NVP-LCQ195
Based on 1 Customer Validation
NVP-LCQ195 (LCQ-195) is an inhibitor of CDK1/2/3/5/9. NVP-LCQ195 induces cell cycle arrest and eventual apoptotic cell death of multiple myeloma (MM) cells. NVP-LCQ195 triggers decreased amplitude of transcriptional signatures related to oncogenesis, drug resistance and stem cell renewal in MM cell lines.
For research use only. We do not sell to patients.
- Purity: 98.88%
- CAS No.: 902156-99-4
- Formula: C17H19Cl2N5O4S
- Molecular Weight:460.33
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cdk5/p25 1 nM (IC50) |
CDK5/p35 1 nM (IC50) |
Cdk1/cyclin B 2 nM (IC50) |
cdk2/cyclin A 2 nM (IC50) |
CDK2/cyclinE 5 nM (IC50) |
CDK9/cyclinT1 15 nM (IC50) |
CDK3/Cyclin E 42 nM (IC50) |
cdk6/cyclin D3 187 nM (IC50) |
CDK7/Cyclin H/MAT1 3564 nM (IC50) |
NVP-LCQ195 (Compound LCQ-195) (500 nmol/L) blocks the activity of CDK1/cyclin B and CDK2/cyclin A, as well as CDK5 in MM cell lines[1].
NVP-LCQ195 (0-4 μmol/L, 48 h) results in the EC50 of equal to or less than 1 μmol/L in most MM cell lines[1].
NVP-LCQ195 (2 μmol/L, 4-48 h) increases the percentage of cells in S and G2/M phases early, followed by an increase in sub-G1 population[1].
NVP-LCQ195 (2 μmol/L, 2-24 h) decreases the amplitude of various transcriptional signatures indicative of the activity of transcription factors (e.g. myc, HIF-1α, IRF4 and NF-κB), signalling cascades (e.g. Notch, Ras, Akt and Hedgehog), the ubiquitin/proteasome and ribosomal pathways in MM cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 902156-99-4
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Appearance Solid
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Molecular Weight 460.33
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Formula C17H19Cl2N5O4S
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Color White to off-white
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SMILES
CS(=O)(N1CCC(NC(C2=C(NC(C3=C(Cl)C=CC=C3Cl)=O)C=NN2)=O)CC1)=O
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Synonyms
LCQ-195; AT9311
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 66.67 mg/mL (144.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1724 mL | 10.8618 mL | 21.7235 mL | 54.3089 mL |
| 5 mM | 0.4345 mL | 2.1724 mL | 4.3447 mL | 10.8618 mL | |
| 10 mM | 0.2172 mL | 1.0862 mL | 2.1724 mL | 5.4309 mL | |
| 15 mM | 0.1448 mL | 0.7241 mL | 1.4482 mL | 3.6206 mL | |
| 20 mM | 0.1086 mL | 0.5431 mL | 1.0862 mL | 2.7154 mL | |
| 25 mM | 0.0869 mL | 0.4345 mL | 0.8689 mL | 2.1724 mL | |
| 30 mM | 0.0724 mL | 0.3621 mL | 0.7241 mL | 1.8103 mL | |
| 40 mM | 0.0543 mL | 0.2715 mL | 0.5431 mL | 1.3577 mL | |
| 50 mM | 0.0434 mL | 0.2172 mL | 0.4345 mL | 1.0862 mL | |
| 60 mM | 0.0362 mL | 0.1810 mL | 0.3621 mL | 0.9051 mL | |
| 80 mM | 0.0272 mL | 0.1358 mL | 0.2715 mL | 0.6789 mL | |
| 100 mM | 0.0217 mL | 0.1086 mL | 0.2172 mL | 0.5431 mL |
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