Cecropin P1, porcine
Based on 1 Customer Validation
Cecropin P1, porcine is an antibacterial peptide that can be isolated from the upper part of the small intestine of the pig. Cecropin P1, porcine shows antibacterial activity against Gram-negative bacteria. Cecropin P1, porcine shows antiviral activity and inhibits PRRSV infection.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 125667-96-1
- Formula: C147H253N45O43
- Molecular Weight:3338.86
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Cecropin P1, porcine (0-480 μg/mL, 36-96 h) markedly inhibits CH-1a infection and replication in Marc-145 cells[2].
Cecropin P1, porcine (0-480 μg/mL, 36 h) not only displays extracellular virucidal activity against PRRSV (porcine reproductive and respiratory syndrome virus), but also exerts a potent inhibitory effect when added either before, simultaneously with, or after viral inoculation[2].
Cecropin P1, porcine (480 μg/mL, 0-72 h) blocks CH-1a-induced apoptosis during the late phase of infection[2].
Cecropin P1, porcine (0-480 μg/mL, 0-4 h) inhibits viral particle release[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Marc-145 cells
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Concentration:160, 320, and 480 μg/mL
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Incubation Time:36, 48, 72, 96 h
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Result:Significantly inhibited viral infection in a dose-dependent manner at 36 h postinfection. Inhibited CH-1a infection in Marc-145 cells with a 50% effective concentration (EC50) of 112 μg/mL. The 50% cytotoxic concentration (CC50) of Cecropin P1 for Marc-145 cells was estimated to be 719 μg/mL.
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Cell Line:Marc-145 cells
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Concentration:160, 320, and 480 μg/mL
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Incubation Time:36 h
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Result:Significantly inhibited viral infection in a dose-dependent manner at 36 h postinfection. Significantly reduced the expression of the viral N protein when administered with either the pre-, co-, or posttreatment method.
Chemical Information
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CAS No. 125667-96-1
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Appearance Solid
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Molecular Weight 3338.86
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Formula C147H253N45O43
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Color White to off-white
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Sequence
Ser-Trp-Leu-Ser-Lys-Thr-Ala-Lys-Lys-Leu-Glu-Asn-Ser-Ala-Lys-Lys-Arg-Ile-Ser-Glu-Gly-Ile-Ala-Ile-Ala-Ile-Gln-Gly-Gly-Pro-Arg
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Sequence Shortening
SWLSKTAKKLENSAKKRISEGIAIAIQGGPR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
H2O : 50 mg/mL (14.98 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Andersson M, et al. Ascaris nematodes from pig and human make three antibacterial peptides: isolation of cecropin P1 and two ASABF peptides. Cell Mol Life Sci. 2003 Mar;60(3):599-606. [Content Brief]
[2]. Guo C, et al. Cecropin P1 inhibits porcine reproductive and respiratory syndrome virus by blocking attachment. BMC Microbiol. 2014 Nov 18;14:273. [Content Brief]
[3]. Giacometti A, et al. Effect of mono-dose intraperitoneal cecropins in experimental septic shock. Crit Care Med. 2001 Sep;29(9):1666-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.2995 mL | 1.4975 mL | 2.9950 mL | 7.4876 mL |
| 5 mM | 0.0599 mL | 0.2995 mL | 0.5990 mL | 1.4975 mL | |
| 10 mM | 0.0300 mL | 0.1498 mL | 0.2995 mL | 0.7488 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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