AUTEN-67
Based on 1 Customer Validation
AUTEN-67 (Autophagy enhancer-67) is an orally active autophagy enhancer and MTMR14 inhibitor. AUTEN-67 has anti-aging and neuroprotective effects. AUTEN-67 protects neurons from stress-induced cell death. AUTEN-67 also restores nesting behavior in a mice model of Alzheimer disease.
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- Pureza: 97.14%
- No. CAS: 1783800-77-0
- Fòrmula: C23H14N4O6S
- Peso molecular:474.45
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Autophagy Compound Library
HY-L029
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Anti-Aging Compound Library
HY-L034
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Oxygen Sensing Compound Library
HY-L045
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Anti-Alzheimer's Disease Compound Library
HY-L069
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Neuroprotective Compound Library
HY-L070
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Neurodegenerative Disease-related Compound Library
HY-L086
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Actividad biológica
AUTEN-67 (2-100 µM, 3 h) inhibits MTMR14 by nearly 3%-70%, induces autophagic flux, and promotes the survival in HeLa cells[1].
AUTEN-67 (10-100 µM, 2 h) induces autophagy in Drosophila via inhibiting EDTP[1].
AUTEN-67 (1-50 µM) decreases levels of LC3B-II, and protects neurons from oxidative stress (increases cell viability) in murine primary neurons[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
AUTEN-67 (19 mg/kg, p.o., 3 times a week, for 3 month) restores nesting behavior and decreases APP level in the Alzheimer disease mice model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Alzheimer disease model (Mice expressing the human APP)[1]
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Dosage:19 mg/kg, 3 times a week for 3 month
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Administration:Oral administration
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Result:Restored nesting behavior by around 30%.
Decreased Amyloid β levels in the hemibrain of mice.
Chemical Information
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No. CAS 1783800-77-0
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Appearance Solid
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Peso molecular 474.45
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Fòrmula C23H14N4O6S
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Color Orange to red
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SMILES
O=S(C1=CC=C([N+]([O-])=O)C=C1)(NC(C2=O)=C(N3C4=CC=CC=C4N=C3)C(C5=C2C=CC=C5)=O)=O
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Synonyms
Autophagy enhancer-67
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
DMSO : 11.9 mg/mL (25.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.63 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1077 mL | 10.5385 mL | 21.0770 mL | 52.6926 mL |
| 5 mM | 0.4215 mL | 2.1077 mL | 4.2154 mL | 10.5385 mL | |
| 10 mM | 0.2108 mL | 1.0539 mL | 2.1077 mL | 5.2693 mL | |
| 15 mM | 0.1405 mL | 0.7026 mL | 1.4051 mL | 3.5128 mL | |
| 20 mM | 0.1054 mL | 0.5269 mL | 1.0539 mL | 2.6346 mL | |
| 25 mM | 0.0843 mL | 0.4215 mL | 0.8431 mL | 2.1077 mL |
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