Diethylamine NONOate diethylammonium salt
Based on 1 Customer Validation
Diethylamine NONOate (DEA NONOate, diethylammonium salt) is a nitric oxide donor. Diethylamine NONOate is a potent antimicrobial agent, which can inhibit Escherichia coli growth. Diethylamine NONOate also can enhance preservation of the donor rat heart.
For research use only. We do not sell to patients.
- CAS No.: 372965-00-9
- Formula: C8H22N4O2
- Molecular Weight:206.29
-
Storage:
-80°C, protect from light, stored under nitrogen
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-136278
DETA NONOate -
HY-18729A
L-NAME hydrochloride -
HY-101255
ODQ -
HY-K0301
Cell Counting Kit-8 -
HY-12318
IBMX -
HY-15025
Sildenafil -
HY-D0717
DAF-FM DA -
HY-121526
S-Nitroso-N-acetyl-DL-penicillamine -
HY-K0010
Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) -
HY-18731
1400W Dihydrochloride -
HY-101394
Spermine NONOate -
HY-W507393
Dipropylenetriamine NONOate
Biological Activity
Description
In Vitro
Diethylamine NONOate (8 and 65 mM; 0-20 h) diethylammonium salt shows the greatest inhibitory effect on E. coli growth at a dose of 65 mM for 20 h, resulting in a 99.9% reduction in E. coli[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 372965-00-9
-
Appearance Solid
-
Molecular Weight 206.29
-
Formula C8H22N4O2
-
Color White to light brown
-
SMILES
CCNCC.O=NN(O)N(CC)CC
-
Synonyms
DEA NONOate diethylamine
-
Shipping
Shipping with dry ice.
-
Storage
-80°C, protect from light, stored under nitrogen
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (484.75 mM)
DMSO : 50 mg/mL (242.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (6.06 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (6.06 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (555 KB)
- English - EN (555 KB)
- Français - FR (555 KB)
- Deutsch - DE (555 KB)
- Norwegian - NO (555 KB)
- Español - ES (555 KB)
- Swedish - SV (555 KB)
- Italian - IT (555 KB)
- Korean - KR (555 KB)
- Portuguese - PT (555 KB)
-
Handling Instructions (2659 KB)
References
[1]. Sysel AM, et al. Antimicrobial properties of diethylamine NONOate, a nitric oxide donor, against Escherichia coli: a pilot study. J Antibiot (Tokyo). 2021 Apr;74(4):260-265. [Content Brief]
[2]. Du ZY, et al. The nitric oxide donor, diethylamine NONOate, enhances preservation of the donor rat heart. J Heart Lung Transplant. 1998 Nov;17(11):1113-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.8475 mL | 24.2377 mL | 48.4754 mL | 121.1886 mL |
| 5 mM | 0.9695 mL | 4.8475 mL | 9.6951 mL | 24.2377 mL | |
| 10 mM | 0.4848 mL | 2.4238 mL | 4.8475 mL | 12.1189 mL | |
| 15 mM | 0.3232 mL | 1.6158 mL | 3.2317 mL | 8.0792 mL | |
| 20 mM | 0.2424 mL | 1.2119 mL | 2.4238 mL | 6.0594 mL | |
| 25 mM | 0.1939 mL | 0.9695 mL | 1.9390 mL | 4.8475 mL | |
| 30 mM | 0.1616 mL | 0.8079 mL | 1.6158 mL | 4.0396 mL | |
| 40 mM | 0.1212 mL | 0.6059 mL | 1.2119 mL | 3.0297 mL | |
| 50 mM | 0.0970 mL | 0.4848 mL | 0.9695 mL | 2.4238 mL | |
| 60 mM | 0.0808 mL | 0.4040 mL | 0.8079 mL | 2.0198 mL | |
| 80 mM | 0.0606 mL | 0.3030 mL | 0.6059 mL | 1.5149 mL | |
| 100 mM | 0.0485 mL | 0.2424 mL | 0.4848 mL | 1.2119 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Diethylamine NONOate diethylammonium salt Related Classifications
HY-131925 Related Classifications
Powered by Bioz