Ca2+ Indicator Probes

INDO 1 pentasodium is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a K_D·β value of 0.44 μM. INDO 1 pentasodium binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca²⁺, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca²⁺ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).

INDO 1 is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a K_D·β value of 0.44 μM. INDO 1 binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca²⁺, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca²⁺ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).

Rhod-FF AM is a fluorescent calcium indicator. Rhod-FF AM is cell permeable and is hydrolyzed by intracellular esterases to generate Rhod-FF.

5,5'-Dibromo BAPTA tetrapotassium is a calcium chelator, can be formulated into a buffer with a defined calcium ion concentration. 5,5'-Dibromo BAPTA tetrapotassium can control the concentration of calcium ions inside and outside the cell and can be used in studies of calcium physiological activity.

5,5'-Dibromo-BAPTA is a biochemical assay reagent.

Fluo-2 AM (Fluo-2 acetoxymethyl ester) is a fluorescent dye, that can be used for detection of intracellular calcium ion concentration.

Rhod-2 potassium is a cell impermeant, red fluorescent calcium indicator. Rhod-2 potassium exhibits a significant shift in fluorescence intensity upon calcium binding (ex max=549 nm; calcium-free v. ex/em max=552/581 nm; calcium-bound). Unlike the UV-excitable indicators Fura-2 and Indo-1 (HY-D0121), there is no accompanying spectral shift.

Fluo-3 pentaammonium is a cell-impermeable calcium ion (Ca²⁺) indicator. Fluo-3 pentaammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).

Fluo-3FF pentapotassium is a cell-impermeant fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds=42 and 0.4 μM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.

5,5'-Difluoro BAPTA tetrapotassium is a difluoro-derivative of BAPTA (HY-100168). 5,5'-Difluoro BAPTA tetrapotassium shows large ¹⁹F NMR chemical shifts upon chelating divalent cations. 5,5'-Difluoro BAPTA tetrapotassium has high selectivity for Ca²⁺. 5,5'-Difluoro BAPTA tetrapotassium can inhibit the growth of pollen tube.

5,5'-Dimethyl BAPTA tetrapotassium is a water-soluble, extracellular membrane metal chelator with relative selectivity for calcium ions.

5-Nitro BAPTA tetramethyl ester is a calcium chelator. 5-Nitro BAPTA tetramethyl ester involves in the two-photon probe synthesis, and is used for real-time imaging of intracellular calcium ions, calcium waves monitoring at a depth of 100-300 μm in liver tissues for 1100-4000 s. 5-Nitro BAPTA tetramethyl ester, together with fluorescent compound 2-Me-substituted TM, can be used to form a red fluorescent probe (CaTM-2 AM).

5-Nitro BAPTA is a calcium chelator, combinded with 2-Me-substituted TM ( as a fluorescent moiety), can be used to form a red fluorescent probe (CaTM-2 AM), for imaging of cytoplasmic Ca²⁺ in cultured living cells. 5-Nitro BAPTA is a building block used in the synthesis of Ca²⁺ specific chelators, Ca²⁺ buffers, and fluorescent Ca²⁺ indicators.

Calcium Green BAPTA-2 AM is a fluorescent calcium indicator.

5-Bromo-5'-methyl BAPTA tetramethyl ester is a derivative of BAPTA, which is a calcium indicator suitable for measurement of relatively high level of calcium.

Fura PE-3 potassium is a Ca²⁺sensitive fluorophore. Fura PE-3 potassium loads in IPA (intrapulmonary arteries) and MA (mesenteric resistance arteries) for the research of vasoconstriction.

FURA-PE3/AM is a leak-resistant fluorescent calcium indicator dye.

Coelenterazine hcp is a Coelenterazine analogue. Coelenterazine hcp shows high sensitivity to Ca²⁺. Coelenterazine hcp is suited for monitoring intracellular Ca²⁺.

BTC-AM is a low affinity calcium indicator. BTC-AM has substantial calcium-independent fluorescence at all excitation wavelengths. BTC-AM is readily loaded into neurons and is rapidly hydrolysed.

BTC tetrapotassium is a cell-impermeant calcium indicator (K_d approximately 7-26 µM) featuring many desirable properties for cellular calcium imaging, including long excitation wavelengths (400/485 nm), low sensitivity to Mg²⁺, and accuracy of ratiometric measurement.