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  3. Fluorescent Dye
  4. DAF-FM DA

DAF-FM DA  (Synonyms: Diaminofluorescein-FM diacetate)

Cat. No.: HY-D0717 Purity: ≥98.0%
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DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).

For research use only. We do not sell to patients.

DAF-FM DA Chemical Structure

DAF-FM DA Chemical Structure

CAS No. : 254109-22-3

Size Price Stock Quantity
Solvent
100μg (5 mM * 40 μL in DMSO) USD 180 In-stock
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Based on 7 publication(s) in Google Scholar

DAF-FM DA Related Antibodies

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Description

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm)[1].

In Vitro

The BCa cells are pre-incubated with 5 μM DAF-FM DA for 30 min to load the probe. Subsequently, the cells were incubated with fresh Hank's buffer for 20 min to complete de-esterification. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DAF-FM DA Related Antibodies
In Vivo

Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

496.42

Formula

C25H18F2N2O7
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

515
Excitation (Ex)

495
SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: ≥98.0%

COA (256 KB)

Handling Instructions (2659 KB)
Dyeing Example
References
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DAF-FM DA Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAF-FM DA254109-22-3Diaminofluorescein-FM diacetateFluorescent DyeNOInhibitorinhibitorinhibit

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