FOXM1 Antibody (YA4205)(PBS only)

Cat. No.: HY-P84508A: Data Sheet User Guide for Antibodies
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FOXM1 Antibody (YA4205) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXM1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FOXM1 Antibody (YA4205) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXM1.

Host

Mouse

Molecular Weight

Predicted band size: 84 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q08050

Gene ID

2305 [NCBI]

Immunogen

Purified recombinant fragment of human FOXM1 (AA: 649-748) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed:19160488, PubMed:20360045). Plays a role in the control of cell proliferation (PubMed:19160488). Also plays a role in DNA break repair, participating in the DNA damage checkpoint response (PubMed:17101782). Promotes transcription of PHB2 (PubMed:33754036)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:This gene is expressed in the thymus, testes, small intestine, colon, and ovary. It appears to be expressed only in adult organs containing proliferating/cycled cells, or in response to growth factor stimulation. It is also expressed in tumor-derived epithelial cell lines. It is not expressed in quiescent cells. Isomer 2 is highly expressed in the testes.

Induction:Induced during liver regeneration and oxidative stress

Isoforms & Post-Translational Modification：Q08050 has 3 isomers: Q08050-1: 84283 Da (predicted); Q08050-2: 82685 Da (predicted); Q08050-3: 88596 Da (predicted).
Phosphorylated in M (mitotic) phase (PubMed:17101782, PubMed:19160488, PubMed:30139873). Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair (PubMed:17101782). Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1 (PubMed:19160488, PubMed:30139873). Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators (PubMed:19160488). Phosphorylated by GSK3B leading to ubiquitination and proteasomal degradation (PubMed:26912724);Ubiquitinated in a FBXW7-dependent manner leading to proteasomal degradation

Subunit：Interacts with PINT87aa which is encoded by the circular form of the long non-coding RNA LINC-PINT; the interaction inhibits FOXM1-mediated transcription of PHB2

Synonyms

MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2

Documentation

User Guide for Antibodies (1077 KB)