Deoxymikanolide
Deoxymikanolide is an orally active antibacterial agent that inhibits catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activities. Deoxymikanolide increases glycan metabolism, phosphorus metabolism, electric conductivity, intrabacterial reactive oxygen species (ROS) levels, and malondialdehyde (MDA) levels, causes cell shrinkage, cytoplasmic damage, and cell disruption in Ralstonia solanacearum. Deoxymikanolide inhibits Acetic acid-induced writhing in mice. Deoxymikanolide can be used for the research of bacterial wilt.
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- No. CAS: 23753-57-3
- Fòrmula: C15H16O5
- Peso molecular:276.28
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
In Vitro
Deoxymikanolide (15.625-125 mg/L; measured at 2-hour intervals) inhibits the growth of Ralstonia solanacearum race #4 in a concentration-dependent manner, with complete suppression at higher concentrations[1].
Deoxymikanolide (125 mg/L; 2-24 h) induces time-dependent ultrastructural damage to Ralstonia solanacearum race #4, including cell shrinkage, cytoplasmic leakage, and abnormal cell shapes, with complete cytoderm thickening at 24 h[1].
Deoxymikanolide (62.5-125 mg/L; measured every 2 h for 24 h) significantly increases the phosphorus utilization ratio of Ralstonia solanacearum race #4[1].
Deoxymikanolide (62.5-125 mg/L; measured at 5-hour intervals) inhibits the activities of CAT, POD, and SOD in Ralstonia solanacearum race #4, with SOD inhibition rates of 73.50% (62.5 mg/l) and 86.41% (125 mg/L) at 10 min[1].
Deoxymikanolide (62.5-125 mg/L; 24 h) induces significant intracellular ROS accumulation in Ralstonia solanacearum race #4, exceeding levels induced by 340 mg/l H2O2[1].
Deoxymikanolide (62.5-125 mg/L; 24 h post-treatment) increases MDA levels in Ralstonia solanacearum race #4 at 24 h, indicating lipid peroxidation from excessive ROS[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Swiss mice (male, 20-25 g)[2]
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Dosage:10 mg/kg
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Administration:p.o.
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Result:Reduced acetic acid-induced writhing to 8.5 and produced a 67.3% inhibition of writhing compared to the control group (26.0 writhings).
Chemical Information
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No. CAS 23753-57-3
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Peso molecular 276.28
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Fòrmula C15H16O5
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SMILES
C=C1[C@]2([H])[C@](OC1=O)([H])C[C@]3([C@@](CCC4=C[C@@]2([H])OC4=O)([H])O3)C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Deoxymikanolide Related Classifications
HY-N13756 Related Classifications
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Keywords
- Deoxymikanolide
- 23753-57-3
- Bacterial
- Reactive Oxygen Species (ROS)
- SOD
- ROS
- mice
- electric conductivity
- MDA
- peroxidase
- CAT
- superoxide dismutase
- phosphorus metabolism
- malondialdehyde
- bacterial wilt
- Mikania micrantha Bunge
- male Swiss mice
- glycan metabolism
- Ralstonia solanacearum race #4
- catalase
- Ralstonia solanacearum
- POD
- intrabacterial reactive oxygen species
- Inhibitor
- inhibitor
- inhibit