DNA Gyrase-IN-18 

DNA Gyrase-IN-18 (Compound 6B) is a DNA Gyrase inhibitor and Antibacterial agent. DNA Gyrase-IN-18 significantly inhibits the supercoiling activity of DNA Gyrase. DNA Gyrase-IN-18 inhibits biofilm formation by *Staphylococcus aureus*. DNA Gyrase-IN-18 exhibits excellent antibacterial activity, with a MIC of 0.125 μg/mL against Staphylococcus aureus and a MIC of 0.5 μg/mL against Mycobacterium tuberculosis^[1].

DNA Gyrase-IN-18 (0.03-64 μg/mL; 18-24 h) potently inhibits growth of Staphylococcus aureus (ATCC 29213) with a MIC of 0.125 μg/mL after 18-24 h incubation at 37 °C^[1].
DNA Gyrase-IN-18 (0.03-64 μg/mL; 7 days) potently inhibits growth of Mycobacterium tuberculosis (MC 26230) with a MIC of 0.5 μg/mL after 7 days incubation at 37 °C^[1].
DNA Gyrase-IN-18 (0.19-200 μg/mL; 24 h) shows low cytotoxicity against HepG2 cells with a CC₅₀ of 103.6 μg/mL, yielding a high selectivity index of 828.8 for Staphylococcus aureus (ATCC 29213)^[1].
DNA Gyrase-IN-18 (0.25-128 μg/mL; 48 h) inhibits biofilm formation by Staphylococcus aureus (ATCC 29213) with an MBIC₅₀ of 0.309 μg/mL after 48 h static incubation at 37 °C^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

422.28

C₂₀H₁₆BrN₅O

O=C(N/N=C\C1=CNC2=C1C=CC=C2)CN3C=CC(C4=CC=C(C=C4)Br)=N3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bellapukonda SM, et al. Exploration of 1,3-di substituted pyrazole conjugates as DNA gyrase inhibitors to combat Staphylococcus aureus and Mycobacterium tuberculosis. Bioorg Chem. Published online March 4, 2026.