PAMP-12 (unmodified) TFA
Based on 2 publication(s) in Google Scholar
PAMP-12 (unmodified) TFA is an endogenous peptide and is a MrgX2 agonist. PAMP-12 (unmodified) TFA can reduce cAMP accumulation, increase Ca2+ levels, enhance beta-arrestin recruitment, decrease IP-1, and increases phosphoERK. PAMP-12 (unmodified) TFA can elicit hypotension through inhibiting catecholamine secretion from sympathetic nerve endings and adrenal chromaffin cells. PAMP-12 (unmodified) TFA can be used for the research of hypotension and ulcerative colitis.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Formel: C79H119F3N24O17
- Molecular Weight:1733.94
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) PAMP-12 (unmodified) TFA
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Biologische Aktivität
Beschreibung
In Vitro
PAMP-12 (10-9-10-6 M; 30 min) TFA potently inhibits Forskolin (HY-15371)-elevated cAMP accumulation in CHO-MrgX2 cells with an EC50 of 57.2 nM[1].
PAMP-12 (10-10-10-6 M) TFA potently induces intracellular Ca2+ mobilization in CHO-MrgX2 cells with an EC50 of 41.0 nM[1].
PAMP-12 (10-10-10-6 M; 60 min) TFA directly binds to and activates MrgX2 in HEK293 cell membranes, stimulating [35S]GTPγS binding with an EC50 of 20.8 nM[1].
PAMP-12 (90 min) TFA stimulation induces greater β-arrestin recruitment to the MRGPRX2 62Ser variant[2].
PAMP-12 (10-7-10-4 M; 2 h) TFA stimulation results in greater IP-1 accumulation, indicating stronger Gq/phospholipase C pathway activation, in CHO cells expressing the MRGPRX2 Asn62 variant compared to the 62Ser variant[2].
PAMP-12 (10-20 μM; 0.5-5 min) TFA stimulation induces greater pERK activation in HMC-1.1 cells expressing the MRGPRX262Ser variant compared to the Asn62 variant[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HMC-1.1 human mast cells stably expressing MRGPRX2 Asn62 or 62Ser variants
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Concentration:10 μM; 20 μM
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Incubation Time:0.5, 1, 5 minutes
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Result:Reached maximal pERK activation at 0.5 and 1 minutes for both variants.
Showed significantly higher normalized pERK levels in the 62Ser variant compared to the Asn62 variant at 1 minute post-stimulation for both 10 μM and 20 μM concentrations.
Chemical Information
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Molecular Weight 1733.94
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Formel C79H119F3N24O17
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Sequence
Phe-Arg-Lys-Lys-Trp-Asn-Lys-Trp-Ala-Leu-Ser-Arg
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Sequence Shortening
FRKKWNKWALSR
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Apigenin ameliorates inflamed ulcerative colitis by regulating mast cell degranulation via the PAMP-MRGPRX2 feedback loop. [Abstract]2025 May:140:156564. PMID: 40054174 -
Cells
Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]2026 Feb 3;15(3):287. PMID: 41677650
Reinheit & Dokumentation
Verweise
[1]. Kamohara M, et al. Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem Biophys Res Commun. 2005;330(4):1146-1152. [Content Brief]
[2]. Chen E, et al. Inflamed Ulcerative Colitis Regions Associated With MRGPRX2-Mediated Mast Cell Degranulation and Cell Activation Modules, Defining a New Therapeutic Target. Gastroenterology. 2021;160(5):1709-1724. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
PAMP-12 (unmodified) TFA Related Classifications
HY-P3419A Related Classifications
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