F4/80 Antibody (YA002)

MedChemExpress Validation
Flow cytometric analysis of 1X10⁶ RAW264.7 cells labeling F4/80 Antibody (HY-P80665, red). Cells were stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rat IgG H&L (HY-P89199) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rat IgG2a Isotype Control (HY-P89510, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

F4/80 Antibody (YA002) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Jul 1:180:116695. [Abstract]

Customer Validation
Immunofluorescence images for NLRP3 and F4/80 in adipose tissue (scale 100 μm). F4/80 Antibody (YA002) (F4/80) was used as a marker for macrophages. Immunofluorescence for NLRP3 co-stained with F4/80 indicated a higher average expression of NLRP3 in ATMs for obesity-related severe acute pancreatitis (SAP).

F4/80 Antibody (YA002) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Jul 1:180:116695. [Abstract]

Customer Validation
Immunofluorescence images for Caspase1 and F4/80 in adipose tissue (scale 100 μm). F4/80 Antibody (YA002) (F4/80) was used as a marker for macrophages. Immunofluorescence for Caspase1 co-stained with F4/80 indicated a higher average expression of Caspase1 in ATMs for obesity-related severe acute pancreatitis (SAP).

F4/80 Antibody (YA002) purchased from MedChemExpress. Usage Cited in: Cells. 2025 Sep 16;14(18):1452. [Abstract]

Customer Validation
Representative immunohistochemical staining and quantification of the macrophage marker F4/80 in myocardial tissue sections. Scale bars for whole heart sections are 0.25 mm, and for magnified views are 20 µm. Black arrows indicate F4/80 positive macrophages. Sections were blocked with 5% goat serum and incubated overnight at 4 °C with the following primary antibodies: anti-α-smooth muscle actin (α-SMA) (HY-P80484, 1:200), anti-F4/80 (F4/80 Antibody (YA002), HY-P80665, 1:200), anti-Integrin α5 (ITGA5) (HY-P80416, 1:200), and anti-Desmocollin-2 (DSC2) (1:200). After incubation with appropriate HRP-conjugated secondary antibodies, signals were visualized using a DAB substrate kit.

F4/80 Antibody (YA002) purchased from MedChemExpress. Usage Cited in: iMetaMed. 2025 Oct 21.

Customer Validation
Co-immunofluorescence staining of CLEC4E (red) and F4/80 (green) in colonic tissues (scale bar: 100 µm). 5 μm-thick frozen colonic tissue slides and pre-fixed cell samples were incubated with 5% BSA in PBS at room temperature for 1.5 h to block nonspecific binding. Then the above samples were exposed to primary antibodies specific for CLEC4E (1:200), F4/80 Antibody (YA002) (1:100) overnight in a 4°C refrigerator. After that, the specimens were washed three times in PBS and incubated with fluorescently labeled secondary antibodies for 1.5 h at room temperature under dark conditions. Following further PBS rinses, nuclear staining was performed with DAPI, and confocal fluorescence images were collected using a Leica microscope.

F4/80 Antibody (YA002) purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177000. [Abstract]

Customer Validation
Representative immunofluorescence staining images of iNOS and Arg-1 in RAW 264.7 cells and the quantification analysis of iNOS and Arg-1 expression. To investigate whether NVP-BHG712 affects macrophage polarization, macrophages were labeled with anti-F4/80 (F4/80 Antibody (YA002), overnight at 4 °C) antibody, while M1 and M2 macrophages were labeled with anti-iNOS and anti-Arg-1 antibodies, respectively. Immunofluorescence staining results showed that LPS stimulation enhanced polarization toward the iNOS+ M1 phenotype and significantly inhibited polarization toward the Arg-1+ M2 phenotype.