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Art. -Nr.: HY-187166

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Hamiformamide

Hamiformamide is a nitric oxide production inhibitor with an IC₅₀ of 49.1 μM, which is isolated from Hamigera avellanea IFM 52957. Hamiformamide inhibits nitric oxide production in macrophage-like cells. Hamiformamide modulates host immune signals.

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CAS. Nr.: 179419-82-0
Formel: C₁₉H₁₈N₂O₄
Molecular Weight:338.36

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Hamiformamide Chemical Structure

CAS. Nr.: 179419-82-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Protokoll (1)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle NO Synthase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A498	IC₅₀	1.72 μM Compound: 4	Cytotoxicity against human A498 cells Cytotoxicity against human A498 cells	[PMID: 21667925]
ACHN	IC₅₀	1.37 μM Compound: 4	Cytotoxicity against human ACHN cells Cytotoxicity against human ACHN cells	[PMID: 21667925]
HOP-92	IC₅₀	2.5 μM Compound: 4	Cytotoxicity against human HOP92 cells Cytotoxicity against human HOP92 cells	[PMID: 21667925]
Malme-3M	IC₅₀	2.5 μM Compound: 4	Cytotoxicity against human MALME-3M cells Cytotoxicity against human MALME-3M cells	[PMID: 21667925]
MOLT-4	IC₅₀	1.81 μM Compound: 4	Cytotoxicity against human MOLT4 cells Cytotoxicity against human MOLT4 cells	[PMID: 21667925]
NCI-H460	IC₅₀	1.84 μM Compound: 4	Cytotoxicity against human NCI-H460 cells Cytotoxicity against human NCI-H460 cells	[PMID: 21667925]
SF-295	IC₅₀	2.5 μM Compound: 4	Cytotoxicity against human SF295 cells Cytotoxicity against human SF295 cells	[PMID: 21667925]
SK-MEL-28	IC₅₀	2.03 μM Compound: 4	Cytotoxicity against human SK-MEL-28 cells Cytotoxicity against human SK-MEL-28 cells	[PMID: 21667925]
SK-MEL-5	IC₅₀	2.5 μM Compound: 4	Cytotoxicity against human SK-MEL-5 cells Cytotoxicity against human SK-MEL-5 cells	[PMID: 21667925]
SK-OV-3	IC₅₀	2.13 μM Compound: 4	Cytotoxicity against human SKOV3 cells Cytotoxicity against human SKOV3 cells	[PMID: 21667925]
U-251	IC₅₀	2.5 μM Compound: 4	Cytotoxicity against human U251 cells Cytotoxicity against human U251 cells	[PMID: 21667925]
UACC-62	IC₅₀	2.5 μM Compound: 4	Cytotoxicity against human UACC62 cells Cytotoxicity against human UACC62 cells	[PMID: 21667925]

In Vitro

Hamiformamide (5-100 μM; 2 h pre-incubation, 24 h incubation with LPS) potently inhibits LPS-induced nitric oxide production in RAW264 macrophage-like cells with an IC₅₀ of 49.1 µM while maintaining high cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS. Nr. 179419-82-0
Molecular Weight 338.36
Formel C₁₉H₁₈N₂O₄
SMILES

COC1=CC=C(/C=C(NC=O)/C(NC=O)=C/C2=CC=C(O)C=C2)C=C1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Protokoll

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Saito S, et al. Activation of hamiformamide production in the thermotolerant fungus Hamigera avellanea triggered by co-culture with animal immune cells. J Antibiot (Tokyo). 2026 Jul 1. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

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100 mg

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