Paracetamol in Rats. J Invest Surg. 2015 Jun;28(3):173-80. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 2.8462 mL | 14.2308 mL | 28.4616 mL | 71.1541 mL |
| 5 mM | 0.5692 mL | 2.8462 mL | 5.6923 mL | 14.2308 mL | |
| 10 mM | 0.2846 mL | 1.4231 mL | 2.8462 mL | 7.1154 mL | |
| 15 mM | 0.1897 mL | 0.9487 mL | 1.8974 mL | 4.7436 mL | |
| 20 mM | 0.1423 mL | 0.7115 mL | 1.4231 mL | 3.5577 mL | |
| 25 mM | 0.1138 mL | 0.5692 mL | 1.1385 mL | 2.8462 mL | |
| 30 mM | 0.0949 mL | 0.4744 mL | 0.9487 mL | 2.3718 mL | |
| 40 mM | 0.0712 mL | 0.3558 mL | 0.7115 mL | 1.7789 mL | |
| 50 mM | 0.0569 mL | 0.2846 mL | 0.5692 mL | 1.4231 mL | |
| 60 mM | 0.0474 mL | 0.2372 mL | 0.4744 mL | 1.1859 mL | |
| 80 mM | 0.0356 mL | 0.1779 mL | 0.3558 mL | 0.8894 mL | |
| 100 mM | 0.0285 mL | 0.1423 mL | 0.2846 mL | 0.7115 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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