1. Others
  2. Fluorescent Dye
  3. DAF-FM DA

DAF-FM DA  (Synonyms: Diaminofluorescein-FM diacetate)

Cat. No.: HY-D0717 Purity: ≥98.0%
COA Handling Instructions

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).

For research use only. We do not sell to patients.

DAF-FM DA Chemical Structure

DAF-FM DA Chemical Structure

CAS No. : 254109-22-3

Size Price Stock Quantity
Solvent
100μg (5 mM * 40 μL in DMSO) USD 180 In-stock
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm)[1].

In Vitro

The BCa cells are pre-incubated with 5 μM DAF-FM DA for 30 min to load the probe. Subsequently, the cells were incubated with fresh Hank's buffer for 20 min to complete de-esterification. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

496.42

Formula

C25H18F2N2O7

CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

515

Excitation (Ex)

495

SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: ≥98.0%

Dyeing Example
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
DAF-FM DA
Cat. No.:
HY-D0717
Quantity:
MCE Japan Authorized Agent: