FAP Antibody (YA1442)

Cat. No.: HY-P81697: Ficha de datos COA
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FAP Antibody (YA1442) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FAP.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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FAP Antibody (YA1442) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

FAP Antibody (YA1442) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FAP.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 88 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q12884

Gene ID

2191 [NCBI]

Immunogen

Purified recombinant fragment of human FAP(aa26-264) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
FC FC: Flow Cytometry	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Pureza	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16410248, PubMed:16480718, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711, PubMed:2172980, PubMed:7923219, PubMed:9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16410248, PubMed:16651416, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner

Subcellular Localization：Cell surface; Cell membrane; Single-pass type II membrane protein; Cell projection, lamellipodium membrane; Single-pass type II membrane protein; Cell projection, invadopodium membrane; Single-pass type II membrane protein; Cell projection, ruffle membrane; Single-pass type II membrane protein; Membrane; Single-pass type II membrane protein; Secreted; Cytoplasm

Expression：
Tissue_specificity:Expressed in adipose tissue. Expressed in dermal fibroblasts of fetal skin. Expressed in granulation tissue of healing wounds and reactive stromal fibroblasts of epithelial carcinoma. Expressed in activated fibroblast-like synovial cells in inflamed synovial tissue. Expressed in activated hepatic stellate cells (HSCs) and myofibroblasts in cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (protein level) . Expressed in glioblastoma and glioma cells. Isomer 1 and isomer 2 are expressed in melanoma, carcinoma, and fibroblast cell lines.

Induction:In fibroblasts at times and sites of tissue remodeling during development, tissue repair and carcinogenesis. Up-regulated upon tumor stem cell differentiation. Up-regulated by transforming growth factor-beta, 12-O-tetradecanoyl phorbol-13-acetate and retinoids

Isoforms & Post-Translational Modification：Q12884 has 2 isomers: Q12884-1: 87713 Da (predicted); Q12884-2: 26954 Da (predicted).
N-glycosylated;The N-terminus may be blocked

Subunit：Homodimer; homodimerization is required for activity of both plasma membrane and soluble forms. The monomer is inactive. Heterodimer with DPP4. Interacts with PLAUR; the interaction occurs at the cell surface of invadopodia membranes. Interacts with ITGB1. Interacts with ITGA3. Associates with integrin alpha-3/beta-1; the association occurs in a collagen-dependent manner at the cell surface of invadopodia membranes

RRID

AB_3103804

Database

Entrez Gene: 2191 Human

SwissProt: Q12884 Human

OMIM: 600403 Human

Research Field

Cell Biology

Synonyms

FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

FAP Antibody (YA1442) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FAP Antibody (YA1442)

FAP Antibody (YA1442) Recomendaciones destacadas:

Product Detail

Background

Descripciòn

FAP Antibody (YA1442) Related Classifications

Productos vistos recientemente:

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