Bim BH3, Peptide IV
Based on 1 Customer Validation
Bim BH3, Peptide IV is an Aβ42-binding peptide and the pro-apoptotic BH3 domain of the BIM protein. Bim BH3, Peptide IV binds Aβ42 with a Kd of 7.1 μM. Bim BH3, Peptide IV modulates Aβ42 structure, fibrillization pathways, aggregate morphology, and membrane interactions, inhibiting fibril formation while enhancing protofibril assembly. Bim BH3, Peptide IV promotes Aβ42 β-sheet formation and accelerates aggregation. Bim BH3, Peptide IV enhances Aβ42 membrane internalization and bilayer stiffening. Bim BH3, Peptide IV induces neuronal cell death by enhancing BH3-induced membrane interactions of Aβ42 prefibrillar species. Bim BH3, Peptide IV can be used for research on Alzheimer's disease.
For research use only. We do not sell to patients.
- Purity : 99.24%
- CAS No.: 721885-31-0
- Formula: C145H222N44O41S
- Molecular Weight:3269.65
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
Aβ42 7.1 μM (Kd) |
Bim |
In Vitro
Bim BH3, Peptide IV (5-20 μM) binds to Aβ42 monomers with a Kd of 7.1 x 10-6 M[1].
Bim BH3, Peptide IV (14 hrs) enhances Aβ42 aggregation and fibrillation in a concentration-dependent manner[1].
Bim BH3, Peptide IV (14 h) alters the morphology and increases the abundance of Aβ42 aggregates, forming smaller and thicker fibrillar species[1].
Bim BH3, Peptide IV (1:0.5-1:1 mole ratio, 12.5-25 μM; 0-12 h) accelerates the random coil-to-β sheet structural transformation of Aβ42 in a concentration-dependent manner[1].
Bim BH3, Peptide IV (12.5-25 μM; 10 min-48 h) significantly modulates Aβ42 fibril assembly, promoting the formation of abundant pre-fibrillar and protofibril structures while inhibiting the formation of mature fibrils[1].
Bim BH3, Peptide IV (15-45 μM; 48 h) enhances Aβ42-induced toxicity in SH-SY5Y cells in a concentration-dependent manner, reducing cell viability to around 40%[1].
Bim BH3, Peptide IV (15 μM) enhances Aβ42-induced morphological changes associated with cell death in SH-SY5Y cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y neuroblastoma cell line
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Concentration:15 μM; 30 μM; 45 μM
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Incubation Time:48 hours
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Result:Decreased cell viability to around 40% at the highest BIM-BH3 concentration.
Significantly increased the fractions of apoptotic and necrotic cells.
Did not produce toxic effects when the BIM-BH3 peptide was alone.
Chemical Information
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CAS No. 721885-31-0
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Appearance Solid
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Molecular Weight 3269.65
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Formula C145H222N44O41S
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Color White to off-white
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Sequence Shortening
DMRPEIWIAQELRRIGDEFNAYYARR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (30.58 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.3058 mL | 1.5292 mL | 3.0584 mL | 7.6461 mL |
| 5 mM | 0.0612 mL | 0.3058 mL | 0.6117 mL | 1.5292 mL | |
| 10 mM | 0.0306 mL | 0.1529 mL | 0.3058 mL | 0.7646 mL | |
| 15 mM | 0.0204 mL | 0.1019 mL | 0.2039 mL | 0.5097 mL | |
| 20 mM | 0.0153 mL | 0.0765 mL | 0.1529 mL | 0.3823 mL | |
| 25 mM | 0.0122 mL | 0.0612 mL | 0.1223 mL | 0.3058 mL | |
| 30 mM | 0.0102 mL | 0.0510 mL | 0.1019 mL | 0.2549 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Bim BH3, Peptide IV Related Classifications
HY-P1889 Related Classifications
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