Dihydrocapsaicin

Name: Dihydrocapsaicin
Brand: MedChemExpress
SKU: HY-N0361
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0361 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Dihydrocapsaicin, a capsaicin, is a potent and selective TRPV1 (transient receptor potential vanilloid channel 1) agonist. Dihydrocapsaicin reduces AIF, Bax, and Caspase-3 expressions, and increased Bcl-2, Bcl-xL and p-Akt levels. Dihydrocapsaicin enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat.

For research use only. We do not sell to patients.

Dihydrocapsaicin Chemical Structure

CAS No. : 19408-84-5

Size	Price	Stock	Quantity
5 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Dihydrocapsaicin:

Dihydrocapsaicin-d₃ In-stock

1 Publications Citing Use of MCE Dihydrocapsaicin

•Chem Eng J. 2023 Apr 15.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TRP Channel Isoform Specific Products:

View All Isoforms

View All Caspase Isoform Specific Products:

View All Isoforms

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms

View All Akt Isoform Specific Products:

View All Isoforms

View All PI3K Isoform Specific Products:

View All Isoforms

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

TRPV1

PI3K

Caspase 3

Bax

Bcl-2

Bcl-xL

In Vitro

Dihydrocapsaicin (0-100 μM) inhibits platelet aggregation and the activity of clotting factors VIII:C (6.26-100 μM) and IX (25-100 μM)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Dihydrocapsaicin (0.5 mg/kg, IP, once) exhibits hypothermic effect and neuroprotection in rat MCAO models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley rats (adult, male, 300-340 g, subjected to right middle cerebral artery occlusion (MCAO)^[2]
Dosage:	0.5 mg/kg
Administration:	IP, once
Result:	Exhibits hypothermic effect, rectal temperature dropped to approximately 35.0 ℃ at 30 min, stayed at equal or below 35.0 ℃ for approximately 20 min, and then gradually returned to approximately 36.5 ℃ at 120 min. Significantly reduced Ischemia-reperfusion induced infarct volume (36.2% ± 2.5%). Reduces ROS levels at 24 h, and reduced ischemia-reperfusion induced a high level of cell death.

Molecular Weight

307.43

Formula

C₁₈H₂₉NO₃

CAS No.

19408-84-5

Appearance

Solid

Color

White to off-white

SMILES

CC(C)CCCCCCC(NCC1=CC=C(O)C(OC)=C1)=O

Structure Classification

Alkaloids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (325.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2528 mL	16.2639 mL	32.5277 mL
5 mM	0.6506 mL	3.2528 mL	6.5055 mL
10 mM	0.3253 mL	1.6264 mL	3.2528 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (272 KB) SDS (420 KB)

COA (242 KB) HNMR (251 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Gao F, et al. Impairment in function and expression of transient receptor potential vanilloid type 4 in Dahl salt-sensitive rats: significance and mechanism. Hypertension. 2010 Apr;55(4):1018-25. [Content Brief]

[2]. Dihydrocapsaicin, et al. Dihydrocapsaicin (DHC) enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat. Brain Res. 2017 Sep 15;1671:18-25. [Content Brief]

[3]. Adams MJ, et al. Effect of capsaicin and dihydrocapsaicin on in vitro blood coagulation and platelet aggregation. Thromb Res. 2009 Dec;124(6):721-3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2528 mL	16.2639 mL	32.5277 mL	81.3193 mL
	5 mM	0.6506 mL	3.2528 mL	6.5055 mL	16.2639 mL
	10 mM	0.3253 mL	1.6264 mL	3.2528 mL	8.1319 mL
	15 mM	0.2169 mL	1.0843 mL	2.1685 mL	5.4213 mL
	20 mM	0.1626 mL	0.8132 mL	1.6264 mL	4.0660 mL
	25 mM	0.1301 mL	0.6506 mL	1.3011 mL	3.2528 mL
	30 mM	0.1084 mL	0.5421 mL	1.0843 mL	2.7106 mL
	40 mM	0.0813 mL	0.4066 mL	0.8132 mL	2.0330 mL
	50 mM	0.0651 mL	0.3253 mL	0.6506 mL	1.6264 mL
	60 mM	0.0542 mL	0.2711 mL	0.5421 mL	1.3553 mL
	80 mM	0.0407 mL	0.2033 mL	0.4066 mL	1.0165 mL
	100 mM	0.0325 mL	0.1626 mL	0.3253 mL	0.8132 mL