PAMP-12 (unmodified)
Based on 2 publication(s) in Google Scholar
PAMP-12 (unmodified) is an endogenous peptide and is a MrgX2 agonist. PAMP-12 (unmodified) can reduce cAMP accumulation, increase Ca2+ levels, enhance beta-arrestin recruitment, decrease IP-1, and increases phosphoERK. PAMP-12 (unmodified) can elicit hypotension through inhibiting catecholamine secretion from sympathetic nerve endings and adrenal chromaffin cells. PAMP-12 (unmodified) can be used for the research of hypotension and ulcerative colitis.
For research use only. We do not sell to patients.
- Purity: 99.40%
- CAS No.: 929905-12-4
- Formula: C77H118N24O15
- Molecular Weight:1619.91
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) PAMP-12 (unmodified)
MoreAll Calcium Channel Isoforms
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Biological Activity
PAMP-12 (10-9-10-6 M; 30 min) potently inhibits Forskolin (HY-15371)-elevated cAMP accumulation in CHO-MrgX2 cells with an EC50 of 57.2 nM[1].
PAMP-12 (10-10-10-6 M) potently induces intracellular Ca2+ mobilization in CHO-MrgX2 cells with an EC50 of 41.0 nM[1].
PAMP-12 (10-10-10-6 M; 60 min) directly binds to and activates MrgX2 in HEK293 cell membranes, stimulating [35S]GTPγS binding with an EC50 of 20.8 nM[1].
PAMP-12 (90 min) stimulation induces greater β-arrestin recruitment to the MRGPRX2 62Ser variant[2].
PAMP-12 (10-7-10-4 M; 2 h) stimulation results in greater IP-1 accumulation, indicating stronger Gq/phospholipase C pathway activation, in CHO cells expressing the MRGPRX2 Asn62 variant compared to the 62Ser variant[2].
PAMP-12 (10-20 μM; 0.5-5 min) stimulation induces greater pERK activation in HMC-1.1 cells expressing the MRGPRX262Ser variant compared to the Asn62 variant[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HMC-1.1 human mast cells stably expressing MRGPRX2 Asn62 or 62Ser variants
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Concentration:10 μM; 20 μM
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Incubation Time:0.5, 1, 5 minutes
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Result:Reached maximal pERK activation at 0.5 and 1 minutes for both variants.
Showed significantly higher normalized pERK levels in the 62Ser variant compared to the Asn62 variant at 1 minute post-stimulation for both 10 μM and 20 μM concentrations.
Chemical Information
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CAS No. 929905-12-4
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Appearance Solid
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Molecular Weight 1619.91
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Formula C77H118N24O15
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Color White to off-white
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Sequence
Phe-Arg-Lys-Lys-Trp-Asn-Lys-Trp-Ala-Leu-Ser-Arg
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Sequence Shortening
FRKKWNKWALSR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (2)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Apigenin ameliorates inflamed ulcerative colitis by regulating mast cell degranulation via the PAMP-MRGPRX2 feedback loop. [Abstract]2025 May:140:156564. PMID: 40054174 -
Cells
Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]2026 Feb 3;15(3):287. PMID: 41677650
Solvent & Solubility
H2O : 100 mg/mL (61.73 mM; Need ultrasonic)
DMSO : ≥ 50 mg/mL (30.87 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.6173 mL | 3.0866 mL | 6.1732 mL | 15.4330 mL |
| 5 mM | 0.1235 mL | 0.6173 mL | 1.2346 mL | 3.0866 mL | |
| 10 mM | 0.0617 mL | 0.3087 mL | 0.6173 mL | 1.5433 mL | |
| 15 mM | 0.0412 mL | 0.2058 mL | 0.4115 mL | 1.0289 mL | |
| 20 mM | 0.0309 mL | 0.1543 mL | 0.3087 mL | 0.7716 mL | |
| 25 mM | 0.0247 mL | 0.1235 mL | 0.2469 mL | 0.6173 mL | |
| 30 mM | 0.0206 mL | 0.1029 mL | 0.2058 mL | 0.5144 mL | |
| H2O | 40 mM | 0.0154 mL | 0.0772 mL | 0.1543 mL | 0.3858 mL |
| 50 mM | 0.0123 mL | 0.0617 mL | 0.1235 mL | 0.3087 mL | |
| 60 mM | 0.0103 mL | 0.0514 mL | 0.1029 mL | 0.2572 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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