SIRT4 Antibody (YA4600)
(Synonyms: SIR2L4)
Based on 1 Customer Validation
SIRT4 Antibody (YA4600) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIRT4.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
ICC/IF, FC, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS with 0.05% sodium azide
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
SIRT4 Antibody (YA4600) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIRT4.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 35 kDa
Purified recombinant fragment of human SIRT4 (AA: 215-314) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS with 0.05% sodium azide
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
SIRT4 is a mitochondrial member of the sirtuin family that functions as an NAD+-dependent metabolic regulator and is distinguished by its prominent ADP-ribosyltransferase activity toward glutamate dehydrogenase (GDH) [1][2]. Mechanistically, SIRT4 represses mitochondrial glutamine and glutamate metabolism through ADP-ribosylation-mediated inhibition of GDH, thereby reducing amino acid-stimulated insulin secretion and modulating cellular energy homeostasis[1][2][3]. Beyond GDH regulation, SIRT4 participates in broader metabolic control, including the regulation of fatty acid oxidation, branched-chain amino acid catabolism, and mitochondrial metabolic adaptation during nutrient stress[4][5][6]. These functions position SIRT4 as an important regulator of mitochondrial metabolism and endocrine signaling in pancreatic β cells and other metabolically active tissues[1][4]. In disease contexts, reduced SIRT4 activity or expression has been associated with enhanced glutamine utilization and tumor progression, whereas restoration of SIRT4 suppresses proliferation and promotes tumor-suppressive metabolic programs in multiple experimental cancer models[7][8][9]. Compared with related mitochondrial isoforms such as SIRT3 and SIRT5, which primarily function through deacylation-dependent activation or remodeling of metabolic enzymes, SIRT4 is notable for suppressing metabolic flux through ADP-ribosylation-mediated inhibition of key mitochondrial targets[1][10]. For experimental applications, selective SIRT4 inhibitors have recently been reported, providing emerging tools for mechanistic studies of mitochondrial metabolism, insulin secretion, and cancer-associated metabolic reprogramming[11].
-
Subcellular Localization
Mitochondrion matrix
-
Expression
Tissue_specificity:It can be detected in both vascular smooth muscle and striated muscle. It can also be detected in insulin-secreting β-cells of the Langerhans islet (protein level) . It is widely expressed. Expression is weaker in leukocytes and the fetal thymus.
Induction:Induced by glutamine (at protein level) -
Subunit
Interacts with GLUD1, IDE and SLC25A5 (PubMed:16959573, PubMed:17715127). Interacts with DLAT and PDHX (PubMed:25525879). Interacts with MCCC1 (via the biotin carboxylation domain) (PubMed:23438705). Interacts with PCCA and PC (PubMed:23438705)
-
SwissProt ID
-
Synonyms
SIR2L4
Documentation
-
Data Sheet (263 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
User Guide for Antibodies (1077 KB)
References
[1]. Haigis MC, et al. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. Cell. 2006 Sep 8;126(5):941-54. [Content Brief]
[2]. Caride AJ, et al. The plasma membrane Ca2+ pump isoform 4a differs from isoform 4b in the mechanism of calmodulin binding and activation kinetics: implications for Ca2+ signaling. J Biol Chem. 2007 Aug 31;282(35):25640-8. [Content Brief]
[3]. Zaganjor E, et al. SIRT4 Is a Regulator of Insulin Secretion. Cell Chem Biol. 2017;24(6):656-658.
[5]. Zaganjor E, et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis. Cell Rep. 2021 Jul 13;36(2):109345. [Content Brief]
[6]. Wood JG, et al. Sirt4 is a mitochondrial regulator of metabolism and lifespan in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1564-1569. [Content Brief]
[8]. Cai G, et al. SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism. Sci Rep. 2022 Jul 16;12(1):12208. [Content Brief]
[9]. Wang C, et al. Mammalian SIRT4 is a tumor suppressor of clear cell renal cell carcinoma by inhibiting cancer proliferation, migration and invasion. Cancer Biomark. 2020;29(4):453-462. [Content Brief]
[11]. Pannek M, et al. Specific Inhibitors of Mitochondrial Deacylase Sirtuin 4 Endowed with Cellular Activity. J Med Chem. 2024 Feb 8;67(3):1843-1860. [Content Brief]
Powered by Bioz
