Calpain 1 Antibody (YA815)

Cat. No.: HY-P80443: Data Sheet COA
SDS
User Guide for Antibodies
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Calpain 1 Antibody (YA815) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to Calpain 1.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
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Calpain 1 Antibody (YA815) Featured Recommendations:

1 Publications Citing Use of MCE Calpain 1 Antibody (YA815)

•Adv Sci (Weinh). 2025 Apr 2:e2501112. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Calpain 1 Antibody (YA815) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to Calpain 1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 82 kDa;

Observed band size: 75/80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P07384

Gene ID

823 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Calpain 1.AA range:1-714.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Protein L affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgM

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:19617626, PubMed:21531719, PubMed:2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed:23707407). Cleaves and activates caspase-7 (CASP7) (PubMed:19617626)

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:general expression

Subunit：Forms a heterodimer with a small (regulatory) subunit CAPNS1

RRID

AB_3102902

Database

Entrez Gene: 823 Human ; 12333 Mouse ; 29153 Rat

SwissProt: P07384 Human ; O35350 Mouse ; P97571 Rat

OMIM: 616907 Human

Research Field

Signal Transduction

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Calpain 1 Antibody (YA815) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress Validation
Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
1/16

MedChemExpress Validation
Immunocytochemistry analysis of Siha cells labeling Calpain 1 with Calpain 1 Antibody (HY-P80443) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calpain 1 Antibody (HY-P80443) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
1/16

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Calpain 1 Antibody (YA815)

Calpain 1 Antibody (YA815) Featured Recommendations:

1 Publications Citing Use of MCE Calpain 1 Antibody (YA815)

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Background

Documentation

Calpain 1 Antibody (YA815) Related Classifications

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