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GPCR RDC1/CXCR-7 Antibody (YA3907)

(Synonyms: RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159)
Customer Review

Based on 1 Customer Validation

GPCR RDC1/CXCR-7 Antibody (YA3907) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GPCR RDC1/CXCR-7.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL FC ICC
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:50-1:200 1:200-1:400 1:10000

Product Details

Description

GPCR RDC1/CXCR-7 Antibody (YA3907) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GPCR RDC1/CXCR-7.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 41 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Immunogen

Purified recombinant fragment of human CXCR7(AA: extra mix) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling CXCR7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
    Immunofluorescence analysis of Hela cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
    Immunofluorescence analysis of NIH3T3 cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    GPCR RDC1/CXCR-7 is an Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels; (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Early endosome; Recycling endosome

  • Expression


    Tissue_specificity:It is expressed in monocytes, basophils, B cells, umbilical vein endothelial cells (HUVECs) , and B lymphoblasts. It is expressed at lower levels in CD4+ T lymphocytes and natural killer cells. In brain tissue, it can be detected in endothelial cells and capillaries, as well as in mature neurons of the frontal cortex and hippocampus. It is expressed during renal tubule formation in the kidneys. It is highly expressed in astrocytoma endothelial cells, microglia, and glioma cells. It is expressed at low levels in normal CD34+ progenitor cells but at high levels in several myeloid malignant cell lines. It is expressed in breast cancer but not in normal breast tissue (protein level) .

    Induction:Up-regulated during cell differentiation in glioma cells

  • Subunit

    Homodimer. Can form heterodimers with CXCR4; heterodimerization may regulate CXCR4 signaling activity. Interacts with ARRB1 and ARRB2

  • SwissProt ID

    P25106

  • Gene ID
    57007 [NCBI]
  • Synonyms

    RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159

GPCR RDC1/CXCR-7 Antibody (YA3907) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling CXCR7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of NIH3T3 cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image
    1/16
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling CXCR7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of NIH3T3 cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • Experimental Validation Results for GPCR RDC1/CXCR-7 Antibody (YA3907)
  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling CXCR7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of NIH3T3 cells labeling CXCR7 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CXCR7 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image FC
  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image ICC
  • GPCR RDC1/CXCR-7 Antibody (YA3907) Product Image ICC