Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) 

Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) is a rat-derived IgG2b κ type antibody inhibito, targeting to mouse CD122/IL-2Rβ. Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) can block IL-2 and IL-15. Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) can deplete natural killer (NK) cells and NKT cells. Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) can be used for the researches of cancer, infection, inflammation, immunology and metabolic disease, such as melanoma, experimental autoimmune encephalomyelitis (EAE) and diabetes^[1][2][3][4].

Rat IgG2b kappa

Rat IgG2b kappa, Isotype Control

Mouse

CD122/IL-2Rβ

Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (50 μg, i.p., 1 day before induction) blocks IL-15 and aggravates EAE severity in MOG-induced experimental autoimmune encephalomyelitis (EAE) mice models^[1].
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (200 μg, i.p., weekly from 5-15 weeks of age) decreases the frequency of mice that developed diabetes and delays the disease onset in not obese diabetic mice models^[2].
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (500 μg, i.p., before and 4 days after infection) depletes natural killer (NK) cells and NKT cells in Neospora caninum infection mice models^[3].
Anti-Mouse CD122/IL-2Rβ Antibody (TM-Beta 1) (300 μg/mouse, i.p., at days 6 and 7 after transplantation) abrogates the expansion advantage of OT-I tumor-infiltrating T lymphocytes companied with anti-CD132 (HY-P990292) in Ptgs1/Ptgs2^−/−BRAF^V600E melanoma mice models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

16185  [NCBI]

P16297

in vivo NK cell depletion; in vivo CD122 blockade; in vitro IL-2R blockade; Functional assays; F

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse CD122/IL-2Rb Antibody (TM-Beta 1)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1×10⁶ Jurkat cells labelling CD122 (red) with Anti-Mouse CD122 Antibody (HY-P990808). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2b kappa Isotype Control (HY-P990682, blue) was used as the isotype control.

• [1]. Yu P, et al. IL-15-dependent CD8+ CD122+ T cells ameliorate experimental autoimmune encephalomyelitis by modulating IL-17 production by CD4+ T cells. Eur J Immunol. 2014 Nov;44(11):3330-41.  [Content Brief]

  [2]. Brauner H, et al. Depletion of IL-2 receptor β-positive cells protects from diabetes in non-obese diabetic mice. Immunol Cell Biol. 2016 Feb;94(2):177-84.  [Content Brief]

  [3]. Nishikawa Y, et al. Roles of CD122+ cells in resistance against Neospora caninum infection in a murine model. J Vet Med Sci. 2010 Oct;72(10):1275-82.  [Content Brief]

  [4]. Lacher SB, et al. PGE2 limits effector expansion of tumour-infiltrating stem-like CD8+ T cells. Nature. 2024 May;629(8011):417-425.  [Content Brief]