2. GPCR/G Protein Cell Cycle/DNA Damage Neuronal Signaling Membrane Transporter/Ion Channel
  3. Mas-related G-protein-coupled Receptor (MRGPR) PERK Calcium Channel
  4. PAMP-12 (unmodified) TFA

PAMP-12 (unmodified) TFA is an endogenous peptide and is a MrgX2 agonist. PAMP-12 (unmodified) TFA can reduce cAMP accumulation, increase Ca2+ levels, enhance beta-arrestin recruitment, decrease IP-1, and increases phosphoERK. PAMP-12 (unmodified) TFA can elicit hypotension through inhibiting catecholamine secretion from sympathetic nerve endings and adrenal chromaffin cells. PAMP-12 (unmodified) TFA can be used for the research of hypotension and ulcerative colitis.

For research use only. We do not sell to patients.

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PAMP-12 (unmodified) TFA

PAMP-12 (unmodified) TFA Chemical Structure

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PAMP-12 (unmodified) TFA Related Antibodies

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2 Publications Citing Use of MCE PAMP-12 (unmodified) TFA

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Description

PAMP-12 (unmodified) TFA is an endogenous peptide and is a MrgX2 agonist. PAMP-12 (unmodified) TFA can reduce cAMP accumulation, increase Ca2+ levels, enhance beta-arrestin recruitment, decrease IP-1, and increases phosphoERK. PAMP-12 (unmodified) TFA can elicit hypotension through inhibiting catecholamine secretion from sympathetic nerve endings and adrenal chromaffin cells. PAMP-12 (unmodified) TFA can be used for the research of hypotension and ulcerative colitis[1][2].

In Vitro

PAMP-12 (10-9-10-6 M; 30 min) TFA potently inhibits Forskolin (HY-15371)-elevated cAMP accumulation in CHO-MrgX2 cells with an EC50 of 57.2 nM[1].
PAMP-12 (10-10-10-6 M) TFA potently induces intracellular Ca2+ mobilization in CHO-MrgX2 cells with an EC50 of 41.0 nM[1].
PAMP-12 (10-10-10-6 M; 60 min) TFA directly binds to and activates MrgX2 in HEK293 cell membranes, stimulating [35S]GTPγS binding with an EC50 of 20.8 nM[1].
PAMP-12 (90 min) TFA stimulation induces greater β-arrestin recruitment to the MRGPRX2 62Ser variant[2].
PAMP-12 (10-7-10-4 M; 2 h) TFA stimulation results in greater IP-1 accumulation, indicating stronger Gq/phospholipase C pathway activation, in CHO cells expressing the MRGPRX2 Asn62 variant compared to the 62Ser variant[2].
PAMP-12 (10-20 μM; 0.5-5 min) TFA stimulation induces greater pERK activation in HMC-1.1 cells expressing the MRGPRX262Ser variant compared to the Asn62 variant[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PAMP-12 (unmodified) TFA Related Antibodies

Western Blot Analysis[2]

Cell Line: HMC-1.1 human mast cells stably expressing MRGPRX2 Asn62 or 62Ser variants
Concentration: 10 μM; 20 μM
Incubation Time: 0.5, 1, 5 minutes
Result: Reached maximal pERK activation at 0.5 and 1 minutes for both variants.
Showed significantly higher normalized pERK levels in the 62Ser variant compared to the Asn62 variant at 1 minute post-stimulation for both 10 μM and 20 μM concentrations.
Molecular Weight

1733.94

Formula

C79H119F3N24O17
Sequence

Phe-Arg-Lys-Lys-Trp-Asn-Lys-Trp-Ala-Leu-Ser-Arg
Sequence Shortening

FRKKWNKWALSR
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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PAMP-12 (unmodified) TFA Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PAMP-12 (unmodified)Mas-related G-protein-coupled Receptor (MRGPR)PERKCalcium ChannelProtein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinaseCa2+ channelsCa channelsInhibitorinhibitorinhibit

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