1. Cell Cycle/DNA Damage Epigenetics PI3K/Akt/mTOR
  2. HDAC mTOR
  3. Pomiferin

Pomiferin (NSC 5113) acts as an potential inhibitor of HDAC, with an IC50 of 1.05 μM, and also potently inhibits mTOR (IC50, 6.2 µM).

For research use only. We do not sell to patients.

Pomiferin Chemical Structure

Pomiferin Chemical Structure

CAS No. : 572-03-2

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Pomiferin (NSC 5113) acts as an potential inhibitor of HDAC, with an IC50 of 1.05 μM, and also potently inhibits mTOR (IC50, 6.2 µM).

IC50 & Target[1][2]

HDAC

1.05 μM (IC50)

mTOR

6.2 μM (IC50)

In Vitro

Pomiferin is an potential inhibitor of HDAC, with an IC50 of 1.05 μM. Pomiferin shows cytotoxic effects on human tumor cell lines, with GI50s of 1.32 ± 0.02 μM (HCT-15 cells), 2.92 ± 0.09 μM (MDA-MB-231 cells), 3.18 ± 0.05 μM (ACHN cells), 3.34 ± 0.11 μM (LOX-IMVI cells), 3.95 ± 0.05 μM (PC-3 cells), 5.14 ± 0.06 μM (NCI-H23 cells), and 123 μM (Hepatocyte cells)[1]. Pomiferin is a highly specific mTOR inhibitor, with an IC50 of 6.2 µM. Pomiferin triacetate only affects two PI3Kα mutants, E542K and E545K. Pomiferin triacetate (0.3125-20 µM) stabilizes Pdcd4 from TPA-induced degradation in HEK293 cells. Pomiferin triacetate (20 µM) inhibits IGF-1-induced signaling downstream of Akt activation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pomiferin (5, 10 and 20 mg/kg, p.o.) shows protective effects on the treatment of reperfusion injury. Pomiferin also increases SOD activities and total antioxidative capacity, and decreases malondialdehyde in rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

420.45

Formula

C25H24O6

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=C(O)C(C/C=C(C)\C)=C3C(C=CC(C)(C)O3)=C2OC=C1C4=CC=C(O)C(O)=C4

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation
References
Cell Assay
[1]

Cell lines purchased from ATCC are maintained in Dulbecco’s modified Eagle’s media (DMEM) supplemented with 10% horse serum and 5% fetal bovine serum and incubated in a CO2 incubator (5%) at 37°C. Cells are serum-deprived by three washes of PBS and resuspended in DMEM. The suspended cells are plated on 96-well plates (1 × 104 cells/well) and treated with Pomiferin. After treatment for 21 h, MTT is added to the medium (0.5 mg/mL), and the mixture is incubated at 37°C for another 3 h. After discarding the medium, DMSO (100 mL) is then applied to the well to dissolve the formazan crystals, and the absorbances at 570 and 630 nm in each well are measured on a micro-ELISA reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Rats[3]
Male Wistar SPF laboratory rats are used in the study. After 10 days of acclimation, 50 animals are randomised in 5 groups (n=10). One group is left intact. Four groups undergo laparotomy in general anaesthesia (2% Rometar (xylazine) 0.5 mL + Narkamon (ketamine) 10 mL, dose 0.5 mL solution /100 g of body mass). Ischaemia-reperfusion injury is induced by applying a vascular clamp on the left renal artery for 60 min with subsequent renal reperfusion. Each animal (including those of the intact group) is put into its own metabolic cage. For 15 days all the animals are bred in these cages. The doses of pomiferin are suspended in 2 mL of 0.5% Avicel solution (microcrystalline cellulose) and are administered starting with day 1 after operation. Three of four operated groups are medicated with Pomiferin-orally by gastric gavage once a day in different doses: 5 mg/kg, 10 mg/kg and 20 mg/kg. The placebo group is given 2 mL of 0.5% Avicel by the same route. On day 15, all the animals are exsanguinated in general anaesthesia (2% Rometar 0.5 mL + Narkamon 10 mL, dose 0.5 mL solution /100 g of the rat body mass) by blood collection from the left ventricle and the reperfused kidney tissue is employed for histopathological examination[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Pomiferin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Pomiferin
Cat. No.:
HY-N4315
Quantity:
MCE Japan Authorized Agent: