Firefly luciferase-IN-1
Based on 1 Customer Validation
Firefly luciferase-IN-1 is a cell-permeable firefly luciferase inhibitor with an IC50 of 0.25 nM and a Ki of 1.72 nM. Firefly luciferase-IN-1 reversibly binds to the D-luciferin-occupied pocket of Fluc, competing with D-luciferin. Firefly luciferase-IN-1 effectively blocks Fluc/D-Luciferin (HY-12591A)-induced bioluminescence.
For research use only. We do not sell to patients.
- Purity : 99.57%
- CAS No.: 2765796-41-4
- Formula: C19H16O3
- Molecular Weight:292.33
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
firefly luciferase 0.25 nM (IC50) |
firefly luciferase 1.72 nM (Ki) |
In Vitro
Firefly luciferase-IN-1 (compound 48) is a highly potent inhibitor of purified Firefly luciferase, with an IC50 of 0.25 nM[1].
Firefly luciferase-IN-1 (20 μM) selectively inhibits Fluc without affecting Rluc activity, and its inhibitory effect is not due to absorption of luminescence[1].
Firefly luciferase-IN-1 (1.0 μM) reversibly inhibits Firefly luciferase by competitively binding to the D-luciferin pocket, with a KI of 1.72 nM[1].
Firefly luciferase-IN-1 inhibits Fluc in transiently transfected 293T cells with an IC50 of 2.6 nM and an IC50 of 15 nM in cell lysates[1].
Firefly luciferase-IN-1 (compound 48) potently inhibits intracellular Fluc in PC9-Fluc, A549-Fluc, H1299-Fluc, Hepa1-6-Fluc, and BEAS2B-Fluc cell lines, with IC50 values ranging from 2.1 to 32 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c-nu (female)[1]
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Dosage:0.5 nmol
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Administration:intratumoral; single administration
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Result:Reduced residual bioluminescence to 2.8%.
Chemical Information
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CAS No. 2765796-41-4
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Appearance Solid
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Molecular Weight 292.33
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Formula C19H16O3
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Color Light yellow to yellow
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SMILES
O=C1C2=CC=C(C(O)=O)C=C2CC/C1=C\C3=CC=C(C=C3)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (342.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.4208 mL | 17.1040 mL | 34.2079 mL | 85.5198 mL |
| 5 mM | 0.6842 mL | 3.4208 mL | 6.8416 mL | 17.1040 mL | |
| 10 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL | 8.5520 mL | |
| 15 mM | 0.2281 mL | 1.1403 mL | 2.2805 mL | 5.7013 mL | |
| 20 mM | 0.1710 mL | 0.8552 mL | 1.7104 mL | 4.2760 mL | |
| 25 mM | 0.1368 mL | 0.6842 mL | 1.3683 mL | 3.4208 mL | |
| 30 mM | 0.1140 mL | 0.5701 mL | 1.1403 mL | 2.8507 mL | |
| 40 mM | 0.0855 mL | 0.4276 mL | 0.8552 mL | 2.1380 mL | |
| 50 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7104 mL | |
| 60 mM | 0.0570 mL | 0.2851 mL | 0.5701 mL | 1.4253 mL | |
| 80 mM | 0.0428 mL | 0.2138 mL | 0.4276 mL | 1.0690 mL | |
| 100 mM | 0.0342 mL | 0.1710 mL | 0.3421 mL | 0.8552 mL |
Firefly luciferase-IN-1 Related Classifications
HY-155945 Related Classifications
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