Longanlactone
Longanlactone is a natural pyrrole-lactone alkaloid that can be used as a neurotrophic agent. Longanlactone increases BDNF gene expression in neuro2a cells.
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- CAS No.: 1438721-51-7
- Formule: C13H13NO3
- Masse moléculaire:231.25
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| N2a | IC50 |
121.36 μM
Compound: 1
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Cytotoxicity in mouse Neuro2a cells assessed as reduction in cell viability by MTT assay
Cytotoxicity in mouse Neuro2a cells assessed as reduction in cell viability by MTT assay
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[PMID: 29402744] |
Chemical Information
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CAS No. 1438721-51-7
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Masse moléculaire 231.25
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Formule C13H13NO3
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SMILES
CC(C1=CC=CN1[C@H]2C[C@H](OC2=O)CC#C)=O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Longanlactone Related Classifications
HY-124503 Related Classifications
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