Calcium 2-hydroxypropanoate pentahydrate
Based on 19 publication(s) in Google Scholar
Calcium 2-hydroxypropanoate (pentahydrate) is a hydroxycarboxylic acid receptor 1 (HCAR1) activator and an epigenetic modulator inducing lysine residues lactylation. Calcium 2-hydroxypropanoate (pentahydrate) is a glycolysis end-product, bridging the gap between glycolysis and oxidative phosphorylation. Calcium 2-hydroxypropanoate (pentahydrate) is an oncometabolite and has immune protective role of lactate in anti-tumor immunity.
For research use only. We do not sell to patients.
- Purity: 99.8%
- CAS No.: 5743-47-5
- Formula: C3H6O3.0.5Ca.2.5H2O
- Molecular Weight:155.17
-
Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Immunology/Inflammation Compound Library
HY-L007
-
Metabolism/Protease Compound Library
HY-L012
-
Anti-Cancer Compound Library
HY-L025
-
Glycolysis Compound Library
HY-L058
-
Anti-Cancer Metabolism Compound Library
HY-L083
-
Glucose Metabolism Compound Library
HY-L092
-
High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) Calcium 2-hydroxypropanoate pentahydrate
More- Cell Res. 2023 Sep;33(9):679-698. [Abstract]
- Nat Commun. 2025 Dec 19. [Abstract]
- J Exp Clin Cancer Res. 2024 Mar 5;43(1):68. [Abstract]
- Adv Sci (Weinh). 2025 Jun;12(21):e2415530. [Abstract]
- Adv Sci (Weinh). 2025 May;12(20):e2413709. [Abstract]
- Adv Sci (Weinh). 2024 Dec;11(47):e2406184. [Abstract]
- Environ Sci Technol. 2025 Aug 5;59(30):15705-15719. [Abstract]
- Cell Death Dis. 2025 Mar 20;16(1):193. [Abstract]
- Cancer Lett. 2026 Sep 28:656:218655. [Abstract]
- Phytomedicine. 2025 May:140:156628. [Abstract]
- J Anim Sci Biotechnol. 2024 Jun 11;15(1):86. [Abstract]
- Stem Cell Res Ther. 2025 Oct 1;16(1):538. [Abstract]
- Cell Rep. 2026 Apr 27;45(5):117317. [Abstract]
- Cell Rep. 2025 Apr 9;44(4):115545. [Abstract]
- Int Immunopharmacol. 2025 Apr 4:151:114269. [Abstract]
- Biol Direct. 2025 May 26;20(1):64. [Abstract]
- Neurotox Res. 2026 Apr 24;44(3):24. [Abstract]
- 3 Biotech. 2026 May;16(5):181. [Abstract]
- Res Sq. 2026 Feb 19.
Customer Validation & Images
-
WB
-
IF
-
WB
-
IP
All Endogenous Metabolite Isoforms
More
Biological Activity
Chemical Information
-
CAS No. 5743-47-5
-
Appearance Solid
-
Molecular Weight 155.17
-
Formula C3H6O3.0.5Ca.2.5H2O
-
Color White to light yellow
-
SMILES
O=C(C(C)O)O.[Ca].O.[0.5].[2.5]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (19)
-
Journal Impact Factor
-
Most Recent
-
Cell Res
α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure. [Abstract]2023 Sep;33(9):679-698. PMID: 37443257
Calcium 2-hydroxypropanoate pentahydrate purchased from MedChemExpress. Usage Cited in: Cell Res. 2023 Sep;33(9):679-698. [Abstract]
Lactate (NALA) (300 mM; 24 h) significantly upregulates α-MHC K1897 lactylation in H9c2 cells.
-
Nat Commun
IDH2 lactylation promotes angiogenesis in murine diabetic myocardial infarction via blocking Cav1-eNOS interaction. [Abstract]2025 Dec 19. PMID: 41419771 -
J Exp Clin Cancer Res
MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis. [Abstract]2024 Mar 5;43(1):68. PMID: 38439082
Calcium 2-hydroxypropanoate pentahydrate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Mar 5;43(1):68. [Abstract]
Lactate sodium increases IGF-1R levels in PI-resistant KAS-6/1 and U266 cells.
-
Adv Sci (Weinh)
High Sugar Induced RCC2 Lactylation Drives Breast Cancer Tumorigenicity Through Upregulating MAD2L1. [Abstract]2025 Jun;12(21):e2415530. PMID: 40145796 -
Adv Sci (Weinh)
UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling. [Abstract]2025 May;12(20):e2413709. PMID: 40150862
Calcium 2-hydroxypropanoate pentahydrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(20):e2413709. [Abstract]
Western blot of UGDH lactylation in chondrocytes with Lactic acid sodium (lactate) (25 mM) treatment for 24 h.
-
Adv Sci (Weinh)
PKM2-Driven Lactate Overproduction Triggers Endothelial-To-Mesenchymal Transition in Ischemic Flap via Mediating TWIST1 Lactylation. [Abstract]2024 Dec;11(47):e2406184. PMID: 39474980 -
Environ Sci Technol
Chronic Dietary Exposure to Environmental Levels of Glyphosate Increases the Risk of Reproductive Dysfunction in Male Mice. [Abstract]2025 Aug 5;59(30):15705-15719. PMID: 40698942 -
Cell Death Dis
Histone lactylation enhances GCLC expression and thus promotes chemoresistance of colorectal cancer stem cells through inhibiting ferroptosis. [Abstract]2025 Mar 20;16(1):193. PMID: 40113760
Calcium 2-hydroxypropanoate pentahydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Mar 20;16(1):193. [Abstract]
Lactate sodium (5-15 mM; 24 h) results in increased H4K12la (red) levels in CCSCs compared to the control group.
-
Cancer Lett
Targeting C/EBPβ/SCD1/C16:1 loop-driven macrophage M2 polarization by Bruceine D improves anti-PD-1 immunotherapy in colorectal cancer. [Abstract]2026 Sep 28:656:218655. PMID: 42250753 -
Phytomedicine
Effective-compounds of Jinshui Huanxian Formula acts as an SRC inhibitor to inhibit HK2-mediated H3K18 lactation and improve pulmonary fibrosis. [Abstract]2025 May:140:156628. PMID: 40090047 -
J Anim Sci Biotechnol
The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation. [Abstract]2024 Jun 11;15(1):86. PMID: 38858724 -
Stem Cell Res Ther
H3K18 lactylation-hexokinase 2 positive feedback loop promotes osteogenesis of ASPCs in facial infiltrating lipomatosis. [Abstract]2025 Oct 1;16(1):538. PMID: 41035068 -
Cell Rep
Heat shock protein 90β lactylation acts as a metabolic switch to drive cerebrovascular apoptosis under heat stress. [Abstract]2026 Apr 27;45(5):117317. PMID: 42048184 -
Cell Rep
High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy. [Abstract]2025 Apr 9;44(4):115545. PMID: 40215164 -
Int Immunopharmacol
Histone lactylation protects against sevoflurane-induced cognitive impairment by regulating YTHDF3/PRDX3 mediated microglial pyroptosis in neonatal mice. [Abstract]2025 Apr 4:151:114269. PMID: 40022822 -
Biol Direct
LDHA-mediated YAP lactylation promotes the tumor progression of hepatocellular carcinoma by inducing YAP dephosphorylation and activation. [Abstract]2025 May 26;20(1):64. PMID: 40414964 -
Neurotox Res
TDP-43 Dysfunction Causes Hyper-Lactate State, Increased AARS1 Expression and Enhanced Protein Lactylation. [Abstract]2026 Apr 24;44(3):24. PMID: 42029805 -
3 Biotech
Touxie Quyin Compound regulates histone lactylation-mediated Fabp4/5 and tissue-resident memory T cells to improve psoriasis recurrence in mice. [Abstract]2026 May;16(5):181. PMID: 42077602 -
Solvent & Solubility
DMSO : 100 mg/mL (644.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (32.22 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (32.22 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Lucia Longhitano, et al. Lactate Rewrites the Metabolic Reprogramming of Uveal Melanoma Cells and Induces Quiescence Phenotype. Int J Mol Sci. 2022 Dec 20;24(1):24. [Content Brief]
[2]. Qiang Feng, et al. Lactate increases stemness of CD8 + T cells to augment anti-tumor immunity. Nat Commun. 2022 Sep 6;13(1):4981. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.4445 mL | 32.2227 mL | 64.4454 mL | 161.1136 mL |
| 5 mM | 1.2889 mL | 6.4445 mL | 12.8891 mL | 32.2227 mL | |
| 10 mM | 0.6445 mL | 3.2223 mL | 6.4445 mL | 16.1114 mL | |
| 15 mM | 0.4296 mL | 2.1482 mL | 4.2964 mL | 10.7409 mL | |
| 20 mM | 0.3222 mL | 1.6111 mL | 3.2223 mL | 8.0557 mL | |
| 25 mM | 0.2578 mL | 1.2889 mL | 2.5778 mL | 6.4445 mL | |
| 30 mM | 0.2148 mL | 1.0741 mL | 2.1482 mL | 5.3705 mL | |
| 40 mM | 0.1611 mL | 0.8056 mL | 1.6111 mL | 4.0278 mL | |
| 50 mM | 0.1289 mL | 0.6445 mL | 1.2889 mL | 3.2223 mL | |
| 60 mM | 0.1074 mL | 0.5370 mL | 1.0741 mL | 2.6852 mL | |
| 80 mM | 0.0806 mL | 0.4028 mL | 0.8056 mL | 2.0139 mL | |
| 100 mM | 0.0644 mL | 0.3222 mL | 0.6445 mL | 1.6111 mL |
Calcium 2-hydroxypropanoate pentahydrate Related Classifications
HY-B2227D Related Classifications
Powered by Bioz