Cat. No.: HY-B1548

Benznidazole

(Synonyms: Benznidazol; Ro 07-1051; Ro 71051)

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Benznidazole (Ro 07-1051) is an antiparasitic medication, with an IC₅₀ of 20.35 μM for Colombian T. cruzi strains, and has been used in the treatment of Chagas disease.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 22994-85-0
Formula: C₁₂H₁₂N₄O₃
Molecular Weight:260.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Benznidazole Chemical Structure

CAS No.: 22994-85-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
- Clinical Trial
Chemical Information
Publications (3)
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- In Vitro
- In Vivo
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Biological Activity

Description

Benznidazole (Ro 07-1051) is an antiparasitic medication, with an IC₅₀ of 20.35 μM for Colombian T. cruzi strains, and has been used in the treatment of Chagas disease^[1]^[2].

IC₅₀ & Target

Trypanosoma

All Parasite Isoforms :

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
3T3	EC₅₀	685.07 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse 3T3 cells after 6 days by beta galactosidase reporter assay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse 3T3 cells after 6 days by beta galactosidase reporter assay	[PMID: 30655955]
786-0	IC₅₀	>100 μM Compound: BZD	Cytotoxicity against human 786-O cells assessed as decrease in mitochondrial viability after 6 hrs by CMXRos staining based assay Cytotoxicity against human 786-O cells assessed as decrease in mitochondrial viability after 6 hrs by CMXRos staining based assay	[PMID: 29407968]
A549	IC₅₀	>100 μM Compound: BZD	Cytotoxicity against human A549 cells after 48 hrs by CellTiter-Glo assay Cytotoxicity against human A549 cells after 48 hrs by CellTiter-Glo assay	[PMID: 29407968]
C2C12	IC₅₀	1.17 μM Compound: BnZ	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi CA-I/72 infected in mouse C2C12 cells measured after 3 days by DAPI staining-based assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi CA-I/72 infected in mouse C2C12 cells measured after 3 days by DAPI staining-based assay	[PMID: 27475533]
C2C12	CC₅₀	>50 μM Compound: Benznidazole	Cytotoxicity against mouse C2C12 cells Cytotoxicity against mouse C2C12 cells	[PMID: 34461718]
C3H	IC₅₀	>100 μM Compound: BZN	Cytotoxicity against mouse C3H cells after 72 hrs by MTS assay Cytotoxicity against mouse C3H cells after 72 hrs by MTS assay	[PMID: 26810263]
C3H	IC₅₀	3 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse C3H cells after 72 hrs by microplate reader analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse C3H cells after 72 hrs by microplate reader analysis	[PMID: 26810263]
Cardiac muscle cell	CC₅₀	>500 μM Compound: Bz	Cytotoxicity against Swiss Webster mouse cardiomyocytes after 72 hrs Cytotoxicity against Swiss Webster mouse cardiomyocytes after 72 hrs	[PMID: 29289882]
Cardiac muscle cell	EC₅₀	>400 μM Compound: Benznidazole	Cytotoxicity against Swiss mouse embryonic cardiomyocytes assessed as reduction in cell viability after 24 hrs by prestoblue-staining based colorimetry Cytotoxicity against Swiss mouse embryonic cardiomyocytes assessed as reduction in cell viability after 24 hrs by prestoblue-staining based colorimetry	[PMID: 30234983]
Cardiac muscle cell	CC₅₀	>500 μM Compound: Bz	Cytotoxicity against mouse cardiomyocytes infected with intracellular amastigote stage of Trypanosoma cruzi Y strain assessed as reduction in cardiomyocyte viability after 72 hrs by Celltiter-Glo assay Cytotoxicity against mouse cardiomyocytes infected with intracellular amastigote stage of Trypanosoma cruzi Y strain assessed as reduction in cardiomyocyte viability after 72 hrs by Celltiter-Glo assay	[PMID: 31434040]
COS-7	CC₅₀	706 μM Compound: Bz	Cytotoxicity against african green monkey COS7 cells after 24 hrs by MTT assay Cytotoxicity against african green monkey COS7 cells after 24 hrs by MTT assay	[PMID: 21664012]
Fibroblast	IC₅₀	>200 μM Compound: BZ	Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 23644203]
Fibroblast	CC₅₀	>200 μM Compound: Benznidazole	Cytotoxicity against mouse NCTC Fibroblast cells assessed as inhibition of cell growth Cytotoxicity against mouse NCTC Fibroblast cells assessed as inhibition of cell growth	[PMID: 36805048]
H9c2	CC₅₀	>50 μM Compound: Benznidazole	Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay	[PMID: 29031078]
H9c2	IC₅₀	>50 μM Compound: Benznidazole	Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis	[PMID: 30100019]
H9c2	EC₅₀	>40 μM Compound: BNZ	Cytotoxicity against rat H9c2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy Cytotoxicity against rat H9c2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy	[PMID: 32551008]
H9c2	EC₅₀	2.2 μM Compound: BNZ	Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in rat H9C2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in rat H9C2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy	[PMID: 32551008]
H9c2	CC₅₀	31.6 μM Compound: Benznidazole	Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability	[PMID: 33479668]
H9c2	IC₅₀	1694.5 μM Compound: Bz	Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability measured after 72 hrs by resazurin due based analysis Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability measured after 72 hrs by resazurin due based analysis	[PMID: 37441850]
HeLa	IC₅₀	25.6 μM Compound: Bz	Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 37 degC after 18 hrs by resazurin assay Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 37 degC after 18 hrs by resazurin assay	[PMID: 23659860]
HeLa	IC₅₀	≥500 μM Compound: Bz	Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 4 degC after 18 hrs by hemocytometric analysis Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 4 degC after 18 hrs by hemocytometric analysis	[PMID: 23659860]
Hepatocyte	CC₅₀	>500 μM Compound: Bz	Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]
HepG2	IC₅₀	2126.16 μM Compound: Benznidazole	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 24630563]
HepG2	IC₅₀	28.1 μM Compound: Bzd	Cytotoxicity against human HepG2 cells after 24 hrs by XTT assay Cytotoxicity against human HepG2 cells after 24 hrs by XTT assay	[PMID: 24929292]
HepG2	GI₅₀	>500 μM Compound: Bnz	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay	[PMID: 31787363]
HFF-1	CC₅₀	>64 μM Compound: 1; BZ	Cytotoxicity against HFF-1 cells incubated for 72 hrs by MTS tetrazolium assay Cytotoxicity against HFF-1 cells incubated for 72 hrs by MTS tetrazolium assay	[PMID: 36057237]
HT-1080	IC₅₀	6.8 μM Compound: BZL	Antitrypanosomal activity against trypomastigotes stage of Trypanosoma cruzi infected in human HT1080 cells assessed as inhibition of parasite growth after 24 hrs by microscopic analysis Antitrypanosomal activity against trypomastigotes stage of Trypanosoma cruzi infected in human HT1080 cells assessed as inhibition of parasite growth after 24 hrs by microscopic analysis	[PMID: 26037321]
HT-1080	CC₅₀	>100 μM Compound: Benznidazole	Cytotoxicity against human HT-1080 cells assessed reduction in cell viability Cytotoxicity against human HT-1080 cells assessed reduction in cell viability	[PMID: 38631020]
J774	IC₅₀	3.6 μM Compound: Bz	Trypanocidal activity against intracellular amastigotes form of Trypanosoma cruzi expressing beta-galactosidase infected in mouse J774 cells on day 7 Trypanocidal activity against intracellular amastigotes form of Trypanosoma cruzi expressing beta-galactosidase infected in mouse J774 cells on day 7	[PMID: 21664012]
J774	IC₅₀	3840.7 μM Compound: Bzn	Cytotoxicity against mouse J774 cells after 18 hrs by MTT assay Cytotoxicity against mouse J774 cells after 18 hrs by MTT assay	[PMID: 22727447]
J774	IC₅₀	>400 μM Compound: BZ	Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay	[PMID: 23816040]
J774	CC₅₀	≥300 μM Compound: B	Cytotoxicity against mouse J774 cells assessed as reduction of cell viability after 24 hrs by resazurin dye-based fluorometric analysis Cytotoxicity against mouse J774 cells assessed as reduction of cell viability after 24 hrs by resazurin dye-based fluorometric analysis	[PMID: 24151871]
J774	CC₅₀	>100 μM Compound: Bdz	Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay	[PMID: 26549870]
J774	CC₅₀	>50 μM Compound: Benznidazole	Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay	[PMID: 29031078]
J774.2	CC₅₀	133.9 μM Compound: Bz	Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay	[PMID: 34571489]
J774.A1	CC₅₀	352.2 μM Compound: Bnz	Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay	[PMID: 30784876]
L6	IC₅₀	0.3 μg/mL Compound: benznidazole	Antitrypanosomal activity against galactosidase gene containing Trypanosoma cruzi Tulahuen C2C4 trypomastigotes in rat L6 cells after 4 days Antitrypanosomal activity against galactosidase gene containing Trypanosoma cruzi Tulahuen C2C4 trypomastigotes in rat L6 cells after 4 days	[PMID: 11087584]
L6	IC₅₀	0.382 μg/mL Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen strain C2C4 containing galactosidase gene in rat L6 cells after 4 days Antitrypanosomal activity against Trypanosoma cruzi Tulahuen strain C2C4 containing galactosidase gene in rat L6 cells after 4 days	[PMID: 12193010]
L6	IC₅₀	0.55 μg/mL Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi in rat L6 cells Antitrypanosomal activity against Trypanosoma cruzi in rat L6 cells	[PMID: 15921410]
L6	IC₅₀	0.74 μg/mL Compound: benznidazol	Antiprotozoal activity against Trypanosoma cruzi Tulahuen C2C4 trypomastigotes in L6 cells Antiprotozoal activity against Trypanosoma cruzi Tulahuen C2C4 trypomastigotes in L6 cells	[PMID: 17253841]
L6	IC₅₀	0.317 μg/mL Compound: Benznidazole	Antiprotozoal activity against Trypanosoma cruzi Tulahuen C4 in rat L6 cells Antiprotozoal activity against Trypanosoma cruzi Tulahuen C4 in rat L6 cells	[PMID: 18683985]
L6	IC₅₀	0.83 μg/mL Compound: benznidazole	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 containing lacZ gene in rat L6 cells after 4 days Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 containing lacZ gene in rat L6 cells after 4 days	[PMID: 19095449]
L6	IC₅₀	1.48 μM Compound: 15	Antitrypanosomal activity against Trypanosoma cruzi amastigotes infected in rat L6 cells Antitrypanosomal activity against Trypanosoma cruzi amastigotes infected in rat L6 cells	[PMID: 19414258]
L6	IC₅₀	>90 μM Compound: Benznidazole	Toxicity against rat L6 cells Toxicity against rat L6 cells	[PMID: 19781948]
L6	IC₅₀	0.521 μM Compound: benznidazole	Antimicrobial activity against Trypanosoma cruzi Tulahuen C2C4 amastigote forms infected in rat L6 cells Antimicrobial activity against Trypanosoma cruzi Tulahuen C2C4 amastigote forms infected in rat L6 cells	[PMID: 19817445]
L6	IC₅₀	1.54 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi infected in rat L6 cells Antitrypanosomal activity against Trypanosoma cruzi infected in rat L6 cells	[PMID: 19942439]
L6	IC₅₀	2.64 μM Compound: Benznidazole	Trypanocidal activity against trypomastigote form of Trypanosoma cruzi Tulahuen infected in rat L6 cells after 96 hrs Trypanocidal activity against trypomastigote form of Trypanosoma cruzi Tulahuen infected in rat L6 cells after 96 hrs	[PMID: 20614907]
L6	IC₅₀	0.489 μg/mL Compound: Benznidazole	Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs	[PMID: 20855214]
L6	IC₅₀	1.35 μM Compound: benznidazole	Antiparasitic activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometrically Antiparasitic activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometrically	[PMID: 21348447]
L6	IC₅₀	2.55 μM Compound: Benznidazole	Antiparasitic activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs Antiparasitic activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs	[PMID: 21937228]
L6	IC₅₀	1.32 μM Compound: Bnz, benznidazole	Antitrypanosomal activity against blood stream form of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells after 72 hrs by alamar blue assay Antitrypanosomal activity against blood stream form of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells after 72 hrs by alamar blue assay	[PMID: 22023653]
L6	IC₅₀	1.134 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing Lac Z infected in rat L6 cells after 96 hrs by spectrophotometry Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing Lac Z infected in rat L6 cells after 96 hrs by spectrophotometry	[PMID: 22061825]
L6	IC₅₀	0.464 μg/mL Compound: Benznidazole	Trypanocidal activity against trypomastigote Trypanosoma cruzi Tulahuen C2C4 infected in L6 cells after 96 hrs by beta-galactosidase reporter gene assay Trypanocidal activity against trypomastigote Trypanosoma cruzi Tulahuen C2C4 infected in L6 cells after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 22217867]
L6	IC₅₀	0.48 μg/mL Compound: benznidazole	Antitrypanosomal activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by spectrophotometric analysis Antitrypanosomal activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by spectrophotometric analysis	[PMID: 22352841]
L6	IC₅₀	0.122 μM Compound: Bzn	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 intracellular amastigotes infected in rat L6 cells after 4 days by photometry Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 intracellular amastigotes infected in rat L6 cells after 4 days by photometry	[PMID: 22727447]
L6	IC₅₀	1.47 μM Compound: Benznidazole	Antiprotozoal activity against intracellular Trypanosoma cruzi amastigotes in rat L6 cells Antiprotozoal activity against intracellular Trypanosoma cruzi amastigotes in rat L6 cells	[PMID: 22932312]
L6	IC₅₀	>384 μM Compound: benznidazole	Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by inverted microscopy Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by inverted microscopy	[PMID: 23153330]
L6	IC₅₀	1.7 μM Compound: benznidazole	Antitrypanosomal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 92 hrs by inverted microscopy Antitrypanosomal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 92 hrs by inverted microscopy	[PMID: 23153330]
L6	IC₅₀	1606 nM Compound: Benzindazole	Antiparasitic activity against Trypanosoma cruzi Tulahuen C2C4 expressing beta-galactosidase infected in rat L6 cells after 96 hrs Antiparasitic activity against Trypanosoma cruzi Tulahuen C2C4 expressing beta-galactosidase infected in rat L6 cells after 96 hrs	[PMID: 23360309]
L6	IC₅₀	>100 μM Compound: Bnz	Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay	[PMID: 23462713]
L6	IC₅₀	1.15 μM Compound: Bnz	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen TcVI extracellular trypomastigotes forms transfected with beta-galactosidase gene infected in rat L6 cells assessed as inhibition of parasite growth after 4 days Antitrypanosomal activity against Trypanosoma cruzi Tulahuen TcVI extracellular trypomastigotes forms transfected with beta-galactosidase gene infected in rat L6 cells assessed as inhibition of parasite growth after 4 days	[PMID: 23462713]
L6	IC₅₀	>150 μM Compound: BNZ	Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay	[PMID: 23795673]
L6	IC₅₀	1.3 μM Compound: BNZ	Antiprotozoal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ infected in rat L6 cells after 48 hrs Antiprotozoal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ infected in rat L6 cells after 48 hrs	[PMID: 23795673]
L6	EC₅₀	1.46 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 23831695]
L6	IC₅₀	1.85 μM Compound: Bnz, Benznidazole	Antimicrobial activity against Trypanosoma cruzi Tulahuen C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay Antimicrobial activity against Trypanosoma cruzi Tulahuen C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 24012457]
L6	IC₅₀	1.671 μM Compound: BNZ	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by photometric analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by photometric analysis	[PMID: 24900706]
L6	IC₅₀	1.5 μM Compound: Bzd	Trypanocidal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 4 days Trypanocidal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 4 days	[PMID: 24929292]
L6	IC₅₀	>150 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells after 3 days by Alamar blue assay Cytotoxicity against rat L6 cells after 3 days by Alamar blue assay	[PMID: 24956553]
L6	EC₅₀	0.38 μg/mL Compound: Benznidazole	Antimicrobial activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by microscopic analysis Antimicrobial activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by microscopic analysis	[PMID: 24978605]
L6	IC₅₀	2.13 μM Compound: BNZ, Rochagan	Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as inhibition of growth after 96 hrs by inverted microscopy Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as inhibition of growth after 96 hrs by inverted microscopy	[PMID: 25240098]
L6	IC₅₀	2.18 μM Compound: Bnz, benznidazole	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by colorimetric analysis Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by colorimetric analysis	[PMID: 25580906]
L6	IC₅₀	>384 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay	[PMID: 26237241]
L6	IC₅₀	1.7 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by inverted microscope analysis Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by inverted microscope analysis	[PMID: 26237241]
L6	IC₅₀	2.153 μM Compound: Bnz, benznidazole	Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by beta-galactosidase reporter gene assay Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 26344593]
L6	IC₅₀	510 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay	[PMID: 26479031]
L6	IC₅₀	1.7 μM Compound: Benznidazole	Antiparasitic activity against Trypanosoma cruzi Tulahuen trypomastigotes infected in rat L6 cells using chromogenic substrate beta-D-galactopyranoside after 96 hrs by beta-galactosidase assay Antiparasitic activity against Trypanosoma cruzi Tulahuen trypomastigotes infected in rat L6 cells using chromogenic substrate beta-D-galactopyranoside after 96 hrs by beta-galactosidase assay	[PMID: 26774924]
L6	IC₅₀	2.1 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric method Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric method	[PMID: 26849852]
L6	IC₅₀	2.206 μM Compound: Benznidazole; Bnz	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as inhibition of parasite growth using CPRG/Nonidet as substrate after 96 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as inhibition of parasite growth using CPRG/Nonidet as substrate after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 27092415]
L6	IC₅₀	>385 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by alamar blue assay Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by alamar blue assay	[PMID: 28284860]
L6	CC₅₀	>385 μM Compound: Bz	Cytotoxicity against rat L6 cels assessed as reduction in cell viability after 72 hrs by Alamar Blue dye-based fluorometric analysis Cytotoxicity against rat L6 cels assessed as reduction in cell viability after 72 hrs by Alamar Blue dye-based fluorometric analysis	[PMID: 30615444]
L6	IC₅₀	>380 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue based inverted microscopy analysis Cytotoxicity against rat L6 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue based inverted microscopy analysis	[PMID: 31784198]
L6	IC₅₀	2.41 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 trypomastigotes infected in rat L6 cells assessed as parasite growth inhibition measured after 96 hrs by inverted microscopy analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 trypomastigotes infected in rat L6 cells assessed as parasite growth inhibition measured after 96 hrs by inverted microscopy analysis	[PMID: 31784198]
L6	IC₅₀	>345 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells assessed as reduction in cell viability after 70 hrs by resazurin dye based inverted microscopic analysis Cytotoxicity against rat L6 cells assessed as reduction in cell viability after 70 hrs by resazurin dye based inverted microscopic analysis	[PMID: 33095014]
L6	IC₅₀	1.7 μM Compound: Benznidazole	Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen infected in L6 cells after 96 hrs by beta-galactosidase reporter assay Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen infected in L6 cells after 96 hrs by beta-galactosidase reporter assay	10.1039/C3MD00065F
L6	IC₅₀	2.325 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 96 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 96 hrs by beta-galactosidase reporter gene assay	10.1039/C4MD00406J
L6	IC₅₀	2.1 μM Compound: Bnz	Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric analysis Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric analysis	10.1039/C5MD00498E
L929	IC₅₀	3.8 μM Compound: Benznidazole	Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis	[PMID: 21940071]
L929	IC₅₀	3.8 μM Compound: Benznidazole	Trypanocidal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse L929 cells assessed as parasite growth inhibition after 7 days by beta-galactosidase assay Trypanocidal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse L929 cells assessed as parasite growth inhibition after 7 days by beta-galactosidase assay	[PMID: 24321832]
L929	CC₅₀	>1000 μM Compound: Benznidazole	Cytotoxicity against mouse L929 cells after 96 hrs by Alamar blue assay Cytotoxicity against mouse L929 cells after 96 hrs by Alamar blue assay	[PMID: 24321832]
L929	CC₅₀	2381 μM Compound: BDZ	Cytotoxicity against mouse L929 cells assessed as cell viability after 96 hrs by alamarBlue assay Cytotoxicity against mouse L929 cells assessed as cell viability after 96 hrs by alamarBlue assay	[PMID: 26231082]
L929	IC₅₀	3.8 μM Compound: BDZ	Antitrypanosomal activity against amastigote and trypomastigote forms of Trypanosoma cruzi Tulahuen expressing beta-galactosidase gene infected in mouse L-929 cells assessed as inhibition of parasite growth after 7 days by colorimetric betagalactosidase a Antitrypanosomal activity against amastigote and trypomastigote forms of Trypanosoma cruzi Tulahuen expressing beta-galactosidase gene infected in mouse L-929 cells assessed as inhibition of parasite growth after 7 days by colorimetric betagalactosidase a	[PMID: 26231082]
L929	IC₅₀	0.68 μM Compound: Benznidazole	Antichagasic activity against Trypanosoma cruzi CL-B5 intracellular amastigotes in mouse L929 cells assessed as growth inhibition after 7 days by beta galactosidase reporter gene assay Antichagasic activity against Trypanosoma cruzi CL-B5 intracellular amastigotes in mouse L929 cells assessed as growth inhibition after 7 days by beta galactosidase reporter gene assay	[PMID: 27017556]
L929	IC₅₀	1.66 μM Compound: Benznidazole	Antichagasic activity against Trypanosoma cruzi Tulahuen amastigotes in mouse L929 cells assessed as growth inhibition after 96 hrs by beta galactosidase reporter gene assay Antichagasic activity against Trypanosoma cruzi Tulahuen amastigotes in mouse L929 cells assessed as growth inhibition after 96 hrs by beta galactosidase reporter gene assay	[PMID: 27017556]
L929	IC₅₀	22.73 μM Compound: Benznidazole	Antichagasic activity against Trypanosoma cruzi CL-B5 epimastigotes in mouse L929 cells assessed as growth inhibition after 72 hrs by beta galactosidase reporter gene assay Antichagasic activity against Trypanosoma cruzi CL-B5 epimastigotes in mouse L929 cells assessed as growth inhibition after 72 hrs by beta galactosidase reporter gene assay	[PMID: 27017556]
L929	CC₅₀	2381 μM Compound: Benznidazole	Cytotoxicity against mouse L929 cells after 96 hrs by alamar blue assay Cytotoxicity against mouse L929 cells after 96 hrs by alamar blue assay	[PMID: 28215783]
L929	IC₅₀	>256 μM Compound: Benznidazol	Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay	[PMID: 28499168]
L929	IC₅₀	>200 μM Compound: BZ	Cytotoxicity against mouse NCTC-929 cells after 72 hrs by MTT assay Cytotoxicity against mouse NCTC-929 cells after 72 hrs by MTT assay	[PMID: 28881289]
L929	CC₅₀	>200 μM Compound: Benznidazole	Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31000155]
L929	CC₅₀	190.6 μM Compound: Benznidazole	Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31103897]
L929	EC₅₀	>200 μM Compound: BZ	Cytotoxicity against mouse L929 cells assessed as reduction in cell viability Cytotoxicity against mouse L929 cells assessed as reduction in cell viability	[PMID: 31495177]
L929	CC₅₀	>200 μM Compound: BZD	Cytotoxicity against mouse NCTC clone 929 cells incubated for 48 hrs by MTT spectrophotometric assay Cytotoxicity against mouse NCTC clone 929 cells incubated for 48 hrs by MTT spectrophotometric assay	[PMID: 36623331]
L929	CC₅₀	>200 μM Compound: Benzn	Cytotoxicity against mouse L929 cells incubated for 72 hrs by fluorescence based microplate reader analysis Cytotoxicity against mouse L929 cells incubated for 72 hrs by fluorescence based microplate reader analysis	[PMID: 37729094]
L929	CC₅₀	2381 μM Compound: Benznidazole	Cytotoxicity against mouse L929 cells incubated for 96 hrs by Alamar blue assay Cytotoxicity against mouse L929 cells incubated for 96 hrs by Alamar blue assay	[PMID: 38677111]
L929	CC₅₀	2113 μM Compound: Benznidazole	Cytotoxicity against mouse L929 cells assessed as inhibition of cell viability measured after 120 hrs Cytotoxicity against mouse L929 cells assessed as inhibition of cell viability measured after 120 hrs	[PMID: 38964519]
LLC-MK2	IC₅₀	36.3 μg/mL Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Y infected in human LLC-MK2 cells after 24 hrs by MTT assay Antitrypanosomal activity against Trypanosoma cruzi Y infected in human LLC-MK2 cells after 24 hrs by MTT assay	[PMID: 18177008]
LLC-MK2	IC₅₀	>1366 μM Compound: Benznidazole	Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 20934347]
LLC-MK2	IC₅₀	172.37 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 20934347]
LLC-MK2	IC₅₀	0.125 mM Compound: Bz	Antitrypanosomal activity against Trypanosoma cruzi Tulaheun infected in monkey LLC-MK2 cells after 4 days by beta-galactosidase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi Tulaheun infected in monkey LLC-MK2 cells after 4 days by beta-galactosidase reporter gene assay	[PMID: 22154559]
LLC-MK2	CC₅₀	614.7 μM Compound: BZ	Cytotoxicity against monkey LLC-MK2 cells after 96 hrs by sulforhodamine B assay Cytotoxicity against monkey LLC-MK2 cells after 96 hrs by sulforhodamine B assay	[PMID: 22440015]
LLC-MK2	EC₅₀	34.5 μM Compound: BZ	Antitrypanosomal activity against Trypanosoma cruzi Y trymastigotes infected in monkey LLC-MK2 cells after 24 hrs by Brenner counter method Antitrypanosomal activity against Trypanosoma cruzi Y trymastigotes infected in monkey LLC-MK2 cells after 24 hrs by Brenner counter method	[PMID: 22440015]
LLC-MK2	IC₅₀	19.2 μM Compound: BZ	Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in monkey LLC-MK2 cells after 96 hrs by Geimsa staining method Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in monkey LLC-MK2 cells after 96 hrs by Geimsa staining method	[PMID: 22440015]
LLC-MK2	IC₅₀	11.3 μM Compound: Bdz	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in rhesus monkey LLC-MK2 cells assessed parasite viability after 24 hrs Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in rhesus monkey LLC-MK2 cells assessed parasite viability after 24 hrs	[PMID: 23006639]
LLC-MK2	IC₅₀	6.2 μM Compound: Bdz	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs	[PMID: 23167554]
LLC-MK2	IC₅₀	6.6 μM Compound: Bdz	Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days	[PMID: 23167554]
LLC-MK2	IC₅₀	0.03 mM Compound: Bz, Rochagan	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen expressing beta galactosidase infected in rhesus monkey LLC-MK2 cells assessed as inhibition of parasite viability treated at 24 hrs post-infection measured after 4 days b Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen expressing beta galactosidase infected in rhesus monkey LLC-MK2 cells assessed as inhibition of parasite viability treated at 24 hrs post-infection measured after 4 days b	[PMID: 23415086]
LLC-MK2	EC₅₀	1.9 μM Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi trypomastigote infected in monkey LLC-MK2 cells after 96 hrs by luciferase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi trypomastigote infected in monkey LLC-MK2 cells after 96 hrs by luciferase reporter gene assay	[PMID: 23448316]
LLC-MK2	CC₅₀	614.7 μM Compound: Benznidazole	Cytotoxicity against monkey LLC-MK2 cells after 48 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 24398381]
LLC-MK2	EC₅₀	34.5 μM Compound: Benznidazole	Antiparasitic activity against trypomastigote form of Trypanosoma cruzi Y infected in LLCMK2 cells assessed as parasite lysis after 24 hrs by Pizzi-Brener method Antiparasitic activity against trypomastigote form of Trypanosoma cruzi Y infected in LLCMK2 cells assessed as parasite lysis after 24 hrs by Pizzi-Brener method	[PMID: 24398381]
LLC-MK2	CC₅₀	6.2 μM Compound: Bdz	Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis	[PMID: 24561675]
LLC-MK2	IC₅₀	6.2 μM Compound: Bdz	Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as reduction in viable parasites after 24 hrs by Neubauer chamber counting method Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as reduction in viable parasites after 24 hrs by Neubauer chamber counting method	[PMID: 25147146]
LLC-MK2	EC₅₀	3.4 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Y infected in monkey LLC-MK2 cells after 24 hrs by Pizzi-Brener method Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Y infected in monkey LLC-MK2 cells after 24 hrs by Pizzi-Brener method	[PMID: 25461316]
LLC-MK2	IC₅₀	477 μM Compound: Benznidazole	Cytotoxicity against rhesus monkey LLC-MK2 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against rhesus monkey LLC-MK2 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 25924111]
LLC-MK2	IC₅₀	16 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Y trypomastigote forms infected in monkey LLC-MK2 cells assessed as reduction in parasite viability after 24 hrs by resazurin assay Antitrypanosomal activity against Trypanosoma cruzi Y trypomastigote forms infected in monkey LLC-MK2 cells assessed as reduction in parasite viability after 24 hrs by resazurin assay	[PMID: 27586460]
LLC-MK2	CC₅₀	>200 μM Compound: BZ	Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33199200]
LLC-MK2	EC₅₀	502.6 μM Compound: Benznidazole	Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 35933786]
LLC-MK2	CC₅₀	>50 μM Compound: 1; BZN	Cytotoxicity against monkey LLC-MK2 cells incubated for 72 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells incubated for 72 hrs by MTT assay	[PMID: 37573209]
LLC-MK2	CC₅₀	>50 μM Compound: BZN	Cytotoxicity against monkey LLC-MK2 cells incubated for 72 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells incubated for 72 hrs by MTT assay	[PMID: 38305199]
Macrophage	IC₅₀	16 μM Compound: Benznidazole	Antiparasitic activity against amastigote form of Trypanosoma cruzi Y strain infected in bone marrow macrophage cells assessed as amastigote viability incubated for 4 days by rapid panoptic stain based microscopy method Antiparasitic activity against amastigote form of Trypanosoma cruzi Y strain infected in bone marrow macrophage cells assessed as amastigote viability incubated for 4 days by rapid panoptic stain based microscopy method	[PMID: 26476667]
Macrophage	CC₅₀	>100 μM Compound: Benznidazole	Cytotoxicity against Balb/c mouse peritoneal macrophage assessed as cell viability measured after 72 hrs by alamar blue assay Cytotoxicity against Balb/c mouse peritoneal macrophage assessed as cell viability measured after 72 hrs by alamar blue assay	[PMID: 32902988]
MRC5	CC₅₀	>50 μM Compound: Benznidazole	In Vitro cytotoxicity on MRC-5 cells In Vitro cytotoxicity on MRC-5 cells	[PMID: 10893302]
MRC5	CC₅₀	>50 μM Compound: Benznidazole	Tested for cytotoxicity towards human MRC-5 cells Tested for cytotoxicity towards human MRC-5 cells	[PMID: 12443785]
MRC5	IC₅₀	2.56 μM Compound: Benznidazole	Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay	[PMID: 25199582]
MRC5	IC₅₀	1.95 μM Compound: BNZ	Antiparasitic activity against nifurtimox-sensitive amastigote stage of Trypanosoma cruzi tulahuen CL2 infected in human MRC5 cells Antiparasitic activity against nifurtimox-sensitive amastigote stage of Trypanosoma cruzi tulahuen CL2 infected in human MRC5 cells	[PMID: 26922226]
MRC5	EC₅₀	>64 μM Compound: Benznidazole	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay	[PMID: 30677668]
MRC5	IC₅₀	47.2 μM Compound: Bzn	Cytotoxicity against human MRC5 cells Cytotoxicity against human MRC5 cells	[PMID: 38971047]
NIH3T3	ED₅₀	>50 μM Compound: benznidazole	Effective dose required against Trypanosoma cruzi amastigotes in 3T3 Fibroblasts Effective dose required against Trypanosoma cruzi amastigotes in 3T3 Fibroblasts	[PMID: 11206479]
NIH3T3	ED₅₀	>50 μM Compound: Benznidazole	Effective concentration required to inhibit the growth of murine 3T3 fibroblasts Effective concentration required to inhibit the growth of murine 3T3 fibroblasts	[PMID: 11405646]
NIH3T3	EC₅₀	>50 μM Compound: benznidazole	Cytotoxicity against mouse NIH/3T3 cells Cytotoxicity against mouse NIH/3T3 cells	[PMID: 23448316]
NIH3T3	CC₅₀	>127 μM Compound: 1; BZ	Cytotoxicity against mouse 3T3 cells Cytotoxicity against mouse 3T3 cells	[PMID: 36111399]
Peritoneal macrophage	IC₅₀	1141 μM Compound: benznidazole	Cytotoxicity against Balb/c mouse peritoneal macrophages after 24 hrs by MTT assay Cytotoxicity against Balb/c mouse peritoneal macrophages after 24 hrs by MTT assay	[PMID: 22559947]
RAW264.7	CC₅₀	137.54 μM Compound: Bnz	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30996772]
RAW264.7	GI₅₀	>500 μM Compound: Bnz	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay	[PMID: 31787363]
RAW264.7	CC₅₀	123.7 μM Compound: BZN	Cytotoxicity against mouse RAW264.7 cells incubated for 48 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells incubated for 48 hrs by MTT assay	[PMID: 37062170]
RAW264.7	CC₅₀	44.18 μM Compound: BZN	Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 37267753]
THP-1	CC₅₀	>125.4 μM Compound: Benznidazole	Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 26906638]
THP-1	IC₅₀	10.18 μM Compound: Benznidazole	Antiprotozoan activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen infected in human THP1 cells assessed as reduction in parasite viability after 48 hrs by microplate based assay Antiprotozoan activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen infected in human THP1 cells assessed as reduction in parasite viability after 48 hrs by microplate based assay	[PMID: 26906638]
THP-1	CC₅₀	>500 μM Compound: BZN	Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28152426]
THP-1	CC₅₀	>500 μM Compound: Bnz	Cytotoxicity against human THP1 cells after 72 hrs by MTT assay Cytotoxicity against human THP1 cells after 72 hrs by MTT assay	[PMID: 30173929]
THP-1	CC₅₀	>200 μM Compound: Benznidazole	Cytotoxicity against human THP-1 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human THP-1 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32652409]
THP-1	IC₅₀	>100 μM Compound: Bzn	Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin staining based analysis Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin staining based analysis	[PMID: 37859713]
U2OS	CC₅₀	>50 μM Compound: Benznidazole	Cytotoxicity against human U2OS cells infected with intracellular Trypanosoma cruzi Cytotoxicity against human U2OS cells infected with intracellular Trypanosoma cruzi	[PMID: 25036797]
U2OS	EC₅₀	20.7 μM Compound: Benznidazole	Antitrypanosomal activity against intracellular Trypanosoma cruzi infected in human U2OS cells after 48 hrs by confocal microscopy Antitrypanosomal activity against intracellular Trypanosoma cruzi infected in human U2OS cells after 48 hrs by confocal microscopy	[PMID: 25036797]
U2OS	EC₅₀	3.96 μM Compound: Benznidazole	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay	[PMID: 27318979]
U2OS	CC₅₀	>200 μM Compound: BZD	Cytotoxicity against human U2OS cells assessed as reduction in cell viability Cytotoxicity against human U2OS cells assessed as reduction in cell viability	[PMID: 29247858]
U2OS	CC₅₀	>200 μM Compound: Benznidazole	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 96 hrs Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 96 hrs	[PMID: 30562700]
U-937	EC₅₀	10.7 μg/mL Compound: Benz	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as parasite growth inhibition after 72 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as parasite growth inhibition after 72 hrs by beta-galactosidase reporter gene assay	[PMID: 25725376]
U-937	EC₅₀	10.5 μg/mL Compound: Benznidazole	Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as reduction of viability of intracellular amastigotes by measuring beta-galactosidase activity after 72 hrs by spectrophotometry a Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as reduction of viability of intracellular amastigotes by measuring beta-galactosidase activity after 72 hrs by spectrophotometry a	[PMID: 26218652]
V79	IC₅₀	2 mM Compound: 2, benznidazole	Cytotoxicity against chinese hamster V79 cells Cytotoxicity against chinese hamster V79 cells	[PMID: 18570370]
Vero	IC₅₀	3.53 μg/mL Compound: benznidazole	Toxicity against Vero cells after 72 hrs Toxicity against Vero cells after 72 hrs	[PMID: 17222558]
Vero	IC₅₀	14.58 μM Compound: benznidazol	Antiprotozoal activity against Trypanosoma cruzi Y in vero cells Antiprotozoal activity against Trypanosoma cruzi Y in vero cells	[PMID: 17889546]
Vero	CC₅₀	>1000 μM Compound: benznidazol	Cytotoxicity against Vero cells by MTT assay Cytotoxicity against Vero cells by MTT assay	[PMID: 17889546]
Vero	IC₅₀	13.6 μM Compound: benznidazole	Toxicity in african green monkey Vero cells after 72 hrs Toxicity in african green monkey Vero cells after 72 hrs	[PMID: 18293910]
Vero	IC₅₀	13.56 μM Compound: BZN	Cytotoxicity against african green monkey Vero cells after 72 hrs by trypan blue assay Cytotoxicity against african green monkey Vero cells after 72 hrs by trypan blue assay	[PMID: 21229977]
Vero	CC₅₀	14 μM Compound: Benznidazole	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21397502]
Vero	IC₅₀	2.768 μM Compound: 2	Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition by fluorescence assay Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition by fluorescence assay	[PMID: 21419634]
Vero	IC₅₀	13.6 μM Compound: benznidazole	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis	[PMID: 21466157]
Vero	IC₅₀	2.1 μM Compound: BNZ	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen 2 infected in african green monkey Vero cells expressing red fluorescent protein after 3 days by fluorescence analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen 2 infected in african green monkey Vero cells expressing red fluorescent protein after 3 days by fluorescence analysis	[PMID: 22414614]
Vero	IC₅₀	2.1 μM Compound: BNZ	Cytotoxicity against african green monkey Vero cells after 3 days Cytotoxicity against african green monkey Vero cells after 3 days	[PMID: 22414614]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by flow cytometric analysis Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by flow cytometric analysis	[PMID: 22443115]
Vero	IC₅₀	23.3 μM Compound: BZN	Trypanosomicidal activity against Trypanosoma cruzi SN3 amastigote infected in Vero cells after 72 hrs by Giemsa staining method Trypanosomicidal activity against Trypanosoma cruzi SN3 amastigote infected in Vero cells after 72 hrs by Giemsa staining method	[PMID: 22443115]
Vero	CC₅₀	48 μM Compound: Benznidazole	Cytotoxicity against african green monkey Vero cells after 72 hrs by spectrophotometric analysis Cytotoxicity against african green monkey Vero cells after 72 hrs by spectrophotometric analysis	[PMID: 22795899]
Vero	CC₅₀	82.79 μM Compound: Bnz	Cytotoxicity against african green monkey Vero cells after 24 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 24 hrs by MTT assay	[PMID: 22840495]
Vero	IC₅₀	13.6 μM Compound: BZN	Toxicity against african green monkey Vero cells Toxicity against african green monkey Vero cells	[PMID: 23043291]
Vero	IC₅₀	15.9 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 epimastigotes infected in african green monkey Vero cells incubated for 72 hrs Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 epimastigotes infected in african green monkey Vero cells incubated for 72 hrs	[PMID: 23043291]
Vero	IC₅₀	18.9 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 axenic amastigotes infected in african green monkey Vero cells incubated for 72 hrs Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 axenic amastigotes infected in african green monkey Vero cells incubated for 72 hrs	[PMID: 23043291]
Vero	IC₅₀	23.3 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 intracellular amastigotes infected in african green monkey Vero cells incubated for 72 hrs Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 intracellular amastigotes infected in african green monkey Vero cells incubated for 72 hrs	[PMID: 23043291]
Vero	ED₅₀	1.7 μM Compound: Benznidazole	Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition measured after 3 days by fluorescence assay Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition measured after 3 days by fluorescence assay	[PMID: 23318904]
Vero	IC₅₀	13.6 μM Compound: Bzn	Cytotoxicity against African green monkey Vero cells after 72 hrs by flow cytometric analysis Cytotoxicity against African green monkey Vero cells after 72 hrs by flow cytometric analysis	[PMID: 24158012]
Vero	IC₅₀	23 μM Compound: Bzn	Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells after 72 hrs by Giemsa staining assay Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells after 72 hrs by Giemsa staining assay	[PMID: 24158012]
Vero	ED₅₀	1.44 μM Compound: Benznidazole	Antimicrobial activity against Trypanosoma cruzi CL amastigotes infected in african green monkey Vero cells measured on day 3 by fluorescence assay Antimicrobial activity against Trypanosoma cruzi CL amastigotes infected in african green monkey Vero cells measured on day 3 by fluorescence assay	[PMID: 24300918]
Vero	IC₅₀	13.6 μM Compound: BNZ	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide/fluorescein diacetate staining-based flow cytometric analysis Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide/fluorescein diacetate staining-based flow cytometric analysis	[PMID: 24410674]
Vero	IC₅₀	23.3 μM Compound: BNZ	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells assessed as parasite growth inhibition after 72 hrs by Giemsa staining-based microscopic analysis Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells assessed as parasite growth inhibition after 72 hrs by Giemsa staining-based microscopic analysis	[PMID: 24410674]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide staining-based flow cytometric analysis Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide staining-based flow cytometric analysis	[PMID: 24448422]
Vero	IC₅₀	23.3 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi (IRHOD/CO/2008/SN3) amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa staining-based microscopic analysis Antitrypanosomal activity against Trypanosoma cruzi (IRHOD/CO/2008/SN3) amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa staining-based microscopic analysis	[PMID: 24448422]
Vero	CC₅₀	14 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay	[PMID: 24529307]
Vero	IC₅₀	3.3 μM Compound: Bnz	Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay	[PMID: 24749923]
Vero	IC₅₀	13.6 μM Compound: Bz	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 25462275]
Vero	IC₅₀	23.3 μM Compound: Bz	Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi IRHOD/CO/2008/SN3 in african green monkey Vero cells Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi IRHOD/CO/2008/SN3 in african green monkey Vero cells	[PMID: 25462275]
Vero	IC₅₀	6.3 μM Compound: Bdz	Trypanocidal activity against trypamostigote form of Trypanosoma cruzi Y infected in African green monkey Vero cells after 24 hrs by neubauer chamber method Trypanocidal activity against trypamostigote form of Trypanosoma cruzi Y infected in African green monkey Vero cells after 24 hrs by neubauer chamber method	[PMID: 26854377]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity in African green monkey Vero cells incubated for 72 hrs Cytotoxicity in African green monkey Vero cells incubated for 72 hrs	[PMID: 28002965]
Vero	CC₅₀	14 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay	[PMID: 28645659]
Vero	CC₅₀	365.71 μM Compound: Bz	Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]
Vero	IC₅₀	23.2 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 29883536]
Vero	IC₅₀	30.17 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 30344907]
Vero	IC₅₀	10 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi TINF/CH/1956/Tulahuen DTU 6 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay Trypanocidal activity against Trypanosoma cruzi TINF/CH/1956/Tulahuen DTU 6 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay	[PMID: 30583247]
Vero	IC₅₀	15.1 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells assessed as reduction in infected cells after 72 hrs by Giemsa-staining based assay Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells assessed as reduction in infected cells after 72 hrs by Giemsa-staining based assay	[PMID: 30583247]
Vero	IC₅₀	16.6 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 DTU 1 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay Trypanocidal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 DTU 1 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay	[PMID: 30583247]
Vero	IC₅₀	23.2 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 to 72 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 to 72 hrs by resazurin dye based assay	[PMID: 30583247]
Vero	IC₅₀	8.3 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay	[PMID: 30583247]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells after 72 hrs by propidium iodide/fluorescein diacetate staining based flow cytometry Cytotoxicity against African green monkey Vero cells after 72 hrs by propidium iodide/fluorescein diacetate staining based flow cytometry	[PMID: 30641409]
Vero	IC₅₀	23.3 μM Compound: BZN	Anti-trypanosomal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic analysis Anti-trypanosomal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic analysis	[PMID: 30641409]
Vero	IC₅₀	10 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi TINF/CH/1956/Tulahuen DTU 6 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay Trypanocidal activity against Trypanosoma cruzi TINF/CH/1956/Tulahuen DTU 6 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay	[PMID: 30728107]
Vero	IC₅₀	15.1 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells assessed as reduction in infected cells after 72 hrs by Giemsa-staining based assay Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells assessed as reduction in infected cells after 72 hrs by Giemsa-staining based assay	[PMID: 30728107]
Vero	IC₅₀	16.6 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 DTU 1 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay Trypanocidal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 DTU 1 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay	[PMID: 30728107]
Vero	IC₅₀	23.2 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 30728107]
Vero	IC₅₀	8.3 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay Trypanocidal activity against Trypanosoma cruzi MHOM/Pe/2011/Arequipa DTU 5 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic assay	[PMID: 30728107]
Vero	ED₅₀	>10 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells after 72 hrs by Alamar Blue assay Cytotoxicity against African green monkey Vero cells after 72 hrs by Alamar Blue assay	[PMID: 30808607]
Vero	ED₅₀	2.48 μM Compound: Benznidazole	Antitrypanosomal activity against tdTomato red expressing Trypanosoma cruzi CL amastigotes infected in gamma-irradiated African green monkey Vero cells after 72 hrs by fluorescence assay Antitrypanosomal activity against tdTomato red expressing Trypanosoma cruzi CL amastigotes infected in gamma-irradiated African green monkey Vero cells after 72 hrs by fluorescence assay	[PMID: 30808607]
Vero	CC₅₀	>500 μM Compound: Bnz	Cytotoxicity against African green monkey Vero cells 72 hrs by CellTiter-Glo assay Cytotoxicity against African green monkey Vero cells 72 hrs by CellTiter-Glo assay	[PMID: 30996772]
Vero	IC₅₀	1.7 μM Compound: Bnz	Antitrypanosomal activity against Trypanosoma cruzi Dm28c intracellular amastigotes infected in African green monkey Vero cells incubated for 24 hrs by luciferase-luminescence based assay Antitrypanosomal activity against Trypanosoma cruzi Dm28c intracellular amastigotes infected in African green monkey Vero cells incubated for 24 hrs by luciferase-luminescence based assay	[PMID: 30996772]
Vero	IC₅₀	15.6 μM Compound: Bnz	Antitrypanosomal activity against Trypanosoma cruzi Dm28c trypomastigotes infected in African green monkey Vero cells incubated for 24 hrs by luciferase-luminescence based assay Antitrypanosomal activity against Trypanosoma cruzi Dm28c trypomastigotes infected in African green monkey Vero cells incubated for 24 hrs by luciferase-luminescence based assay	[PMID: 30996772]
Vero	ED₅₀	2.58 μM Compound: Benznidazole	Antiparasitic activity against Trypanosoma cruzi amastigote infected in African green monkey Vero cells decrease in parasite viability measured at day 3 of post infection by red fluorescent protein based fluorescence method Antiparasitic activity against Trypanosoma cruzi amastigote infected in African green monkey Vero cells decrease in parasite viability measured at day 3 of post infection by red fluorescent protein based fluorescence method	[PMID: 31296439]
Vero	IC₅₀	>200 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells measured after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells measured after 72 hrs by MTT assay	[PMID: 31345745]
Vero	IC₅₀	4.1 μM Compound: BZN	Trypanocidal activity against Trypanosoma cruzi trypomastigote form infected in African green monkey Vero cells measured after 48 hrs by resazurin dye based assay Trypanocidal activity against Trypanosoma cruzi trypomastigote form infected in African green monkey Vero cells measured after 48 hrs by resazurin dye based assay	[PMID: 31345745]
Vero	CC₅₀	>500 μM Compound: Bz	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability by measuring ATP level after 72 hrs by Celltiter-Glo assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability by measuring ATP level after 72 hrs by Celltiter-Glo assay	[PMID: 31434040]
Vero	IC₅₀	1.9 μM Compound: Bz	Trypanocidal activity against intracellular amastigote stage of luciferase expressing Trypanosoma cruzi Dm28c infected in Vero cells assessed as reduction in parasite viability after 72 hrs in presence of luciferin by Giemsa staining based optical microsc Trypanocidal activity against intracellular amastigote stage of luciferase expressing Trypanosoma cruzi Dm28c infected in Vero cells assessed as reduction in parasite viability after 72 hrs in presence of luciferin by Giemsa staining based optical microsc	[PMID: 31434040]
Vero	IC₅₀	19.1 μM Compound: Bz	Trypanocidal activity against luciferase expressing Trypanosoma cruzi Dm28c trypomastigotes isolated from infected Vero cells assessed as reduction in parasite viability after 24 hrs by luciferase-luminescence assay Trypanocidal activity against luciferase expressing Trypanosoma cruzi Dm28c trypomastigotes isolated from infected Vero cells assessed as reduction in parasite viability after 24 hrs by luciferase-luminescence assay	[PMID: 31434040]
Vero	CC₅₀	706 μM Compound: BNZ	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 31771829]
Vero	IC₅₀	3.6 μM Compound: BNZ	Antitrypanosomal activity against bloodstream Trypanosoma cruzi amastigotes expressing beta-galactosidase infected in african green monkey VERO cells incubated for 5 days measured by microplate reader assay Antitrypanosomal activity against bloodstream Trypanosoma cruzi amastigotes expressing beta-galactosidase infected in african green monkey VERO cells incubated for 5 days measured by microplate reader assay	[PMID: 31771829]
Vero	EC₅₀	0.5 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi CL Brener amastigotes infected in African green monkey Vero cells assessed as reduction in parasite growth incubated for 120 hrs at 24 hrs post-infection and measured on day 6 post-infection by hemocytom Antitrypanosomal activity against Trypanosoma cruzi CL Brener amastigotes infected in African green monkey Vero cells assessed as reduction in parasite growth incubated for 120 hrs at 24 hrs post-infection and measured on day 6 post-infection by hemocytom	[PMID: 32292551]
Vero	IC₅₀	80.4 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by resazurin dye based assay	[PMID: 33253573]
Vero	IC₅₀	>100 μM Compound: Bzn	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin staining based analysis Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin staining based analysis	[PMID: 37859713]
Vero	CC₅₀	>384 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells incubated for 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells incubated for 48 hrs by MTT assay	[PMID: 38894931]
Vero	CC₅₀	309.1 μM Compound: Bnz	Cytotoxicity against African green monkey Vero cells after 72 hrs by resazurin assay Cytotoxicity against African green monkey Vero cells after 72 hrs by resazurin assay	10.1039/C2MD00283C
Vero	IC₅₀	12.5 μM Compound: Bnz	Antitrypanosomal activity against epimastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells after 72 hrs relative to untreated control Antitrypanosomal activity against epimastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells after 72 hrs relative to untreated control	10.1039/C2MD00283C

In Vivo

Benznidazole (100 mg/kg/day, p.o., 30 days) produces a decrease in electrocardiographic alterations, fewer modifications in the affinity and density of cardiac-receptors, and few isolated areas of fibrosis in the heart, in mice infected with Trypanosoma cruzi Tulahuen strain or SGO-Z12 isolate and treated at 180 days post infection (p.i.) (i.e. chronic phase) with Benznidazole^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mice infected with Trypanosoma cruzi Tulahuen strain or SGO-Z12^[1]
Dosage:

100 mg/kg/day
Administration:

Orally for 30 days
Result:

Produced a decrease in electrocardiographic alterations, fewer modifications in the affinity and density of cardiac-receptors, and few isolated areas of fibrosis in the heart.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 22994-85-0
Appearance Solid
Molecular Weight 260.25
Formula C₁₂H₁₂N₄O₃
Color Off-white to light yellow
SMILES

O=C(NCC1=CC=CC=C1)CN2C=CN=C2[N+]([O-])=O
Synonyms

Benznidazol; Ro 07-1051; Ro 71051
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Nat Commun

Localized cardiac small molecule trajectories and persistent chemical sequelae in experimental Chagas disease. [Abstract]

2023 Oct 25;14(1):6769. PMID: 37880260
bioRxiv

The impact of vaccine-linked chemotherapy on liver health in a mouse model of chronic Trypanosoma cruzi infection. [Abstract]

2023 Jul 12:2023.07.11.548497. PMID: 37503013
Res Sq

Localized cardiac metabolic trajectories and post-infectious metabolic sequelae in experimental Chagas disease. [Abstract]

2023 Jan 20:rs.3.rs-2497474. PMID: 36711878

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (192.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8425 mL

19.2123 mL

38.4246 mL

5 mM

0.7685 mL

3.8425 mL

7.6849 mL

10 mM

0.3842 mL

1.9212 mL

3.8425 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg
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g
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μL
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
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- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bustamante JM, et al. Treatment with benznidazole or thioridazine in the chronic phase of experimental Chagas disease improves cardiopathy. Int J Antimicrob Agents. 2007 Jun;29(6):733-7. [Content Brief]

[2]. Luna KP, et al. In vitro susceptibility of Trypanosoma cruzi strains from Santander, Colombia, to hexadecylphosphocholine (miltefosine), nifurtimox and benznidazole. Biomedica. 2009 Sep;29(3):448-55. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8425 mL	19.2123 mL	38.4246 mL	96.0615 mL
	5 mM	0.7685 mL	3.8425 mL	7.6849 mL	19.2123 mL
	10 mM	0.3842 mL	1.9212 mL	3.8425 mL	9.6061 mL
	15 mM	0.2562 mL	1.2808 mL	2.5616 mL	6.4041 mL
	20 mM	0.1921 mL	0.9606 mL	1.9212 mL	4.8031 mL
	25 mM	0.1537 mL	0.7685 mL	1.5370 mL	3.8425 mL
	30 mM	0.1281 mL	0.6404 mL	1.2808 mL	3.2020 mL
	40 mM	0.0961 mL	0.4803 mL	0.9606 mL	2.4015 mL
	50 mM	0.0768 mL	0.3842 mL	0.7685 mL	1.9212 mL
	60 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6010 mL
	80 mM	0.0480 mL	0.2402 mL	0.4803 mL	1.2008 mL
	100 mM	0.0384 mL	0.1921 mL	0.3842 mL	0.9606 mL

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Benznidazole

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Publications Citing Use of MedChemExpress (MCE) Benznidazole

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Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Benznidazole

Anti-Infection Compound Library

FDA-Approved Drug Library

Drug Repurposing Compound Library

Anti-COVID-19 Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Drug-Induced Liver Injury (DILI) Compound Library

Antiparasitic Compound library

Human Metabolite Library

Off-patent Drug Library

Mitochondrial Toxicity Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Publications Citing Use of MedChemExpress (MCE) Benznidazole

All Parasite Isoforms

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Benznidazole Related Classifications

HY-B1548 Related Classifications