Bim BH3, Peptide IV TFA
Bim BH3, Peptide IV TFA is an Aβ42-binding peptide and the pro-apoptotic BH3 domain of the BIM protein. Bim BH3, Peptide IV TFA binds Aβ42 with a Kd of 7.1 μM. Bim BH3, Peptide IV TFA modulates Aβ42 structure, fibrillization pathways, aggregate morphology, and membrane interactions, inhibiting fibril formation while enhancing protofibril assembly. Bim BH3, Peptide IV TFA promotes Aβ42 β-sheet formation and accelerates aggregation. Bim BH3, Peptide IV TFA enhances Aβ42 membrane internalization and bilayer stiffening. Bim BH3, Peptide IV TFA induces neuronal cell death by enhancing BH3-induced membrane interactions of Aβ42 prefibrillar species. Bim BH3, Peptide IV TFA can be used for research on Alzheimer's disease.
For research use only. We do not sell to patients.
- Formula: C145H222N44O41S.xC2HF3O2
- Molecular Weight:3269.65 (free acid)
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
Aβ42 7.1 μM (Kd) |
Bim |
In Vitro
Bim BH3, Peptide IV (5-20 μM) TFA binds to Aβ42 monomers with a Kd of 7.1 x 10-6 M[1].
Bim BH3, Peptide IV (14 hrs) TFA enhances Aβ42 aggregation and fibrillation in a concentration-dependent manner[1].
Bim BH3, Peptide IV (14 h) TFA alters the morphology and increases the abundance of Aβ42 aggregates, forming smaller and thicker fibrillar species[1].
Bim BH3, Peptide IV (1:0.5-1:1 mole ratio, 12.5-25 μM; 0-12 h) TFA accelerates the random coil-to-β sheet structural transformation of Aβ42 in a concentration-dependent manner[1].
Bim BH3, Peptide IV (12.5-25 μM; 10 min-48 h) TFA significantly modulates Aβ42 fibril assembly, promoting the formation of abundant pre-fibrillar and protofibril structures while inhibiting the formation of mature fibrils[1].
Bim BH3, Peptide IV (15-45 μM; 48 h) TFA enhances Aβ42-induced toxicity in SH-SY5Y cells in a concentration-dependent manner, reducing cell viability to around 40%[1].
Bim BH3, Peptide IV (15 μM) TFA enhances Aβ42-induced morphological changes associated with cell death in SH-SY5Y cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y neuroblastoma cell line
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Concentration:15 μM; 30 μM; 45 μM
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Incubation Time:48 hours
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Result:Decreased cell viability to around 40% at the highest BIM-BH3 concentration.
Significantly increased the fractions of apoptotic and necrotic cells.
Did not produce toxic effects when the BIM-BH3 peptide was alone.
Chemical Information
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Molecular Weight 3269.65 (free acid)
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Formula C145H222N44O41S.xC2HF3O2
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Sequence
Asp-Met-Arg-Pro-Glu-Ile-Trp-Ile-Ala-Gln-Glu-Leu-Arg-Arg-Ile-Gly-Asp-Glu-Phe-Asn-Ala-Tyr-Tyr-Ala-Arg-Arg
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Sequence Shortening
DMRPEIWIAQELRRIGDEFNAYYARR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bim BH3, Peptide IV TFA Related Classifications
HY-P1889A Related Classifications
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