AChE-IN-109
AChE-IN-109 is a potent mixed-type cholinesterase inhibitor with significantly stronger inhibitory activity against AChE than BChE. AChE-IN-109 has IC50 values of 0.55 μM and 12.45 μM against AChE and BChE, respectively. AChE-IN-109 inhibits cholinesterases through a mixed-type mechanism, binds to both the catalytic active site (CAS) and peripheral anionic site (PAS) of AChE. AChE-IN-109 can be used for the study of Alzheimer’s disease (AD).
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- Formule: C28H31N7O2
- Masse moléculaire:497.59
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
AChE 0.55 μM (IC50) |
BChE 12.45 μM (IC50) |
In Vitro
AChE-IN-109 (11n) (10 min pre-incubation; 10 min reaction) potently inhibits electric eel acetylcholinesterase with an IC50 of 0.55 μM and horse serum butyrylcholinesterase with an IC50 of 12.45 μM, exhibiting 22.43-fold selectivity over horse serum butyrylcholinesterase[1].
AChE-IN-109 functions as a mixed-type inhibitor that binds to both the catalytic active site (CAS) and peripheral anionic site (PAS) of AChE[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Masse moléculaire 497.59
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Formule C28H31N7O2
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SMILES
O=[N+](C1=CC=C(C2=NC(NCCCN3CCN(CC4=CC=CC=C4)CC3)=NN2C5=CC=CC=C5)C=C1)[O-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Carbohydrates and Mucins: Periodic Acid-Schiff (PAS) Staining
Periodic acid-Schiff staining detects tissue carbohydrates and mucosubstances by oxidizing carbohydrate glycol groups with periodic acid to generate aldehydes, which then react with Schiff reagent to produce a magenta reaction product; classic reports established the method for mucin and polysaccharide-containing structures in fixed tissue sections. PAS staining can demonstrate neutral mucins and goblet-cell mucin, but it is not specific for mucin because glycogen and other PAS-positive tissue components can also stain; diastase/PAS-D is used when glycogen removal is needed to distinguish glycogen-dependent PAS signal from non-glycogen PAS-positive mucosubstances.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AChE-IN-109 Related Classifications
HY-181851 Related Classifications
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