Copy Link Print Information

Calpain 1 Antibody (YA815)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Calpain 1 Antibody (YA815) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to Calpain 1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgM

  • Application:

    ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
  • Calpain 1 Antibody (YA815) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Calpain 1 Antibody (YA815)

More

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:50-1:200 1:50-1:200 1:50-1:100

Product Details

Description

Calpain 1 Antibody (YA815) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to Calpain 1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 75/80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 82 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Calpain 1.AA range:1-714.

Sensitivity

Endogenous

Purification

Protein L affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgM

RRID

AB_3102902

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Calpain 1 Antibody (YA815)
    Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
    Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Calpain 1 is a Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7)[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:general expression

  • Subunit

    Forms a heterodimer with a small (regulatory) subunit CAPNS1

  • SwissProt ID

    P07384

  • Gene ID
    823 [NCBI]
  • Research Field

    Signal Transduction

Calpain 1 Antibody (YA815) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Calpain 1 Antibody (YA815) Product Image
    1/16
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
  • Calpain 1 Antibody (YA815) Product Image
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
  • Experimental Validation Results for Calpain 1 Antibody (YA815)
  • Calpain 1 Antibody (YA815) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Calpain 1 Antibody (YA815) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Calpain 1 Antibody (YA815) Product Image ICC
  • Calpain 1 Antibody (YA815) Product Image ICC