Art. -Nr.: HY-101952G

Prostaglandin E2 (GMP)

(Synonyms: PGE2 (GMP); Dinoprostone (GMP))

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Prostaglandin E2 (GMP) is Prostaglandin E2 (HY-101952) produced by using GMP guidelines. GMP small molecules work appropriately as an auxiliary reagent for cell therapy manufacture. Prostaglandin E2, an inflammatory mediator, is a endogenous hormone-like substance that participate in a wide range of body functions.

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CAS. Nr.: 363-24-6
Formel: C₂₀H₃₂O₅
Molecular Weight:352.47

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Prostaglandin E2 (GMP) Chemical Structure

CAS. Nr.: 363-24-6

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	1.9 nM Compound: Prostaglandin E2	Agonist activity at human EP2 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method Agonist activity at human EP2 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method	[PMID: 26985320]
CHO	EC₅₀	2.5 nM Compound: Prostaglandin E2	Agonist activity at human EP3 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis Agonist activity at human EP3 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	[PMID: 26985320]
CHO	EC₅₀	3.7 nM Compound: Prostaglandin E2	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	[PMID: 26985320]
CHO	EC₅₀	347 nM Compound: Prostaglandin E2	Agonist activity at human IP receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method Agonist activity at human IP receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method	[PMID: 26985320]
CHO	EC₅₀	7.5 nM Compound: Prostaglandin E2	Agonist activity at human EP4 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method Agonist activity at human EP4 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method	[PMID: 26985320]
HEK293	EC₅₀	3 nM Compound: PGE2	EP4 agonist potency utilizing a stable clone of pSV40-EP4 transfected into HEK293 cells expressing EP4 receptor EP4 agonist potency utilizing a stable clone of pSV40-EP4 transfected into HEK293 cells expressing EP4 receptor	[PMID: 12643927]
HEK293	IC₅₀	0.7 nM Compound: PGE2	Inhibitory activity against human EP4 receptor expressed in HEK293 ebna cells Inhibitory activity against human EP4 receptor expressed in HEK293 ebna cells	[PMID: 12643927]
HEK293	EC₅₀	0.2 nM Compound: 1	Agonist activity against rat EP2 receptor expressed in HEK293 cells assessed as stimulation of cAMP release Agonist activity against rat EP2 receptor expressed in HEK293 cells assessed as stimulation of cAMP release	[PMID: 19250823]
HEK293	EC₅₀	0.7 nM Compound: 1	Agonist activity against rat EP4 receptor expressed in HEK293 cells assessed as stimulation of cAMP release Agonist activity against rat EP4 receptor expressed in HEK293 cells assessed as stimulation of cAMP release	[PMID: 19250823]
HEK293	IC₅₀	2.1 nM Compound: 1	Inhibition of rat EP4 receptor expressed in HEK293 cells Inhibition of rat EP4 receptor expressed in HEK293 cells	[PMID: 19250823]
HEK293	IC₅₀	5.2 nM Compound: 1	Inhibition of rat EP2 receptor expressed in HEK293 cells Inhibition of rat EP2 receptor expressed in HEK293 cells	[PMID: 19250823]
HEK293	EC₅₀	346 nM Compound: Prostaglandin E2	Agonist activity at PK2-tagged human EP2 receptor expressed in HEK293 cells assessed as induction of EA-tagged beta-arrestin recruitment incubated for 90 mins by beta-galactosidase reporter gene assay Agonist activity at PK2-tagged human EP2 receptor expressed in HEK293 cells assessed as induction of EA-tagged beta-arrestin recruitment incubated for 90 mins by beta-galactosidase reporter gene assay	[PMID: 26985320]
HEK293	IC₅₀	2.6 nM Compound: PGE2	Displacement of [3H]PGE2 from human recombinant prostanoid EP2 receptor expressed in HEK293 cells Displacement of [3H]PGE2 from human recombinant prostanoid EP2 receptor expressed in HEK293 cells	[PMID: 26988801]
HEK293	IC₅₀	2.6 x 10^-9 M Compound: PGE2	Displacement of [3H]PGE2 from human recombinant EP2 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]PGE2 from human recombinant EP2 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
HEK293	IC₅₀	5.5 x 10^-10 M Compound: PGE2	Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
HEK293	IC₅₀	4 nM Compound: PGE2	Displacement of [3H]prostaglandin E2 from human EP2 receptor expressed in HEK293 cell membranes after 60 mins by liquid scintillation counting Displacement of [3H]prostaglandin E2 from human EP2 receptor expressed in HEK293 cell membranes after 60 mins by liquid scintillation counting	[PMID: 29995405]
K562	IC₅₀	63 μM Compound: 3	In vitro antiproliferative activity was measured on human leukemia K562 cells In vitro antiproliferative activity was measured on human leukemia K562 cells	10.1016/S0960-894X(00)80372-8

In Vitro

Prostaglandin E2 (GMP) (10 nM, supplemented in basic culture medium) blocks Paneth cell formation in cultured human small intestinal organoid (hSIO)^[1].
Prostaglandin E2 (GMP) (1 or 10 μM, 24 h ) increases proliferation of undifferentiated NE-4C stem cells^[2].
Prostaglandin E2 (GMP) (1 μM, 8 day) promotes the progression of NE-4C stem cell differentiation into neuronal-lineage cells^[2].
Prostaglandin E2 (GMP) (1 μM, 6 or 8 day) increases the expression of Cadherin-2 (neurosphere adhesion marker)^[2].
Prostaglandin E2 (GMP) (1 μM) enhances hematopoietic stem cells (HSCs) homing, survival, and proliferation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 363-24-6
Appearance Solid
Molecular Weight 352.47
Formel C₂₀H₃₂O₅
Color White to off-white
SMILES

CCCCC[C@H](O)/C=C/[C@@H]1[C@H](C(C[C@H]1O)=O)C/C=C\CCCC(O)=O
Synonyms

PGE2 (GMP); Dinoprostone (GMP)
Versand

Shipping with dry ice.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (283.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8371 mL

14.1856 mL

28.3712 mL

5 mM

0.5674 mL

2.8371 mL

5.6742 mL

10 mM

0.2837 mL

1.4186 mL

2.8371 mL

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (557 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. He GW, et al. Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation. Cell Stem Cell. 2022 Sep 1;29(9):1333-1345.e6. [Content Brief]

[2]. Wong CT, et al. Prostaglandin E2 promotes neural proliferation and differentiation and regulates Wnt target gene expression. J Neurosci Res. 2016 Aug;94(8):759-75. [Content Brief]

[3]. Hoggatt J, et al. Prostaglandin E2 enhances hematopoietic stem cell homing, survival, and proliferation. Blood. 2009 May 28;113(22):5444-55. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8371 mL	14.1856 mL	28.3712 mL	70.9280 mL
	5 mM	0.5674 mL	2.8371 mL	5.6742 mL	14.1856 mL
	10 mM	0.2837 mL	1.4186 mL	2.8371 mL	7.0928 mL
	15 mM	0.1891 mL	0.9457 mL	1.8914 mL	4.7285 mL
	20 mM	0.1419 mL	0.7093 mL	1.4186 mL	3.5464 mL
	25 mM	0.1135 mL	0.5674 mL	1.1348 mL	2.8371 mL
	30 mM	0.0946 mL	0.4729 mL	0.9457 mL	2.3643 mL
	40 mM	0.0709 mL	0.3546 mL	0.7093 mL	1.7732 mL
	50 mM	0.0567 mL	0.2837 mL	0.5674 mL	1.4186 mL
	60 mM	0.0473 mL	0.2364 mL	0.4729 mL	1.1821 mL
	80 mM	0.0355 mL	0.1773 mL	0.3546 mL	0.8866 mL
	100 mM	0.0284 mL	0.1419 mL	0.2837 mL	0.7093 mL

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Prostaglandin E2 (GMP)

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Prostaglandin E2 (GMP) Related Classifications

HY-101952G Related Classifications