SIRT4 Antibody (YA4600)(PBS only)
(Synonyms: SIR2L4)SIRT4 Antibody (YA4600) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIRT4.
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Host:
Mouse
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Isotype:
IgG
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Application:
ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
SIRT4 Antibody (YA4600) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIRT4.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Purified recombinant fragment of human SIRT4 (AA: 215-314) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SIRT4 is a mitochondrial member of the sirtuin family that functions as an NAD+-dependent metabolic regulator and is distinguished by its prominent ADP-ribosyltransferase activity toward glutamate dehydrogenase (GDH) [1][2]. Mechanistically, SIRT4 represses mitochondrial glutamine and glutamate metabolism through ADP-ribosylation-mediated inhibition of GDH, thereby reducing amino acid-stimulated insulin secretion and modulating cellular energy homeostasis[1][2][3]. Beyond GDH regulation, SIRT4 participates in broader metabolic control, including the regulation of fatty acid oxidation, branched-chain amino acid catabolism, and mitochondrial metabolic adaptation during nutrient stress[4][5][6]. These functions position SIRT4 as an important regulator of mitochondrial metabolism and endocrine signaling in pancreatic β cells and other metabolically active tissues[1][4]. In disease contexts, reduced SIRT4 activity or expression has been associated with enhanced glutamine utilization and tumor progression, whereas restoration of SIRT4 suppresses proliferation and promotes tumor-suppressive metabolic programs in multiple experimental cancer models[7][8][9]. Compared with related mitochondrial isoforms such as SIRT3 and SIRT5, which primarily function through deacylation-dependent activation or remodeling of metabolic enzymes, SIRT4 is notable for suppressing metabolic flux through ADP-ribosylation-mediated inhibition of key mitochondrial targets[1][10]. For experimental applications, selective SIRT4 inhibitors have recently been reported, providing emerging tools for mechanistic studies of mitochondrial metabolism, insulin secretion, and cancer-associated metabolic reprogramming[11].
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Subcellular Localization
Mitochondrion matrix
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Expression
Tissue_specificity:It can be detected in both vascular smooth muscle and striated muscle. It can also be detected in insulin-secreting β-cells of the Langerhans islet (protein level) . It is widely expressed. Expression is weaker in leukocytes and the fetal thymus.
Induction:Induced by glutamine (at protein level) -
Subunit
Interacts with GLUD1, IDE and SLC25A5 (PubMed:16959573, PubMed:17715127). Interacts with DLAT and PDHX (PubMed:25525879). Interacts with MCCC1 (via the biotin carboxylation domain) (PubMed:23438705). Interacts with PCCA and PC (PubMed:23438705)
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SwissProt ID
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Synonyms
SIR2L4
Documentation
References
[1]. Haigis MC, et al. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. Cell. 2006 Sep 8;126(5):941-54. [Content Brief]
[2]. Caride AJ, et al. The plasma membrane Ca2+ pump isoform 4a differs from isoform 4b in the mechanism of calmodulin binding and activation kinetics: implications for Ca2+ signaling. J Biol Chem. 2007 Aug 31;282(35):25640-8. [Content Brief]
[3]. Zaganjor E, et al. SIRT4 Is a Regulator of Insulin Secretion. Cell Chem Biol. 2017;24(6):656-658.
[5]. Zaganjor E, et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis. Cell Rep. 2021 Jul 13;36(2):109345. [Content Brief]
[6]. Wood JG, et al. Sirt4 is a mitochondrial regulator of metabolism and lifespan in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1564-1569. [Content Brief]
[8]. Cai G, et al. SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism. Sci Rep. 2022 Jul 16;12(1):12208. [Content Brief]
[9]. Wang C, et al. Mammalian SIRT4 is a tumor suppressor of clear cell renal cell carcinoma by inhibiting cancer proliferation, migration and invasion. Cancer Biomark. 2020;29(4):453-462. [Content Brief]
[11]. Pannek M, et al. Specific Inhibitors of Mitochondrial Deacylase Sirtuin 4 Endowed with Cellular Activity. J Med Chem. 2024 Feb 8;67(3):1843-1860. [Content Brief]
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