Capsaicin (Purity 65%)

Name: Capsaicin (Purity 65%)
Brand: MedChemExpress
SKU: HY-10448A
Rating: 5.0 (21 reviews)

Cat. No.: HY-10448A Purity: 99.20%: Data Sheet
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Capsaicin (Purity 65%) is a mixture of Capsaicin and Dihydrocapsaicin (Ratio >2:1). Capsaicin (Purity 65%) is an orally active capsaicin receptor (TRPV1) agonist.

For research use only. We do not sell to patients.

Capsaicin (Purity 65%) Chemical Structure

CAS No. : 404-86-4

Size	Stock	Quantity

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of Capsaicin (Purity 65%):

Capsaicin In-stock
Capsaicin-d₃ Get quote
Capsaicin-d₇ Get quote
Capsaicin (Purity 65%) (Standard) Get quote

21 Publications Citing Use of MCE Capsaicin (Purity 65%)

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•Related Small Molecules:

•Related Proteins:

View All TRP Channel Isoform Specific Products:

View All Isoforms

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

View All Endogenous Metabolite Isoform Specific Products:

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

Capsaicin (Purity 65%) is a mixture of Capsaicin and Dihydrocapsaicin (Ratio >2:1). Capsaicin (Purity 65%) is an orally active capsaicin receptor (TRPV1) agonist^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A2058	IC₅₀	>100 μM Compound: Capsaicin	Cytotoxicity against human A2058 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human A2058 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay	[PMID: 31104785]
B16-F10	IC₅₀	>100 μM Compound: Capsaicin	Cytotoxicity against mouse B16F10 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against mouse B16F10 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay	[PMID: 31104785]
B16-F10	IC₅₀	117 μM Compound: 1	Antiproliferative activity against B16-F10 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Antiproliferative activity against B16-F10 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 37000154]
BGC-823	IC₅₀	4.659 μM Compound: 1	Antiproliferative activity against human BGC-823 cells assessed as reduction in cell viability incubated for 5 days by MTS assay Antiproliferative activity against human BGC-823 cells assessed as reduction in cell viability incubated for 5 days by MTS assay	[PMID: 33581557]
CHO	EC₅₀	25.9 nM Compound: 1; CAP	Agonist activity at human TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method Agonist activity at human TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method	[PMID: 31702924]
CHO	EC₅₀	44.8 nM Compound: 1; CAP	Agonist activity at rat TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method Agonist activity at rat TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method	[PMID: 31702924]
CHO	EC₅₀	44.8 nM Compound: Capsaicin	In vitro [Ca2+] influx relative to capsaicin by Rat Vanilloid receptor (VR1) expressing CHO cells In vitro [Ca2+] influx relative to capsaicin by Rat Vanilloid receptor (VR1) expressing CHO cells	[PMID: 12825950]
CHO	EC₅₀	44.8 nM Compound: Capsaicin	Agonist activity at rat TRPV1 receptor expressed in CHO cells assessed as calcium uptake Agonist activity at rat TRPV1 receptor expressed in CHO cells assessed as calcium uptake	[PMID: 19135377]
HBMEC-2	EC₅₀	16.37 μM Compound: Capsaicin	Neuroprotective activity against H2O2-induced cell damage in human HBMEC2 cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay Neuroprotective activity against H2O2-induced cell damage in human HBMEC2 cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay	[PMID: 31541868]
HCT-116	IC₅₀	40.16 μM Compound: Capsaicin	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay	[PMID: 34795858]
HEK293	EC₅₀	0.0039 μM Compound: Capsaicin	Agonist activity at human TRPV1 ion channel expressed in HEK293 cells assessed as calcium influx by fluo-4-Am-based fluorimetry Agonist activity at human TRPV1 ion channel expressed in HEK293 cells assessed as calcium influx by fluo-4-Am-based fluorimetry	[PMID: 22257892]
HEK293	EC₅₀	0.0053 μM Compound: 1a	Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as Ca2+ influx by Fluo-4 dye based assay Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as Ca2+ influx by Fluo-4 dye based assay	[PMID: 25666822]
HEK293	EC₅₀	0.02 μM Compound: capsaicin	Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level	[PMID: 19361197]
HEK293	EC₅₀	40 nM Compound: 1a	Agonist activity at human TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level Agonist activity at human TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level	[PMID: 19951840]
HEK293	EC₅₀	40 nM Compound: capsaicin	Agonist activity at human recombinant TRPV1 expressed in human HEK293 cells Agonist activity at human recombinant TRPV1 expressed in human HEK293 cells	[PMID: 17046253]
HEK293	IC₅₀	0.008 μM Compound: 1a	Antagonist activity against human recombinant TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced Ca2+ influx pre-treated 5 mins before capsaicin addition by Fluo-4 dye based assay Antagonist activity against human recombinant TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced Ca2+ influx pre-treated 5 mins before capsaicin addition by Fluo-4 dye based assay	[PMID: 25666822]
HeLa	IC₅₀	>50 μM Compound: CAP	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 29685681]
Jurkat	IC₅₀	~ 128 μM Compound: 1	Induction of apoptosis in human Jurkat cells assessed as increase in apoptotic cells by propidium iodide staining based flow cytometry Induction of apoptosis in human Jurkat cells assessed as increase in apoptotic cells by propidium iodide staining based flow cytometry	[PMID: 37000154]
K562	IC₅₀	49.41 μM Compound: CAP	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 29685681]
MCF7	IC₅₀	~ 53 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 37000154]
MCF7	IC₅₀	100 μM Compound: Capsaicin	Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition by MTT assay	[PMID: 33508189]
MCF7	IC₅₀	49.97 μM Compound: CAP	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 29685681]
MDA-MB-231	IC₅₀	120 μM Compound: 24	Antiproliferative activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay	[PMID: 32686940]
NCI-H1299	IC₅₀	>=200 μM Compound: 1	Antiproliferative activity against human NCI-H1299 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Antiproliferative activity against human NCI-H1299 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 37000154]
NCI-H460	IC₅₀	30.66 μM Compound: Capsaicin	Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay	[PMID: 34795858]
SH-SY5Y	EC₅₀	14.02 μM Compound: Capsaicin	Neuroprotective activity against H2O2-induced cell damage in human SH-SY5Y cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay Neuroprotective activity against H2O2-induced cell damage in human SH-SY5Y cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay	[PMID: 31541868]
SH-SY5Y	EC₅₀	2.32 μM Compound: Capsaicin	Agonist activity at rat TRPV1 expressed in human SH-SY5Y cells assessed as intracellular calcium accumulation by Fluo-4 NW dye based fluorescence assay Agonist activity at rat TRPV1 expressed in human SH-SY5Y cells assessed as intracellular calcium accumulation by Fluo-4 NW dye based fluorescence assay	[PMID: 30783490]
SK-MEL-25	IC₅₀	>100 μM Compound: Capsaicin	Cytotoxicity against human SK-MEL-25 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human SK-MEL-25 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay	[PMID: 31104785]
SK-OV-3	IC₅₀	22.03 μM Compound: Capsaicin	Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay	[PMID: 34795858]
T47D	IC₅₀	100 μM Compound: Capsaicin	Antiproliferative activity in human T47D cells assessed as cell growth inhibition by MTT assay Antiproliferative activity in human T47D cells assessed as cell growth inhibition by MTT assay	[PMID: 33508189]
U-87MG ATCC	IC₅₀	>100 μM Compound: Capsaicin	Cytotoxicity against human U87 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human U87 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay	[PMID: 31104785]

In Vitro

Capsaicin is the main Capsaicinoid in chili peppers, followed by Dihydrocapsaicin. These two compounds provide about twice hotness to the taste and nerves as the minor capsaicinoids^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Capsaicin (Purity 65%) (1-10 mg/kg, oral gavage) increases chlorzoxazone 6-hydroxylase activity and the expression of CYP2E1 in liver microsomes of rats^[4].
Capsaicin (Purity 65%) (10 mg/kg, s.c.) shows the C_max of 104.9 ng/mL for Capsaicin and 54.3 ng/mL for Dihydrocapsaicin, the T_max of 5 h for Capsaicin and 4 h for Dihydrocapsaicin^[5].
Capsaicin is more pungent than Dihydrocapsaicin (HY-N0361)^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

305.41

Formula

C₁₈H₂₇NO₃

CAS No.

404-86-4

Appearance

Solid

Color

White to light yellow

SMILES

CC(C)/C=C/CCCCC(NCC1=CC=C(O)C(OC)=C1)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (327.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2743 mL	16.3714 mL	32.7429 mL
5 mM	0.6549 mL	3.2743 mL	6.5486 mL
10 mM	0.3274 mL	1.6371 mL	3.2743 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.20%

Select Batch:

Data Sheet (273 KB) SDS (806 KB)

COA (244 KB) HNMR (264 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Hoyoun Cho, et al. Development of a database of capsaicinoid contents in foods commonly consumed in Korea. Food Sci Nutr. 2020 Jul 16;8(8):4611-4624. [Content Brief]

[2]. Krishnapura Srinivasan. Biological Activities of Red Pepper (Capsicum annuum) and Its Pungent Principle Capsaicin: A Review. Crit Rev Food Sci Nutr. 2016 Jul 3;56(9):1488-500. [Content Brief]

[3]. Zhang QH, et al. Effects of capsaicin and dihydrocapsaicin on human and rat liver microsomal CYP450 enzyme activities in vitro and in vivo. J Asian Nat Prod Res. 2012;14(4):382-95. [Content Brief]

[4]. Zhang Q, et al. Simultaneous quantification of capsaicin and dihydrocapsaicin in rat plasma using HPLC coupled with tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Aug 15;878(24):2292-7. [Content Brief]

[5]. Joshi SK, et al Comparison of antinociceptive actions of standard analgesics in attenuating capsaicin and nerve-injury-induced mechanical hypersensitivity. Neuroscience. 2006 Dec 1;143(2):587-96. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2743 mL	16.3714 mL	32.7429 mL	81.8572 mL
	5 mM	0.6549 mL	3.2743 mL	6.5486 mL	16.3714 mL
	10 mM	0.3274 mL	1.6371 mL	3.2743 mL	8.1857 mL
	15 mM	0.2183 mL	1.0914 mL	2.1829 mL	5.4571 mL
	20 mM	0.1637 mL	0.8186 mL	1.6371 mL	4.0929 mL
	25 mM	0.1310 mL	0.6549 mL	1.3097 mL	3.2743 mL
	30 mM	0.1091 mL	0.5457 mL	1.0914 mL	2.7286 mL
	40 mM	0.0819 mL	0.4093 mL	0.8186 mL	2.0464 mL
	50 mM	0.0655 mL	0.3274 mL	0.6549 mL	1.6371 mL
	60 mM	0.0546 mL	0.2729 mL	0.5457 mL	1.3643 mL
	80 mM	0.0409 mL	0.2046 mL	0.4093 mL	1.0232 mL
	100 mM	0.0327 mL	0.1637 mL	0.3274 mL	0.8186 mL