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PTPN14 Antibody (YA4294)

(Synonyms: PEZ; PTP36)
Customer Review

Based on 1 Customer Validation

PTPN14 Antibody (YA4294) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PTPN14.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P FC ICC
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • PTPN14 Antibody (YA4294) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

PTPN14 Antibody (YA4294) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PTPN14.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 135 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 135.2 kDa
Immunogen

Purified recombinant fragment of human PTPN14 (AA: 896-1169) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718945

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human breast cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Flow cytometric analysis of 1X10^6 Hela cells labeling PTPN14 Antibody(HY-P84597
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunocytochemistry analysis of HeLa cells labeling PTPN14 Antibody (HY-P84597) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PTPN14 Antibody (HY-P84597) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    Immunofluorescence analysis of LOVO cells labeling PTPN14 antibody (HY-P84597) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

Background

  • Function

    PTPN14 is a Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton; Nucleus

  • Expression


    Tissue_specificity:general expression

    Induction:Up-regulated at protein level by cell density. However, at the mRNA level remains the same regardless of the status of cell density

  • Subunit

    Interacts with FLT4; the interaction is enhanced by stimulation with VEGFC. Interacts (via PPxY motifs) with YAP1 (via WW domains); this interaction leads to the cytoplasmic sequestration of YAP1 and inhibits its transcriptional co-activator activity

  • SwissProt ID

    Q15678

  • Gene ID
    5784 [NCBI]
  • Synonyms

    PEZ; PTP36

PTPN14 Antibody (YA4294) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling PTPN14 Antibody(HY-P84597

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PTPN14 Antibody (HY-P84597) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PTPN14 Antibody (HY-P84597) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of LOVO cells labeling PTPN14 antibody (HY-P84597) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • PTPN14 Antibody (YA4294) Product Image
    1/16
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • PTPN14 Antibody (YA4294) Product Image
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling PTPN14 Antibody(HY-P84597
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PTPN14 Antibody (HY-P84597) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PTPN14 Antibody (HY-P84597) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of LOVO cells labeling PTPN14 antibody (HY-P84597) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • Experimental Validation Results for PTPN14 Antibody (YA4294)
  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using PTPN14 Antibody (HY-P84597, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using PTPN14 Antibody (HY-P84597, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling PTPN14 Antibody(HY-P84597

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling PTPN14 Antibody (HY-P84597) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PTPN14 Antibody (HY-P84597) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PTPN14 Antibody (YA4294) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of LOVO cells labeling PTPN14 antibody (HY-P84597) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image IHC-P
  • PTPN14 Antibody (YA4294) Product Image FC
  • PTPN14 Antibody (YA4294) Product Image ICC
  • PTPN14 Antibody (YA4294) Product Image ICC