Cat. No.: HY-N0361

Dihydrocapsaicin

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Dihydrocapsaicin, a capsaicin, is a potent and selective TRPV1 (transient receptor potential vanilloid channel 1) agonist. Dihydrocapsaicin reduces AIF, Bax, and Caspase-3 expressions, and increased Bcl-2, Bcl-xL and p-Akt levels. Dihydrocapsaicin enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 19408-84-5
Formula: C₁₈H₂₉NO₃
Molecular Weight:307.43

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Dihydrocapsaicin Chemical Structure

CAS No.: 19408-84-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

TRPV1

PI3K

Caspase 3

Bax

Bcl-2

Bcl-xL

All Bcl-2 Family Isoforms :

All Akt Isoforms :

All TRP Channel Isoforms :

All Caspase Isoforms :

All PI3K Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Astrocyte	EC₅₀	41.83 μM Compound: 15	Antiproliferative activity against mouse astrocyte cells by MTT assay Antiproliferative activity against mouse astrocyte cells by MTT assay	[PMID: 17417631]
HeLa	IC₅₀	>50 μM Compound: Dihydrocapsaicin	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 29685681]
K562	IC₅₀	>50 μM Compound: Dihydrocapsaicin	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 29685681]
MCF7	IC₅₀	>50 μM Compound: Dihydrocapsaicin	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 29685681]
Medulloblastoma cell	EC₅₀	0.02 μM Compound: 15	Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay	[PMID: 17417631]

In Vitro

Dihydrocapsaicin (0-100 μM) inhibits platelet aggregation and the activity of clotting factors VIII:C (6.26-100 μM) and IX (25-100 μM)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Dihydrocapsaicin (0.5 mg/kg, IP, once) exhibits hypothermic effect and neuroprotection in rat MCAO models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley rats (adult, male, 300-340 g, subjected to right middle cerebral artery occlusion (MCAO)^[2]
Dosage:

0.5 mg/kg
Administration:

IP, once
Result:

Exhibits hypothermic effect, rectal temperature dropped to approximately 35.0 ℃ at 30 min, stayed at equal or below 35.0 ℃ for approximately 20 min, and then gradually returned to approximately 36.5 ℃ at 120 min. Significantly reduced Ischemia-reperfusion induced infarct volume (36.2% ± 2.5%). Reduces ROS levels at 24 h, and reduced ischemia-reperfusion induced a high level of cell death.

Chemical Information

CAS No. 19408-84-5
Appearance Solid
Molecular Weight 307.43
Formula C₁₈H₂₉NO₃
Color White to off-white
SMILES

CC(C)CCCCCCC(NCC1=CC=C(O)C(OC)=C1)=O
Structure Classification
- Alkaloids
- Other Alkaloids
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (4)

All Cell Proliferation/Viability AssayIFApoptosis AnalysisHistological Imaging/StainingWB

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Journal Impact Factor

Chem Eng J

2023 Apr 15.
Phytother Res

Dihydrocapsaicin suppresses the STING-mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia-reperfusion injury of perforator skin flap. [Abstract]

2024 May;38(5):2539-2559. PMID: 38459660

Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 May;38(5):2539-2559. [Abstract]

Immunofluorescence staining for CASP3 (red) and CD31 (green) in the skin tissue of control and Dihydrocapsaicin (DHC) (5 mg/kg, p.o) groups.

Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 May;38(5):2539-2559. [Abstract]

Typical HE staining of skin tissues in control and Dihydrocapsaicin (DHC) (5 mg/kg,p.o.) groups.
Cancer Manag Res

Dihydrocapsaicin Secreted by RYK Silenced Bone Marrow-Derived Mesenchymal Stem Cells Triggers Apoptosis of Gastric Cancer Cells. [Abstract]

2026 Mar 21:18:579598. PMID: 41890221

Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598. [Abstract]

The proliferation of NCI-N87 cells was detected by CCK-8 at 12, 24, and 48 h treated with Dihydrocapsaicin (DHC) (20, 40, 60, 80 μM).

Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598. [Abstract]

Effect of Dihydrocapsaicin (DHC) (60, 80 μM) on apoptosis of NCI-N87 cells. The apoptosis of NCI-N87 cells was detected by flow cytometry.

Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598. [Abstract]

The apoptosis-related protein expressions of Bax, Caspase3, and Caspase9 in NCI-N87 cells were detected by WB treated with Dihydrocapsaicin (DHC) (60, 80 μM).
Res Sq

2025 Oct 6.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (325.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2528 mL

16.2639 mL

32.5277 mL

5 mM

0.6506 mL

3.2528 mL

6.5055 mL

10 mM

0.3253 mL

1.6264 mL

3.2528 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Gao F, et al. Impairment in function and expression of transient receptor potential vanilloid type 4 in Dahl salt-sensitive rats: significance and mechanism. Hypertension. 2010 Apr;55(4):1018-25. [Content Brief]

[2]. Dihydrocapsaicin, et al. Dihydrocapsaicin (DHC) enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat. Brain Res. 2017 Sep 15;1671:18-25. [Content Brief]

[3]. Adams MJ, et al. Effect of capsaicin and dihydrocapsaicin on in vitro blood coagulation and platelet aggregation. Thromb Res. 2009 Dec;124(6):721-3. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2528 mL	16.2639 mL	32.5277 mL	81.3193 mL
	5 mM	0.6506 mL	3.2528 mL	6.5055 mL	16.2639 mL
	10 mM	0.3253 mL	1.6264 mL	3.2528 mL	8.1319 mL
	15 mM	0.2169 mL	1.0843 mL	2.1685 mL	5.4213 mL
	20 mM	0.1626 mL	0.8132 mL	1.6264 mL	4.0660 mL
	25 mM	0.1301 mL	0.6506 mL	1.3011 mL	3.2528 mL
	30 mM	0.1084 mL	0.5421 mL	1.0843 mL	2.7106 mL
	40 mM	0.0813 mL	0.4066 mL	0.8132 mL	2.0330 mL
	50 mM	0.0651 mL	0.3253 mL	0.6506 mL	1.6264 mL
	60 mM	0.0542 mL	0.2711 mL	0.5421 mL	1.3553 mL
	80 mM	0.0407 mL	0.2033 mL	0.4066 mL	1.0165 mL
	100 mM	0.0325 mL	0.1626 mL	0.3253 mL	0.8132 mL

Keywords

Dihydrocapsaicin

4 Cited Publications

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Dihydrocapsaicin

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Publications Citing Use of MedChemExpress (MCE) Dihydrocapsaicin

All Bcl-2 Family Isoforms

All Akt Isoforms

All TRP Channel Isoforms

All Caspase Isoforms

All PI3K Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation