Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1)
Based on 1 Customer Validation
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) is a rat-derived IgG2b κ type antibody inhibito, targeting to mouse CD122/IL-2Rβ. Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) can block IL-2 and IL-15. Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) can deplete natural killer (NK) cells and NKT cells. Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) can be used for the researches of cancer, infection, inflammation, immunology and metabolic disease, such as melanoma, experimental autoimmune encephalomyelitis (EAE) and diabetes.
For research use only. We do not sell to patients.
- Purity : 95%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
Rat IgG2b kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
CD122/IL-2Rβ
In Vivo
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (200 μg, i.p., weekly from 5-15 weeks of age) decreases the frequency of mice that developed diabetes and delays the disease onset in not obese diabetic mice models[2].
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (500 μg, i.p., before and 4 days after infection) depletes natural killer (NK) cells and NKT cells in Neospora caninum infection mice models[3].
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (300 μg/mouse, i.p., at days 6 and 7 after transplantation) abrogates the expansion advantage of OT-I tumor-infiltrating T lymphocytes companied with anti-CD132 (HY-P990292) in Ptgs1/Ptgs2−/−BRAFV600E melanoma mice models[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MOG-induced experimental autoimmune encephalomyelitis (EAE) mice models[1]
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Dosage:50 μg
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Administration:Intraperitoneally injection, 1 day before induction
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Result:Showed earlier initiation of EAE symptoms with high clinical scores from day 9 until day 40.
Reduced numbers of NK and CD8+CD122+ T cells.
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Animal Model:Not obese diabetic mice models[2]
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Dosage:200 μg
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Administration:Intraperitoneally injection, weekly from 5-15 weeks of age
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Result:Reduced NK cells from the spleen, pancreas and PLN.
Reduced CD8+ T cells in the pancreas.
Reduced CD8+CD122+ T cells in the spleen.
Abolished NK cell function but not T cell.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vivo NK cell depletion; in vivo CD122 blockade; in vitro IL-2R blockade; Functional assays; F
Verified Bioactivity
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Flow cytometric analysis of 1×106 Jurkat cells labelling CD122 (red) with Anti-Mouse CD122 Antibody (HY-P990808). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2b kappa Isotype Control (HY-P990682, blue) was used as the isotype control.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD122/IL-2Rb Antibody (TM-Beta 1)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yu P, et al. IL-15-dependent CD8+ CD122+ T cells ameliorate experimental autoimmune encephalomyelitis by modulating IL-17 production by CD4+ T cells. Eur J Immunol. 2014 Nov;44(11):3330-41. [Content Brief]
[2]. Brauner H, et al. Depletion of IL-2 receptor β-positive cells protects from diabetes in non-obese diabetic mice. Immunol Cell Biol. 2016 Feb;94(2):177-84. [Content Brief]
[3]. Nishikawa Y, et al. Roles of CD122+ cells in resistance against Neospora caninum infection in a murine model. J Vet Med Sci. 2010 Oct;72(10):1275-82. [Content Brief]
[4]. Lacher SB, et al. PGE2 limits effector expansion of tumour-infiltrating stem-like CD8+ T cells. Nature. 2024 May;629(8011):417-425. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)